Effect of Glucose at High Concentrations on the Apoptosis of the Cultured Periodontal Ligament Cells and Osteoblasts

고농도 포도당이 뼈모세포와 치주인대세포의 세포자멸사에 미치는 영향에 관한 연구

  • Park, Sung-Ho (Department of Oral Anatomy, School of Dentistry, Kyung Hee University) ;
  • Jue, Seong-Suk (Department of Oral Anatomy, School of Dentistry, Kyung Hee University) ;
  • Hong, Jung-Pyo (Department of Oral Medicine, School of Dentistry, Kyung Hee University) ;
  • Shin, Je-Won (Department of Oral Anatomy, School of Dentistry, Kyung Hee University)
  • 박성호 (경희대학교 치의학전문대학원 구강해부학교실) ;
  • 주성숙 (경희대학교 치의학전문대학원 구강해부학교실) ;
  • 홍정표 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 신제원 (경희대학교 치의학전문대학원 구강해부학교실)
  • Published : 2007.12.30

Abstract

This experiment was designed to clarify the effect of extracellular glucose on the osteoblasts and periodontal ligament cells. The cells were incubated for 24 and 48 hours with ${\alpha}$-MEM including 1,000 mg/L (control group) and 4,500 mg/L (experimental group) of glucose. Then, the expressions of caspase-3, p38 MAPK, JNK-1, and ERK-1 were examined using Elisa assay and Western blot. The results were as follows: 1. The expression of caspase-3 and p38 MAPK was increased by the high extracellular glucose in both cells. 2. The expression of caspase-3 and p38 MAPK was increased greatly in the periodontal ligament cells than the E1 cells by the high extracellular glucose. 3. The expression of JNK-1 was increased by the high extracellular glucose in both cells. 4. The expression of ERK-1 was not changed by the high extracellular glucose in both cells. These results suggest that extracellular glucose at high concentrations may inhibit the periodontal regeneration process increasing cellular apoptosis. And p38 MAPK and JNK-1 pathway may be the most responsible intracellular pathway rather than ERK-1.

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