Analysis of the ADSF/resistin Gene Polymorphism Associated with Carcass Traits in Hanwoo

한우 ADSF/resistin 유전자의 단일 염기 다형과 육질관련형질 상관 분석

  • Park, J.A. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Kang, H.K. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Chae, E.J. (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Seo, K.S. (National Institute of Animal Science, RDA) ;
  • Kim, S.H. (Department of Biology, Kyung Hee University) ;
  • Yun, C.H. (School of Agricultural Biotechnology, Seoul National University) ;
  • Moon, Y.S. (Department of Animal Science and Biotechnology, Jinju National University)
  • 박지애 (진주산업대학교 동물생명과학과) ;
  • 강혜경 (진주산업대학교 동물생명과학과) ;
  • 채은진 (진주산업대학교 동물생명과학과) ;
  • 서강석 (축산과학원) ;
  • 김상훈 (경희대학교 생물학과) ;
  • 윤철희 (서울대학교 식품동물생명공학부) ;
  • 문양수 (진주산업대학교 동물생명과학과)
  • Published : 2007.10.31


Adipocyte-specific secretory factor(ADSF)/resistin, an hormone, is a small cysteine-rich protein secreted from adipose tissue and ADSF/resistin has been implicated in modulating adipogenesis in human and rodents. Although the exact role of ADSF/resistin in bovine has not been identified, it may have directly or indirectly involved in adipocyte differentiation. The objective of this study was to investigate its DNA polymorphism associated with carcass traits in Korean Native Cattle(Hanwoo). To investigate DNA polymorphism in Hanwoo ADSF/resistin gene, blood samples were taken from 295 Hanwoo steers belonging to progeny testing at Hanwoo Improvement Center in Korea. Seven single nucleotide polymorphisms(SNPs) were found in intron regions but not in any other regions including promoter (1.7kb) and 4 exons. The highest frequency among SNPs was C186A(0.16/0.84) following G964A (0.156/0.884). The significant correlation(P<0.05) between the SNPs and economic traits was found on 764Ains associated with marbling but not from any other SNPs determined.  A computer simulation was also conducted to assess the efficiency of marker assisted selection(MAS) versus the conventional breeding scheme.  Results revealed that MAS was more efficient as a breeding tool compared to the conventional. In conclusion, ADSF/Resistin gene is one of candidate genes to evaluate the quality, especially marbling score, in Hanwoo.


  1. Ahima, R. S. and Flier, J. S. 2000. Adipose Tissue as an Endorcine Organ. Trends Endocrinol Metab, 11(8):327-332
  2. Degawa-Yamauchi M., Bovenkerk, J. E., Juliar, B. E., Watson, W., Kerr, K., Jones, R., Zhu, Q. and Considine, R. V. 2003. serum resistin (FIZZ3) protein is increased in obese human. J Clin Endocrinol Metab. 88(11):5452-5455
  3. Duman, BS, Cagatay, P, Haterni, H. and Ozturk, M. 2007. Association of Resistin Gene 3'-Untranslated Region EX4-44G-->A Polymorphism with Obesity and Insulin-Related Phenotypes in Turkish Type 2 Diabetes Patients. Rev Diabet Stud. 4(1):49-55
  4. Engert, JC., Vohl, MC., Williams, SM., Lepage, P., Loredo-Osti JC., Faith, J., Dore, C., Renaud, Y., Burtt, NP., Villeneuve, A., Hirschhorn, JN., Altshuler, D., Groop, LC., Despres, JP., Gaudet, D. and Hudson, TJ. 2002. 5' flanking variants of resistin are associated with obesity. Diabetes. 51(5):1629-1634
  5. Gregoire, F. M., Smas, C. M. and Sul, H. S. 1998. Understanding adipocyte differentiation. Physiol. Rev. 78(3):783-809
  6. Kang, H. K., Park, J. A., Seo, K. S., Kim, S. H., Choi, Y. J. and Moon, Y. S. 2006. Characteristics of structure and expression pattern of ADSF/resistin gene in Korean native cattle. Asian-Aust. J. Anim. Sci. Vol 19, No. 3:329-334
  7. Kawada, Y., Lee, S. E., Chen, C., Limson, B. M., Chan, P., Kongkanand, A., Wudhikam, S., Tung, K. H., Rahardjo, P. and Saito, I. 1996. Evaluation of clinical efficacy of antimicrobials in complicated urinary tract infection. Comparison of Japanese criteria with IDSA guidelines. Infectious Diseases Society of America. Drugs. 49 (Suppl 2):362-364
  8. Kim, K. H., Lee, K., Moon, Y. S. and Sul, H. S. 2001. A Cysteinerich Adipose Tissue-specific Secretory Factor Inhibits Adipocyte Differentiation. Journal of Biological Chemistry. 276(14):11252-11256
  9. Kim, K. H., Zhao, L., Moon, Y., Kang, C. and Sul, H. S. 2003. Dominant inhibitory adipocyte-specific secretory factor(ADSF)/resistin enhances adipogenesis and improves insulin sensitivity. PNAS. 101(17):6780-6785
  10. Kunnari, A., Ukkola, O. and Kesaniemi, YA. 2005. Resistin polymorphisms are associated with cerebrovascular disease in Finnish Type 2 diabetic patients. Diabet Med. 22(5):583-589
  11. Norata, GD., Ongari, M., Garlaschelli, K., Tibolla, G., Grigore, L., Raselli, S., Vettoretti, S., Baragetti, I., Noto, D., Cefalu, AB., Buccianti, G., Averna, M. and Catapano, AL. 2007. Effect of the -420C/G variant of the resistin gene promoter on metabolic syndrome, obesity, myocardial infarction and kidney dysfunction. J Intern Med. 262(1):104-112
  12. Oehi, M., Osawa, H., Hirota, Y., Hara, K., Tabara, Y., Tokuyama, Y., Shimizu, I., Kanatsuka, A., Fujii, Y., Ohashi, J., Miki, T., Nakamura, N., Kadowaki, T., Itakura, M., Kasuga, M. and Makino, H. 2007. The frequency of the G/G genotype of resistin single nucleotide polymorphism at -420 appears to be increased in younger onset type 2 diabetes Diabetes. Aug 13; [Epub ahead of print]
  13. Osawa, H., Yamada, K., Onuma, H., Murakami, A., Ochi, M., Kawata, H., Nishimiya, T., Niiya, T., Shimizu, I., Nishida, W., Hashiramoto, M., Kanatsuka, A., Fujii, Y., Ohashi, J. and Makino, H. 2004. The G/G genotype of a resistin single-nucleotide polymorphism at -420 increases type 2 diabetes mellitus susceptibility by inducing promoter activity through specific binding of Sp1/3. Am J Hum Genet. 75(4):678-686
  14. Osawa, H., Onuma, H., Ochi, M., Murakami, A., Yamauchi, J., Takasuka, T., Tanabe, F., Shimizu, I., Kato, K., Nishida, W., Yamada, K., Tabara, Y., Yasukawa, M., Fujii, Y., Ohashi, J., Miki, T. and Makino, H. 2005. Resistin SNP-420 determines its monocyte mRNA and serum levels inducing type 2 diabetes. Biochem Biophys Res Commun. 335(2):596-602
  15. Pizzuti, A., Argiolas, A., Di Paola R., Baratta, R., Rauseo, A., Bozzali, M., Vigneri, R., Dallapiccola, B., Trischitta, V. and Frittitta, L. 2002. An ATG repeat in the 3'-untranslated region of the human resistin gene is associated with a decreased risk of insulin resistance. J Clin Endocrinol Metab. 87(9):4403-4406
  16. SAS. institute, 2003. SAS/STAT user's guide: Statistics, version 9.1. SAS Instatute Inc. Cary, NC
  17. Sentinelli, F., Romeo, S., Arca, M., Filippi, E., Leonetti, F., Banchieri, M., Di Mario U. and Baroni, MG. 2002. Human resistin gene, obesity, and type 2 diabetes: mutation analysis and population study. Diabetes. 51(3):860-862
  18. Steppan, C. M., Bailey, S. T., Bhat, S., Brown, E. J., Banerjee, R. R., Wright, C. M., Patel, H. R., Ahima, R. S. and Lazar, M. A. 2001. The hormone resistin links obesity to diabetes. Nature. 409(6818):307-312
  19. Steppan, C. M. and Lazar, M. A. 2002. Resistin and obesity-associated insulin resistance. TRENDS in Endocrinology & Metabolism. 13(1):18-23
  20. Wang, H., Chu, WS., Hemphill, C. and Elbein, SC. 2002. Human resistin gene: molecular scanning and evaluation of association with insulin sensitivity and type 2 diabetes in Caucasians. J. Clin Endocrinol Metab. 87(6):2520-2524
  21. Zhang, Y., Proenca, R., Maffel, M., Barone, M., Leopold, L. and Friedman, J. M. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372:425-432