Anticariogenic Activity and Glucosyltransferase Inhibitory Effects of Extracts from Pine Needle and Twig

솔잎 및 소나무 가지 추출물의 항충치 활성 및 glucosyltransferase 억제 효과

  • Published : 2007.06.30

Abstract

To investigate anticariogenic effects of extracts from pine needles and twigs, we measured the extracts' antimicrobial activities against Streptococcus mutans, their glucosyltransferase (GTase) inhibitory effects and their stabilities under various conditions of heat and pH. The 70% ethanol extracts of pine needles and twigs showed greater antimicrobial activities against S. mutans than the hot water extracts, and the pine twig extracts showed greater antimicrobial activity than the pine needle extracts. More specifically, the n-hexane fractions of the pine needles and twigs had the greatest effects against S. mutans, and their minimal inhibition concentration (MIC) was 0.6 mg/mL. The results of the extracts' GTase inhibitory effects indicated that the 70% ethanol extracts had greater effects than hot water extracts, and the pine twig extracts showed greater effects than the pine needle extracts. The antimicrobial activity against S. mutans for the pine needle n-hexane traction was greatly reduced as temperature increased, and was also largely affected by changes in pH. These results suggest that extracts from pine needles and twigs have anticariogenic effects and could be used as anticariogenic food additives.

Keywords

anticariogenic effect;pine needle;pine twig;antimicrobial activity;Streptococcus mutans;glucosyltransferase

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