The Effect of Herbal Formulation (OPB), Rehmannia Glutinosa Libosch and Eleutherococcus Senticosus Max Extracts on Bone Density and Bone Biochemical Marker in Ovariectomized Rats

숙지황과 가시오가피 복합추출물(OPB)이 난소절제 흰쥐의 골밀도 및 골대사에 미치는 영향

  • Kim, Jung-Keun (Dept. of Pharmacology analysis, Research Institute, Oscotec Inc.) ;
  • Kim, Se-Won (Dept. of Pharmacology analysis, Research Institute, Oscotec Inc.) ;
  • Lee, Byung-Eui (Dept. of Herbal medicine, Research Institute, Oscotec Inc.) ;
  • Hwang, Hyeon-Hwan (Dept. of Safety and Toxicology, Research Institute, Oscotec. Inc.) ;
  • Kwon, Jong-Seok (Dept. of Pharmacology analysis, Research Institute, Oscotec Inc.) ;
  • Ko, Seon-Yle (Dept. of Pharmacology analysis, Research Institute, Oscotec Inc.)
  • 김정근 ((주)오스코텍 약효검색팀) ;
  • 김세원 ((주)오스코텍 약효검색팀) ;
  • 이병의 ((주)오스코텍 천연물의약팀) ;
  • 황현환 ((주)오스코텍 약동학팀) ;
  • 권종석 ((주)오스코텍 약효검색팀) ;
  • 고선일 ((주)오스코텍 약효검색팀)
  • Published : 2007.03.30

Abstract

This study was conducted to investigate the preventing effects of OPB (water extracts of Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max) on bone loss in ovariectomized rats. Twenty Sprague Dawley rats of 13 week-old were divided into two groups: control group (ovariectomized, OVX)) and experimental group (OVX + OPB). The preventing effects of OPB on bone loss, OPB were fed with 100 mg OPB/kg body weight from 3 days after ovariectomization. The duration of the treatment period was 8 weeks. All bone mineral density, bone mineral content indices and bone strength indices measured by peripheral quantitative computerized tomography (pQCT) and serum bone marker assessment were carried out at end of experiment. pQCT scanning showed that OVX induced a significant decrease in cancellous bone mineral density in the proximal tibia ($-29.8{\pm}3.0%$). These decreases were significantly prevented by the administration of OPB 100 mg/kg ($-21.4{\pm}2.3%$. p<0.05). Bone strength indices showed significant difference between OVX and OPB treated rats (anti-fracture, anti-twisting, p<0.05). These data suggested that administration of OPB inhibited the loss of bone in OVX rats. CTx level were lower than in the OPB-treated animals compared with OVX. However there was no significant difference between OVX and OPB treated OVX rat. Our results suggest that OPB is effective in preventing the development of bone loss induced by ovariectomy in rats.

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