Antimutagenic and Anticancer Effects of Leaf Mustard and Leaf Mustard Kimchi

  • Kim, Yong-Taek (R&D Center, Daesun Distiling Co. Ltd.) ;
  • Kim, Boh-Kyung (Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University) ;
  • Park, Kun-Young (Department of Food Science and Nutrition, and Kimchi Research Institute, Pusan National University)
  • Published : 2007.06.30


In this study, we investigated antimutagenic and anticancer activities of leaf mustard (LM, Brassica juncea) and leaf mustard kimchi (LMK) during their fermentation period. Methanol extracts were prepared from raw mustard, brined leaf mustard in 10% Gueun salt solution for 2 hrs, leaf mustard fermented at 15$^{\circ}C$ for 5 days after brined in 10% Guenun salt solution for 2 hrs (Fr-LM), fresh leaf mustard kimchi (Fresh-LMK) and optimally ripened leaf mustard kimchi fermented at 5$^{\circ}C$ for 30 days (OR-LMK). OR-LMK showed the strongest inhibitory activities against the mutagenicities induced by aflatoxin B1 in Salmonella Typhimurium TA100. LMs and LMKs inhibited the survival or growth of AGS human gastric adenocarcinoma cells and HT-29 human colon carcinoma cells in MTT assay and growth inhibition test. Among the extracts, OR-LMK and FR-LM exhibited strong antiproliferative effect against cancer cells, especially HT-29 cells. DAPI staining assay showed that OR-LMK induced apoptosis cell death of HT-29 cells in a dose-dependent manner. These results suggest that leaf mustards and leaf mustard kimchi have chemopreventive activities.


  1. Park KY, Lee KI, Rhee SH. 1992. Inhibitory effects of green-yellow vegetables on mutagenecity in Samonella assay system and on the growth of AZ-521 human gastric cancer cells. J Korean Soc Food Nutr 21: 149-153
  2. Park KY, Yang HS, Rhee SH, Rhew TH. 1993. Isolation, identification and biochemical mode of the antimutagenic and antitumor compounds from yellow-green vegetables and traditional anticancer medical plants. KOSEF report 90-0500-03
  3. Lee KI, Park KY, Rhee SH. 1992. Antimutagenic effect of green-yellow vegetables toward aflatoxin B1 and 4-butriqyubikube-1-oxide. J Korean Soc Food Nutr 21: 143- 148
  4. Lee KI. 1992. Inhibitory effects of green-yellow vegetables of mutagenecity induced by various mutagens and on the growth of human cancer cells. PhD Dissertation. Pusan National University, Busan
  5. Farrel KT. 1985. Spices. Condiments and Seasonings. Van Nostrant Company, New York. p 150-155
  6. Park JR, Park SK, Cho YS, Chun SS. 1994. Purification and characterization of myrosinase in dolsan leaf mustard (Brassica juncea) and changes in myrosinase activity during fermentation of leaf mustard kimchi. Korean J Diet Culture 9: 137-142
  7. Kim SY, Choi EH. 2001. Isolation of lactic acid bacteria from Dongchimi juice and lactic acid fermentation using mixed fruit and vegetable juices. Food Sci Biotechnol 10: 76-83
  8. Cho YS, Ha BS, Park SK, Chun SS. 1993. Contents of carotenoids and chlorophylls in dolsan leaf mustard (Brassica juncea). Korean J Diet Culture 8: 153-157
  9. Song ES, Jean YS, Cheigh HS. 1997. Changes in chlorophylls and carotenoids of mustard leaf kimchi during fermentation and their antioxidative on the lipid peroxidation. J Korean Soc Food Sci Nutr 26: 563-568
  10. Hill CB, William PH, Carlson DG, Tookey HL. 1987. Variation in glucosinolates in oriental Brassica vegetables. J Am Soc Hort Sci 112: 309-313
  11. Kim YT. 2003. Standard preparation of cancer preventive mustard leaf kimchi and its anticancer effects. PhD Dissertation. Pusan National University, Busan
  12. Matsushima T, Sugiura T, Nagao M, Yahagi T, Shirai A, Sawamura M. 1980. Factors modulation mutagenicity in microbial test. In Short-Term Test Systmes for Detecting Carcinogenesis. Norpoth KH, Garner RD, eds. Springer, Gerling. p 273
  13. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicy test. Mutat Res 113: 173-215
  14. Park JG, Frucht H, LaRocca RV, Bliss DP, Kurita Y, Chen TR, Henslee JG, Trepel JB, Jensen RT, Johnson BE, Bang YJ, Kim JP, Gazdar AF. 1990. Characterization of cell lines established from human gastric carcinoma. Cancer Res 50: 2773-2780
  15. Choi YH, Baek JH, Yoo M, Chung H, Kim ND, Kim KW. 2000. Induction of apoptosis by ursolic acid though activation of caspases and down-regulation of c-IAPs in human prostate epithelial cells. Int J Oncol 17: 565-571
  16. Park KY, Baek KA, Rhee SH, Cheigh HS. 1995. Antimutagenic effect of kimchi. Foods Biotech 4: 141-145
  17. Cho EJ. 1999. Standardization and cancer chemopreventive activities of chinese cabbage kimchi. PhD Dissertation. Pusan National University, Busan
  18. Kim HY. 2002. Antioxidative effects of mustard leaves in vitro and in vivo. PhD Dissertation. Pusan National University, Busan
  19. Kang SG. 1995. Structural analysis of major antimi-crobial substance obtained from leaf mustard (Brassica juncea). J Korean Soc Food Nutr 24: 702-706

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