Monitoring on Functional Properties of Solid Cheonggukjang Extracts by Using Response Surface Methodology

반응표면분석을 이용한 생청국장 추출물의 기능성에 대한 모니터링

  • Published : 2007.10.30


Extraction characteristics of solid Cheonggukjang and the functional properties of corresponding extracts were monitored by the response surface methodology (RSM). The maximum extraction yield of 12.08% was obtained under the specific extraction conditions, such as microwave power of 132.40 W, ratio of solvent to sample content of 7.57 g/mL and extraction time of 14.11 min. The maximum electron donating ability (EDA) was found as 33.53% at the conditions of 113.26 W microwave power, 16.39 g/mL of ratio of solvent to sample content and 12.67 min extraction time. The maximum superoxide dismutase (SOD) -like activity was 89.62% under the extraction conditions of 61.82 W, 17.06 g/mL and 9.03 min. Based on superimposition of three dimensional RSM with respect to extraction yield, electron donating ability and SOD-like activity obtained under the various extraction conditions, the optimum ranges of extraction conditions were found to be microwave power of $75{\sim}140W$, ratio of solvent to sample content of $3{\sim}12g/mL$ and extraction time of $1{\sim}11min$, respectively.


  1. Kim DH, Lee GY, Kim NM, Lee JS. 2003. Physiological functionality of various extracts from danmemil and legumes. Korean J Food Nutr 16: 347-352
  2. Jung YK, Lee K, No HK, Kim SD. 2006. Effect of sea tangle on fermentation and quality characteristics of Cheongbukjang. Korean J Food Preserv 13: 95-101
  3. Hong SW, Kim JY, Lee BK, Chung KS. 2006. The bacterial biological response modifier enriched Chungkookjang fermentation. Korean Food Sci Technol 38: 548-553
  4. Shon MY, Kwon SH, Park SK, Park JR, Choi JS. 2001. Changes in chemical components of black bean Chungkugjang added with kiwi and radish during fermentation. Korean J Postharvest Sci Technol 8: 449-455
  5. Yoo CK, Seo WS, Lee CS, Kang SM. 1998. Purification and characterization of fibrinolytic enzyme excreted by Bacillus subtilis K-54 isolated from Chunggukjang. Korean J Appl Microbiol Biotechnol 26: 507-514
  6. Hosoi T. 1996. Recent progress in treatment of osteoporosis. Nippon Ronen Igakkai Zasshi 33: 240-244
  7. Ko HS, Cho DH, Hwang SY, Kim YM. 1999. The effect of quality improvement by Chungkukjang's processing methods. Korean J Food Nutr 12: 1-6
  8. Giese J. 1992. Advances in microwave food processing. Food Technol 46: 118-123
  9. Schiffmann RF. 1992. Microwave processing in the U.S. food industry. Food Technol 46: 50-56
  10. Contard N, Guilbert S, Cuq JL. 1992. Edible wheat gluten films: influence of the main process variables on film properties using response surface methodology. J Food Sci 57: 190-196
  11. Lee GD, Lee JE, Kwon JH. 2000. Application of response surface methodology in food industry. Food Ind 33: 33-45
  12. SAS Institute, Inc. 1990. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA
  13. AOAC. 1995. Official Methods of Analysis. 17th ed. Method 3.1.03. Association of Official Analytical Chemists, Washington DC, USA
  14. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 232-239
  15. Gray JI, Dugan Jr LR. 1975. Inhibition of N-nitrosamine formation in model food system. J Food Sci 40: 981-984
  16. Wong TC, Luh BS, Whitaker JR. 1971. Isolation and characterization of polyphenol oxidase of clingstone peach. Plant Physiol 48: 19-23
  17. Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33: 626-632

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  1. Prediction of Optimal Microwave-Assisted Extraction Conditions for Functional Properties from Fluid Cheonggukjang Extracts vol.36, pp.11, 2007,