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Ethanol Induced Leucocytic and Hepatic DNA Strand Breaks Are Prevented by Styela clava and Styela plicata Supplementation in Male SD Rats

알코올로 인한 흰쥐의 백혈구 및 간 DNA 손상에 미치는 미더덕과 오만둥이 분말의 보충섭취 효과

  • Kim, Jung-Mi (Dept. of Food and Nutrition, Kyungnam University) ;
  • Park, Hae-Ryoung (Dept. of Food Science and Biotechology, Kyungnam University) ;
  • Lee, Seung-Cheol (Dept. of Food Science and Biotechology, Kyungnam University) ;
  • Park, Eun-Ju (Dept. of Food and Nutrition, Kyungnam University)
  • 김정미 (경남대학교 식품영양학과) ;
  • 박해룡 (경남대학교 식품생명학과) ;
  • 이승철 (경남대학교 식품생명학과) ;
  • 박은주 (경남대학교 식품영양학과)
  • Published : 2007.10.30

Abstract

In this study, the ability of Styela clava or Styela plicata to reduce ethanol-induced hepatotoxicity and hepatic and leucocytic DNA damages was evaluated. Twenty four male SD rats were given 25% ethanol containing water (ad lib, p.o.) and divided into 3 groups; ethanol treated control group (EtOH), ethano1+3% S. clava (EtOH+SC), and ethano1+3% S. plicata (EtOH+SP). After 6 weeks, the supplementation of S. clava reduced the plasma ALT, ALP and LDH activities significantly (p<0.05), while S. plicata induced significant decrease in the plasma LDH activity only. The comet assay was employed to quantify the alcohol-induced DNA damage in rat hepatocytes and leucocytes. A significant protective effect on hepatic and leucocytic DNA damages was observed in S. clava or S. plicata supplemented groups compared to the EtOH control group. The hepatic DNA damage was correlated positively with plasma ALP and LDH activities. These results demonstrated that S. clava or S. plicata supplementation protected alcohol-induced hepatic and leucocytic DNA damage.

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