단백질 칩 기판의 표면 스크래칭 효과

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현준원;황정일
Hyun, June-Won;Hwang, Jeong-Il

  • 발행 : 2007.04.30

초록

[ $NiCl_2$ ] and poly-L-lysine coated protein chip plates have been fabricated using a spin coating system. Water has been used as solvent and scratching effects on glass slides and ITO have been investigated. We also observed the surface properties of $NiCl_2$ and poly-L-lysine coated slides by using PSA(Particle size analyzer) and AFM(Atomic force microscope). The AFM results imply that the surface patterns created in the spin coating system determine the protein adsorption. Adsorption of histidine-tagged KRS proteins immobilized on glass slides and ITO was analyzed using a BAS image system. The results suggest that the scratching effect was increased ability of protein adsorption.

키워드

$NiCl_2$ and poly-L-lysine;Protein chip plate;Spin coating;Scratching effect;Protein adsorption

참고문헌

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