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Study on Tumor Necrosis Factor-α· Gene Polymorphism in Rheumatoid Arthritis

류마티스 관절염에 있어 종양괴사인자 다형성에 대한 연구

  • Kim, Kyung-Un (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dageu Haany University) ;
  • Lee, Kyung-Min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dageu Haany University) ;
  • Lee, Bong-Hyo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dageu Haany University) ;
  • Lim, Seong-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dageu Haany University) ;
  • Jung, Tae-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dageu Haany University) ;
  • Seo, Jung-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dageu Haany University)
  • 김경운 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이경민 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이봉효 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 정태영 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 서정철 (대구한의대학교 한의과대학 침구경혈학교실)
  • Published : 2007.06.30

Abstract

Objectives : Tumor necrosis factor-${\alpha}{\cdot}$(TNF-${\alpha}{\cdot}$) is a proinflammatory cytokine involved in the pathogenesis of rheumatoid arthritis. This study was designed to investigate the relation between TNF-${\alpha}{\cdot}$ gene polymorphism and rheumatoid arthritis in Korean population. Methods : This study was carried out on 103 rheumatoid arthritis patients who fulfilled the American College of Rheumatology 1987 revised criteria for rheumatoid arthritis and 208 healthy control subjects. Blood samples from all subjects were obtained for DNA extraction. The extracted DNA was amplified by polymerse chain reaction(PCR). PCR products were visualized by 2% agarose gel electrophoresis. We investigated the genotyping of TNF-${\alpha}{\cdot}$ by using Pyrosequencing. Results : The genotypes of TNF-${\alpha}{\cdot}$ gene were GG, AG and AA. While the distribution of TNF-${\alpha}{\cdot}$ polymorphism in control subjects was 92.31%, 7.21%, 0.48% respectively, in rheumatoid arthritis patients was 93.20%, 6.80%, 0.00%(GG, AG. AA). There was no statistical significant allelic frequency difference between control and rheumatoid arthritis groups. Conclusion : We concluded that there was no significant association between TNF-${\alpha}{\cdot}$ gene polymorphism and rheumatoid arthritis. However, the findings of this study need to be confirmed in more patients and further studies.

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