Fermented Peel of Citrus sunki Hort. ex Tanaka Promotes Ethanol Metabolism and Suppresses Body Fat Accumulation

  • Cui, Zhi-Gang (Departmant of Medicine, Cheju National University) ;
  • Kim, Bo-Youn (Departmant of Medicine, Cheju National University) ;
  • Kang, Shin-Hae (Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Young-Jae (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Dae-Ho (Departmant of Medicine, Cheju National University) ;
  • Lee, Young-Ki (Departmant of Medicine, Cheju National University) ;
  • Park, Deok-Bae (Departmant of Medicine, Cheju National University)
  • Published : 2007.04.30


Citrus sunki Hort. ex Tanaka is one of several Jeju-native citrus fruits. A number of biological properties for citrus fruits have been suggested, however little is known about those of C. sunki. The present study tested whether fermented product of C. sunki peel (FSP) might affect the activities of enzymes regulating ethanol metabolism. Effects on body weight gain as well as on fatty liver formation were also investigated. The activities of alcohol dehydrogenase and aldehyde dehydrogenase were stimulated remarkably by FSP. Excessive ethanol-induced cytotoxicity was also prevented by FSP in HepG2 cells. FSP decreased the weight gain and fatty liver formation induced by a high-fat diet in mice. From these results, FSP might be a potent source of neutraceuticals useful for preventing ethanol-induced health problems.


  1. Kim HY. Distribution, taxonomy, horticultural characters of the local Citrus spp. in Cheju, and the genetic markers among them. PhD thesis, Cheonnam National University, Jeonnam, Korea (1988)
  2. Kawamori T, Tanaka T, Hirose M, Ohnishi M, Mori H. Inhibitory effects of D-limonene on the development of colonic aberrant crypt foci induced by azoxymethane in F344 rats. Carcinogenesis 17: 369-372 (1996) https://doi.org/10.1093/carcin/17.2.369
  3. Murakami A, Nakamura Y, Tanaka T, Kawabata K, Takahashi D, Koshimizu K, Ohigashi H. Suppression by Citrus auraptene of phorbol ester- and endotoxin-induced inflammatory responses: role of attenuation of leukocyte activation. Carcinogenesis 21: 1843-1850 (2000) https://doi.org/10.1093/carcin/21.10.1843
  4. Kang SH, Lee YJ, Lee CH, Kim SJ, Lee DH, Lee YK, Park DB. Physiological activities of peel of Jeju-indigenous Citrus sunki Hort. Tanaka. Korean J. Food Sci. Technol. 37: 983-988 (2005)
  5. Moon SW, Kang SH, Jin YJ, Park JK, Lee YD, Lee YK, Park DB, Kim SJ. Fermentation of Citrus unshiu Marc. and functional characteristics of the fermented products. Korean J. Food Sci. Technol. 36: 669-676 (2004)
  6. Bostian KA, Betts GF. Kinetics and reaction mechanism of potassium-activated aldehyde dehydrogenase from Saccharomyces cerevisiae. Biochem. J. 173: 787-798 (1978) https://doi.org/10.1042/bj1730787
  7. Shin DB, Lee DW, Yang R, Kim JA. Antioxidative properties and flavonoids contents of matured Citrus peel extracts. Food Sci. Biotechnol. 15: 357-362 (2006)
  8. Jeong WS, Park SW, Chung SK. The antioxidative activity of Korean Citrus unshiu peels. Food Sci. Biotechnol. 6: 292-296 (1997)
  9. Lim HK, Yoo ES, Moon JY, Jeon YJ, Cho SK. Antioxidant activity of extracts from dangyuja (Citrus grandis Osbeck) fruits produced in Jeju island. Food Sci. Biotechnol. 15: 312-316 (2006)
  10. Tanaka T, Makita H, Ohnishi M, Hirose Y, Wang A, Mori H, Satoh K, Hara A, Ogawa H. Chemoprevention of 4-nitroquinolinel-oxideinduced oral carcinogenesis by dietary curcumin and hesperidin: comparison with the protective effect of $\beta$-carotene. Cancer Res. 54: 4653-4659 (1994)
  11. Tanaka J. Mori H. Inhibition of colon carcinogenesis by nonnutritive constituents in foods. J. Toxicol. Pathol. 9: 139-149 (1996) https://doi.org/10.1293/tox.9.139
  12. Kim YC, Koh KS, Koh JS. Changes of flavonoids in the peel of Jeju native citrus fruits during maturation. Food Sci. Biotechnol. 10: 483-487 (2001)
  13. Lio M, Masaru M, Iwanami T, Yoshikatsu Y. Flavonoids as a possible preventive of dental carries. Agr. Biol. Chem. Tokyo 8: 2143-2145 (1984)
  14. Park KJ, Lee MJ, Kang H, Lee SH, Cho I, Lee HH. Saeng-MaekSan, a medicinal complex, protects liver cell damage induced by alcohol. Biol. Pharm. Bull. 25: 1451-1455 (2002) https://doi.org/10.1248/bpb.25.1451
  15. Lee CH, Jeong TS, Choi YK, Hyun BH, Oh GT, Kim EH, Kim JR, Han JI, Bok SH. Anti-artherogenic effect of citrus flavonoids, naringin, and naringenin, associated with hepatic ACAT and aortic VCAM-1 and MCP-1 in high cholesterol-fed rabbits. Biochem. Bioph. Res. Co. 284: 681-688 (2001) https://doi.org/10.1006/bbrc.2001.5001
  16. Cho C, Park BJ, Chung SH, Kim CB, Cha BS, Byun MW. Antibacterial and anti-fungal activity of citrus (Citrus unchiu) essential oil extracted from peel by-products. Food Sci. Biotechnol. 13: 384-386 (2004)
  17. Lam LKT, Zhang J, Hasegawa S, Schut HAJ. Inhibition of chemically induced carcinogenesis by citrus limonoids. Vol. 546, pp. 209-219. In: Food Phytochemicals for Cancer Prevention I. Huang MT, Osawa T, Ho CT, Rosen RT (eds). American Chemical Society, ACS Symposium Series, Washington, DC, USA (1994)
  18. Neuman MG, Koren G, Tiribelli C. In vitro, assessment of the ethanol-induced hepatotoxicity on HepG2 cell line. Biochem. Bioph. Res. Co. 197: 931-942 (1993)
  19. Bok SH, Shin YW, Bae KH, Jeong TS, Kwon YK, Park YB, Choi MS. Effects of naringin and lovastatin on plasma and hepatic lipids in high-fat and high-cholesterol fed rats. Nutr. Res. 20: 1007-1015 (2000) https://doi.org/10.1016/S0271-5317(00)00191-3
  20. Neuman MG, Shear NH, Cameron RG, Katz G, Tiribelli C. Ethanolinduced apoptosis in vitro. Clin. Biochem. 32: 547-555 (1999) https://doi.org/10.1016/S0009-9120(99)00054-5
  21. Parrizas M, Saltiel AR, LeRoith D. Insulin-like growth factor 1 inhibits apoptosis using the phosphatidylinositol 3'-kinase and mitogen-activated protein kinase pathways. J. Biol. Chem. 272: 154-161 (1997) https://doi.org/10.1074/jbc.272.1.154