Isolation of formaldehyde-responsive proteins in Arabidopsis

Formaldehyde에 반응하는 애기장대 단백질의 분리

  • Kwon, Mi (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Park, Hyun Jin (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Seo, Jae Hyun (Department of Forest Products, College of Forest Science, Kookmin University)
  • 권미 (국민대학교 삼림과학대학 임산공학과) ;
  • 박현진 (국민대학교 삼림과학대학 임산공학과) ;
  • 서재현 (국민대학교 삼림과학대학 임산공학과)
  • Received : 2007.05.10
  • Accepted : 2007.06.27
  • Published : 2007.07.25

Abstract

Plant can detoxify the effect of the volatile organic compounds (VOC) such as formaldehyde and toluene, however, mechanisms of VOC detoxification are largely unknown in plant system. This study was performed to investigate phenotypic changes of Arabidopsis seedlings upon treatment of either formalin or toluene. Formalin treatment up to twenty four hours didn't cause any significant phenotypic damages on the leaf surface of 27 DAG Arabidopsis seedlings. However, the protein profile of formalin-treated seedlings was significantly different from that of mock control. Using automated electrophoresis system, the molecular weight of each formaldehyde-responsive protein (FRP) was predicted and its formaldehyde-dependent expression was confirmed at transcription level by quantitative real-time RT-PCR analysis. Four FRPs isolated in this study are the novel proteins with unknown functions but highly homologous to the stress-related proteins.

Acknowledgement

Supported by : 학술진흥재단

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