Hypoglycemic Effects of Basidiomycetes Mycelia and Cereals Fermented with Basidiomycetes

버섯 균사체 및 배양곡물의 혈당강하 효과

  • Published : 2007.10.30


To develop basidiomycetes-fermented cereals with hypoglycemic property, inhibitory effects of basidiomycetes mycelia and basidiomycetes-fermented cereals on postprandial glucose were investigated. In vitro effect of basidiomycetes mycelia on retarding the membrane transport of glucose was compared with pectin. For basidiomycetes mycelia, $13.1{\pm}3.6{\sim}41.8{\pm}8.0%$ of total glucose remained in inner solution of dialysis membrane after dialysis for 120 min, indicating that most of basidiomycetes mycelia might effectively retard membrane transport of glucose. Glucose tolerance of basidiomycetes mycelia and basidiomycetes-fermented cereals was tested on streptozotocin-induced diabetic rats administrated with maltose. Postprandial glucose levels of basidiomycetes mycelia, $389.4{\pm}43.8{\sim}426.3{\pm}49.4mg/dL$, were considerably lower than that of control, $535.3{\pm}78.6mg/dL$, at 30 minutes after maltose administration. Namely, basidiomycetes mycelia showed better postprandial glucose lowering effect than pectin. Brown rice and barley fermented with Paecilomyces japonica showed much lower postprandial glucose level than raw brown rice and barley, especially hypoglycemic effect of barley fermented with Paecilomyces japonica was significant.


  1. Strowig S, Raskin P. 1992. Glycemic control and diabetic complication. Diabetes Care 15: 1126-1140
  2. Adverse events and their association with treatment regimens in the diabetes control and complications trial. 1995. Diabetes Care 18: 1415-1427
  3. Torsdottir I, Alpsten M, Andersson H, Einasson S. 1989. Dietary guar gum effects on postprandial blood glucose, insulin and hydroxyproline in humans. J Nutr 119: 1925-1931
  4. Blackburn NA, Redfern JS, Jaris H, Holgate AM, Hanning I, Scarpello JHB, Johnson IT, Read NW. 1984. The mechanism of action of guar gum in improving glucose tolerance in man. Clin Sci 66: 329-336
  5. Puls HP, Krause L, Muller H, Schutt R, Thomas G. 1984. Inhibitors of the rate of carbohydrate and lipid absorption by the intestine. Int J Obesity 8: 181-190
  6. Lee YR, Nam SH, Kang MY. 2006. Hypoglycemic effect of the giant embryonic rice supplementation on streptozotocin-induced diabetic rats. Korean J Food Sci Technol 38: 427-431
  7. Kim YY, Cho RW, Chung SH, Koo SJ. 1999. Anti-hyperglycemic effect of Cortex Mori radicis in db/db mice. Korean J Food Sci Technol 31: 1057-1064
  8. Ortiz-Andrade RR, Garcia-Jimenez S, Castillo-Espana P, Ramirez-Avila G, Villalobos-Molina R, Estrada-Soto S. 2007. $\alpha$-Glucosidase inhibitory activity of the methanolic extract from Tournefortia hartwegiana: an anti-hyperglycemic agent. J Ethnopharmacol 109: 48-53
  9. Chihara G, Hamuro H, Maeda Y, Arai Y, Fukuoka K. 1970. Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan from Lentinus edodes. Cancer Res 30: 2776-2781
  10. Nagata J, Higashiuesato Y, Maeda G, Chinen I, Saito M, Iwabuchi K, Onoe K. 2001. Effects of water-soluble hemicellulose from soybean hull on serum antibody levels and activation of macrophages in rats. J Agric Food Chem 49: 4965-4970
  11. Watanabe T, Yamada T, Tanaka H, Jiang S, Mazumder TK, Nagai S, Tsuji K. 2002. Antihypertensive effect of gamma-aminobutyric acid-enriched Agaricus blazei on spontaneously hypertensive rats. J Jpn Soc Food Sci Technol 49: 166-173
  12. Sumi H, Yatagai C, Matsubara K. 1996. Anti-platelet aggregation and plasma fibrinolysis accelerating activities in mushroom extracts (Pleurotus ostreatus and Lentinus edodes). J Jpn Soc Food Sci Technol 43: 318-321
  13. Yuan Z, He P, Cui J, Takeuchi H. 1998. Hypoglycemic effect of water-soluble polysaccharide from Auricularia-judae Quel. on genetically diabetic KK-A mice. Biosci Biotechnol Biochem 62: 1898-1903
  14. Kiho T, Sobue S, Ukai S. 1994. Structural features and hypoglycemic activities of two polysaccharides from a hot-water extract of Agrocybe cylindracea. Carbohydr Res 251: 81-87
  15. Kato Y. 1993. Influence of water-insoluble dietary fiber on in vitro glucose diffusion speed. J Jpn Soc Nutr Food Sci 46: 351-355
  16. Lee YT. 2003. Preparation and hypoglycemic effect of reconstituted grain added with selected medicinal herb extract. Korean J Food Sci Technol 35: 527-531
  17. Rainbird AL, Low AG, Zebrowska T. 1984. Effect of guar gum on glucose and water absorption from isolated loops of jejunum in conscious growing pigs. Brit J Nutr 52: 489-498
  18. Kato Y, Akiyama M. 1993. Gel-filtration chromatography of dextrans and maltodextrins on a water-insoluble dietary fiber column. J Jpn Soc Nutr Food Sci 46: 161-166
  19. Huang SJ, Tsai SY, Lee YL, Mau JL. 2006. Nonvolatile taste components of fruit bodies and mycelia of Cordyceps militaris. Lebensm Wiss Technol 39: 577-583
  20. Tsai SY, Weng CC, Huang SJ, Chen CC, Mau JL. 2006. Nonvolatile taste components of Grifola frondosa, Morchella esculenta and Termitomyces albuminosus mycelia. Lebensm Wiss Technol 39: 1066-1071
  21. Mizuno T. 1995. Bioactive biomolecules of mushrooms: Food function and medicinal effect of mushroom fungi. Food Rev Int 11: 7-21
  22. Kiho T, Ookubo K, Usui S, Ukai S, Hirano K. 1999. Structural features and hypoglycemic activity of a polysaccharide (CS-F10) from the cultured mycelium of Cordyceps sinensis. Biol Pharm Bull 22: 966-970
  23. Hwang HJ, Kim SW, Lim JM, Joo JH, Kim HO, Kim HM, Yun JW. 2005. Hypoglycemic effects of crude exopolysaccharides produced by a medicinal mushroom Phellinus baumii in streptozotocin-induced diabetic rats. Life Sci 76: 3069-3080
  24. Anderson JW. 1985. Physiological and metabolic effects of diatary fiber. Fed Proc 44: 2902-2906
  25. Wolever TMS. 1990. Relationship between dietary fiber content and composition in foods and the glycemic index. Am J Clin Nutr 51: 72-75
  26. Battilana P, Ornstein K, Minehira K, Schwarz JM, Acheson K, Schneiter P, Burri J, Jequier E, Tappy L. 2001. Mechanisms of action of $\beta$-glucan in postprandial glucose metabolism in healthy men. Eur J Clin Nutr 55: 327-333
  27. Delorme S, Chiasson JL. 2005. Acarbose in the prevention of cardiovascular disease in subjects with impaired glucose tolerance and type 2 diabetes mellitus. Current Opinion Pharmacol 5: 184-189
  28. Honda M, Hara Y. 1993. Inhibition of rat small intestinal sucrase and $\alpha$-glucosidase activities by tea polyphenols. Biosci Biotech Biochem 57: 123-128
  29. Kim SD, Nho HJ. 2004. Isolation and characterization of $\alpha$- glucosidase inhibitor from the Fungus Ganoderma lucidum. J Microbiol 42: 223-227
  30. Kim DH, Choi HJ, Bae EA, Han MJ, Park SY. 1998. Effect of artificially cultured Phellinus linteus on harmful intestinal bacterial enzymes and rat intestinal $\alpha$-glucosidases. J Food Hygiene Safety 13: 20-23
  31. Choi HD, Park YK, Choi IW, Kim HM, Kim SR. 2003. Development of basidiomycetes-fermented cereals with hypoglycemic and anti-dementia property and their products. KFRI report GA0398-0255
  32. Lee DS, Lee SH. 2001. Genistein, a soy isoflavone, is a potent alpha-glucosidase inhibitor. FEBS Letters 50: 84-86
  33. Randhir R, Shetty K. 2007. Mung beans processed by solid-state bioconversion improves phenolic content and functionality relevant for diabetes and ulcer management. Inovative Food Sci Emerging Technol 8: 197-207
  34. McCue P, Horii A, Shetty K. 2004. Mobilization of phenolic antioxidants from defatted soybean powders by Lentinus edodes during solid-state bioprocessing is associated with enhanced production of laccase. Inovative Food Sci Emerging Technol 5: 385-392

Cited by

  1. Physiological Activities of Extracts from Fomitella fraxinea on Brown Rice added Rice Bran, Pine Needle and Tumeric Powder vol.39, pp.2, 2011,
  2. Physiological Activities of Extracts from Phellinus linteus on Brown Rice added Rice Bran, Pine Needle and Tumeric Powder vol.39, pp.2, 2011,
  3. Inhibition of α-Glucosidase by a Semi-Purified Ethyl Acetate Fraction from Submerged-Liquid Culture of Agaricus blazei Murill vol.21, pp.11, 2011,
  4. Physicochemical components of Astragalus membranaceus fermented with mushroom mycelia vol.23, pp.1, 2016,
  5. Changes of Antioxidant Activities on Cultured Ginseng with Mushroom Mycelia During Cultivation vol.39, pp.9, 2010,