Occurrence of hydropericardium-hepatitis syndrome in broilers and baeksemis raised in Korea

  • Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University) ;
  • Ha, Jong-Su (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Jin-Hyun (College of Veterinary Medicine, Kyungpook National University)
  • Accepted : 2007.08.30
  • Published : 2007.09.30


The occurrence of hydropericardium-hepatitis syndrome was confirmed for the first time in Korea from chickens submitted for diagnosis to our laboratory from broiler and baeksemi farms. Clinical signs included depression, inappetence, ruffled feathers and an increase in mortality. At necropsy, severe hydropericardium and hepatic necrosis was characteristically found. The most remarkable microscopic changes were seen in the liver, including basophilic intranuclear inclusion bodies in the hepatocytes, massive hemorrhages and necrosis in the liver parenchyma. Based on polymerase chain reaction and transmission electron microscopy (TEM), we could identify the fowl adenoviruses as the causative agent of the disease. In the TEM, we observed the presence of a large number of intranuclear virus particles in the hepatocytes. We could also find the PCR amplification of 700 bp DNA from purified hydropericardium-hepatitis syndrome viral DNA.


  1. Abdul-Aziz TA, Hasan SY. Hydropericardium syndrome in broiler chickens: its contagious nature and pathology. Res Vet Sci 1995, 59, 219-221 https://doi.org/10.1016/0034-5288(95)90005-5
  2. Afzal M, Ahmad I. Efficacy of an inactivated vaccine against hydropericardium syndrome in broilers. Vet Rec 1990, 126, 59-60
  3. Afzal M, Muneer R, Stein G. Studies on the aetiology of hydropericardium syndrome (Angara disease) in broilers. Vet Rec 1991, 128, 591-593 https://doi.org/10.1136/vr.128.25.591
  4. Akhtar S, Zahid S, Khan MI. Risk factors associated with hydropericardium syndrome in broiler flocks. Vet Rec 1992, 131, 481-484 https://doi.org/10.1136/vr.131.21.481
  5. Anjum AD. Experimental transmission of hydropericardium syndrome and protection against it in commercial broiler chickens. Avian Pathol 1990, 19, 655-660 https://doi.org/10.1080/03079459008418721
  6. Anjum AD, Sabri MA, Iqbal Z. Hydropericarditis syndrome in broiler chickens in Pakistan. Vet Rec 1989, 124, 247-248 https://doi.org/10.1136/vr.124.10.247
  7. Asrani RK, Gupta VK, Sharma SK, Singh SP, Katoch RC. Hydropericardium-hepatopathy syndrome in Asian poultry. Vet Rec 1997, 141, 271-273 https://doi.org/10.1136/vr.141.11.271
  8. Balamurugan V, Kataria JM. The Hydropericardium syndrome in poultry-a current scenario. Vet Res Commun 2004, 28, 127-148
  9. Barrow GI, Feltham RKA. Cowan and Steel's Manual for the Identification of Medical Bacteria. 3rd ed. Cambridge univ Press, New York, 2004
  10. Biswas PK, Sil BK, Faruque R, Ahmed S, Biswas D, Chowdhury S. Adenovirus induced hydropericardium-hepatitis Syndrome in Broiler Parent Chickens in Chittagong, Bangladesh. Pak J Biol Sci 2002, 5, 994-996 https://doi.org/10.3923/pjbs.2002.994.996
  11. Chandra R, Shukla SK. Kumar M. The Hydropericardium syndrome and inclusion body hepatitis in domestic fowl. Trop Anim Health Prod 2000, 32, 99-111 https://doi.org/10.1023/A:1005230703093
  12. Chandra R, Shulda SK, Kumar M, Garg SK. Electron microscopic demonstration of an adenovirus in the hepatocytes of birds experimentally infected with hydropericardium syndrome. Vet Rec 1997, 140, 70-71
  13. Ding Y, Wang H, Shen H, Li Z, Geng J, Han H, Cai J, Li X, Kang W, Weng D, Lu Y, Wu D, He L, Yao K. The clinical pathology of severe acute: respiratory syndrome (SARS): a report from China. J Pathol. 2003, 200, 282-289 https://doi.org/10.1002/path.1440
  14. Ganesh K, Raghavan R. Hydropericardium hepatitis syndrome of broiler poultry: current status of research. Res Vet Sci 2000, 68, 201-206 https://doi.org/10.1053/rvsc.1999.0365
  15. Ganesh K, Raghavan R, Gowda RNS, Satyanarayana ML, Suryanarayana VVS. Purification and characterization of the aetiological agent of hydropericardium hepatitis syndrome from infected liver tissues of broiler chickens. Trop Anim Health Prod 2002, 34, 7-17 https://doi.org/10.1023/A:1013777509538
  16. Ganesh K, Suryanarayana VVS, Raghavan R. Detection of fowl adenovirus associated with hydropericardium hepatitis syndrome by a polymerase chain reaction. Vet Res Commun 2002, 26, 73-80 https://doi.org/10.1023/A:1013361906791
  17. Ganesh K, Suryanarayana VVS, Raghavan R, Gowda S. Nucleotide sequence of L1 and part of P1 of hexon gene of fowl adenovirus associated with hydropericardium hepatitis syndrome differs with the corresponding region of other fowl adenoviruses. Vet Microbiol 2001, 78, 1-11 https://doi.org/10.1016/S0378-1135(00)00288-1
  18. Hess M. Detection and differentiation of avian adenoviruses: a review. Avian Pathol 2000, 29, 195-206 https://doi.org/10.1080/03079450050045440
  19. Hess M, Raue R, Prusas C. Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium. Avian Pathol 1999, 28, 433-439 https://doi.org/10.1080/03079459994443
  20. Kim KS, Kim SB, Rhee YO, Choi CO, Namgoong S, Park KS. Occurrence of inclusion body hepatitis in chickens raised in Korea. Korean J Vet Res 1981, 21, 41-43
  21. Mazaheri A, Prusas C, Vo, M, Hess M. Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens. Avian Pathol 1998, 27, 269-276 https://doi.org/10.1080/03079459808419335
  22. Meulemans G, Boschmans M, Van den Berg TP, Decaesstecker M. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathol 2001, 30, 655-660 https://doi.org/10.1080/03079450120092143
  23. Naeem K, Niazi T, Malik SA, Cheema AH. Immunosuppressive potential and pathogenicity of an avian adenovirus isolate involved in hydropericardium syndrome in broilers. Avian Dis 1995, 39, 723-728 https://doi.org/10.2307/1592408
  24. Naeem K, Akram HS. Hydropericardium syndrome outbreak in a pigeon flock. Vet Rec 1995, 136, 296-297 https://doi.org/10.1136/vr.136.12.296
  25. Nakamura K, Shoyama T, Mase M, Imada T, Yamada M. Reproduction of hydropericardium syndrome in three-week-old cyclophosphamide-treated specific-pathogen-free chickens by adenoviruses from inclusion body hepatitis. Avian Dis 2003, 47,169-174 https://doi.org/10.1637/0005-2086(2003)047[0169:ROHSIT]2.0.CO;2
  26. Nakamura K, Tanaka H, Masl M, Imada T, Yamada M. Pancreatic necrosis and ventricular erosion in adenovirus-associated hydropericardium syndrome of broilers. Vet Pathol 2002, 39, 403-406 https://doi.org/10.1354/vp.39-3-403
  27. Roy P, Vairamuthu S, Sakthivelan SM, Purushothaman V. Hydropericardium syndrome in Japanese quail (Coturnix coturnix japonica). Vet Rec 2004, 155, 273-274 https://doi.org/10.1136/vr.155.9.273
  28. Shane S.M. The changing face of poultry disease: a twenty-year retrospective. World poultry 2004, 20, 268
  29. Tom H, Gonzlez C, Cerda L, Morales MA, Dooner P, Salamero M. Prevention of Inclusion body hepatitis/ hydropericardium syndrome in progeny chickens by vaccination of breeders with fowl adenovirus and chicken anemia virus. Avian Dis 2002, 46, 547-554 https://doi.org/10.1637/0005-2086(2002)046[0547:POIBHH]2.0.CO;2