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Isolation and Characterization of vasa Gene of Triploid and Diploid Human Lung Flukes (Paragonimus westermani)

폐흡충의 이배체와 삼배체 vasa 유전자 분석 및 특징

  • Lee, Keun-Hee (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Yu, Hak-Sun (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Hur, Jae-Won (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Yu, Sung-Suk (Department of Life Science Gwangju Institute of Science and Technology) ;
  • Choi, Sun-Hee (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Park, Sang-Kyun (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Lee, Sun-Joo (Department of Parasitology, School of Medicine, Pusan National University) ;
  • Chung, Dong-Il (Department of Parasitology, School of Medicine, Kyungpook National University) ;
  • Kong, Hyun-Hee (Department of Parasitology, School of Medicine, Kyungpook National University) ;
  • Ock, Mee-Sun (Department of Parasitology, Kosin University College of Medicine) ;
  • Jeong, Hae-Jin (Department of Parasitology, School of Medicine, Pusan National University)
  • 이근희 (부산대학교 의과대학 기생충학 교실) ;
  • 유학선 (부산대학교 의과대학 기생충학 교실) ;
  • 허재원 (부산대학교 의과대학 기생충학 교실) ;
  • 유성숙 (광주과학기술연구소) ;
  • 최선희 (부산대학교 의과대학 기생충학 교실) ;
  • 박상균 (부산대학교 의과대학 기생충학 교실) ;
  • 이선주 (부산대학교 의과대학 기생충학 교실) ;
  • 정동일 (경북대학교 의과대학 기생충학 교실) ;
  • 공현희 (경북대학교 의과대학 기생충학 교실) ;
  • 옥미선 (고신대학교 의과대학 기생충학 교실) ;
  • 정해진 (부산대학교 의과대학 기생충학 교실)
  • Published : 2007.04.25

Abstract

In this study, we isolated, characterized, and compared the vasa homologous genes of diploid and triploid Paragonimus westermani and localized VASA homologous proteins in both lung fluke types. Open reading frames of Pw-vasa-2n and Pw-vasa-3n were of 1812 bp, and encoded deduced proteins of 622 amino acids with calculated molecular weights of 69.0 kDa and 68.9 kDa and pI's of 9.11 and 9.03, respectively. A comparison of these two VASA deduced protein sequences showed that only 6 of the 622 amino acids differed. The deduced sequences of Pw-VASA-2n and Pw-VASA-3n contained eight consensus sequences characteristic of the DEAD-box protein family and their N-terminal regions contained four arginine-glycine-glycine (RGG) motifs. These two lung fluke VASA-like proteins were more similar to those of other VASA proteins than to those of other DEAD-family proteins isolated from several organisms (planarian, zebra fish, mouse, and human). vasa homologous gene transcription and VASA protein expressions in triploid type lung flukes was slightly stronger than in the diploid type. Immunostaining showed that testes and a portion of the ovaries of both diploid and triploid lung flukes reacted strongly to anti-Pw-VASA antibody.

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