Oh, Sae-Eun

  • Published : 2007.02.28


Two phase UASB reactors for treating wastewater with sulfate were operated to assess the performance and competition of organics between sulfate reducing bacteria(SRB) and methane producing bacteria(MPB), and the change of characteristics of microorganisms. The reactors were fed in parallel with a synthetic wastewater of 4,000-5,000 mgCOD/L and sulfate concentration of $800-1,000\;mgSO_4/L$. In the MPR(methane producing reactor) and CR(control reactor), COD removal efficiencies were 90% and 60%, respectively, at the OLR(organic loading rate) of 6 gCOD/L, while the amount of biogas and methane content were 6.5 L/day and 80%, and 3 L/day and 50%, respectively. However, the portion of electron flow used by SRB at the OLR of 6 gCOD/L day in MPR and CR was 3% and 26%, respectively. This indicated that the increase of OLR of wastewater containing high sulfate like CR resulted in activity decrease and cell decay of MPB, while SRB was adapted immediately to new environment. The MPB activities in MPR and CR were 2 and $0.38\;kgCH_4-COD$/gVSS day at the OLR of 6 gCOD/L. This indicated hat SRB dominated gradually over MPB during long-term operation with wastewater containing sulfate as a consequence of outcompeting of SRB over MPB. In addition, the solution within AFR was maintained around pH 5.0, the MPB such as Methanothrix spp. which was very important to formation of granules was detached from the surface of granules due to the decrease of activity by limitation of substrate transportation into MPB. Therefore, a significant amount of sludge was washed out from the reactor.


Anaerobic treatment;UASB;Sulfate reducing bacteria;Methanogens;Sulfate


  1. Anderson, G. K., Donnelly, T., and McKeown, K. J., 'Identification and control of inhibition in anaerobic treatment of industrial wastewater,' Proc. Biochem., 17, 28-32 (1982)
  2. Visser, A., Gao, A., and Lettinga, G., 'Effects of short-term temperature increases on the mesophilic anaerobic break down of sulfate containing synthetic wastewater,' Water Res., 27(4), 541-550 (1993)
  3. Rinzema, A. and Lettinga, G., 'The effect of sulfide on the anaerobic degradation of propionate,' Environ. Technol. Lett., 9, 83 (1988)
  4. Koster, I. W., Rinzema, A., DeVegt, A. L., and Lettinga, G., 'Sulfide Inhibition of the methanogenic activity of granular sludge at various pH levels,' Water Res., 20, 1561-1567 (1986)
  5. Parkin, G. F., Lynch, N. L., Kuo, W. C., Van Kueren, E. L., and Bhattacharacy, S. K., 'Interaction between sulfate reducers and methanogens fed acetate and propionate,' J. WPCF, 62, 780 (1990)
  6. Anderson, G. K., Kasapgil, B., and Ince, O., 'Microbiological study of two-stage anaerobic digestion during start-up,' Water res., 28(11), 2383-2392 (1994)
  7. Shin, H. S., and Oh, S. E., 'Effect of sulfate and sulfide on anaerobic microorganism in anaerobic digester,' J. KSWQ, 12(2), 191-196 ( 1997)
  8. Shin, H. S., Oh, S. E., and Kwon, J. C., 'Activity of SRB and MPB at thermophilic condition in anaerobic digester treating wastewater with high sulfate concentration,' Kor. Soc. Environ. Eng., 19(3), 305-313 (1997)
  9. Tramper, J., Van 'Groenestijn, J. W., Luyben K. A. M., and Hulshoff Pol, L. W., 'Some physical and kinetic properties of granular anaerobic sludge,' In Innovations in Biotechnology, 145-155 (1984)
  10. APHA, 'Standard methods for the examination of water and wastewater,' 16th edn. American Public Health Association, Washington, DC., (1987)
  11. Visser, A., Alphenaar, P., A., Gao, Y., van Rossum, G., and Lettinga, G., 'Granulation and immobilisation of methanogenic and sulfate-reducing bacteria in high-rate anaerobic reactor,' Appl., Microbiol. Biotechnol., 40, 575-581 (1993)
  12. Valcke, D., and Verstrate, W., 'A practical method to estimate the acetoclastic methanogenic biomass in anaerobic sludge,' J. WPCF, 55, 1191-1195 (1993)
  13. Isa, Z., Grusenmeyer, S., and Verstraete, W., 'Sulfate reduction relative to methane production in high-rate anaerobic digestion: microbiological aspects,' Appl. Environ. Microbiol., 3, 580-587(1986b)
  14. Yoda, M., Kitagawa, M., and MiyaJi. Y., 'Granular sludge formation in the anaerobic expanded microcarrier process,' Water Sci. Tech., 21, 109-122(1989)
  15. Shin, H. S., Oh, S. E., and Lee, C. Y., 'Effect of temperature on activation of SRB and MPB in the anaerobic treatment of tannery wastewater containing high sulfate,' Kor. Soc. Environ. Eng., 18(1), 33-42 (1996)