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Anti-Proliferation Effects of Decursin from Angelica gigas Nakai in the MCF-7 Cells Treated with Environmental Hormones

환경호르몬에 의해 유도된 인체 유방암세포의 증식에 대한 당귀로부터 분리한 Decursin 억제효과

  • Park, Kyung-Wuk (Dept. of Food Nutrition, Sunchon National University) ;
  • Choi, Sa-Ra (Dept. of Food Nutrition, Sunchon National University) ;
  • Yang, Hee-Sun (Dept. of Food Nutrition, Sunchon National University) ;
  • Cho, Hyun-Wook (Dept. of Biology, Sunchon National University) ;
  • Kang, Kap-Suk (Dept. of Culinary Arts, Busan College of Information Technology) ;
  • Seo, Kwon-Il (Dept. of Food Nutrition, Sunchon National University)
  • 박경욱 (순천대학교 식품영양학과) ;
  • 최사라 (순천대학교 식품영양학과) ;
  • 양희선 (순천대학교 식품영양학과) ;
  • 조현욱 (순천대학교 생명과학) ;
  • 강갑석 (부산정보대학 호텔조리과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2007.07.30

Abstract

Anti-proliferation effects of decursin from Angelica gigas Nakai were investigated in the MCF-7 cells treated with environmental hormones. The proliferation was decreased in a dose-dependent manner at the concentration over 20 ${\mu}g/mL$ in the MCF-7 cells treated with decursin of various concentrations. The environmental hormones such as $17{\beta}$-estradiol and bisphenol increased the growth of MCF-7 cells in the charcoal-treated FBS (cFBS) medium and the proliferation was the highest at 0.1 ${\mu}M$ among the tested hormone concentration. Decursin was predicted to inhibit the proliferation in a dose-dependent fashion at tested concentrations (1, 3, 10 or 30 ${\mu}g/mL$) in the MCF-7 cells added environmental hormones; however, the survival rate of the cells was lower than that of control cells that were not treated with decursin at 30 ${\mu}g/mL$ concentration. The chromatin condensation and apoptotic body were examined in the decursin treated cells cultured with the cFBS medium added environmental hormones. These results suggest that decursin decreased the proliferation through apoptosis in the MCF-7 cells added environmental hormones.

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