Inhibitory Effects on the Enzymes Involved in the Inflammation by the Ethanol Extracts of Plant Foodstuffs

식물성 일반식품 자원의 에탄올 추출물이 염증 효소계에 미치는 영향

  • Kwon, Eun-Sook (Department of Food and Nutrition, College of Human Ecology, Seoul National University) ;
  • Kim, Il-Rang (Department of Food and Nutrition, College of Human Ecology, Seoul National University) ;
  • Kwon, Hoon-Jeong (Department of Food and Nutrition, College of Human Ecology, Seoul National University, Research Institute of Human Ecology, Seoul National University)
  • 권은숙 (서울대학교 생활과학대학 식품영양학과) ;
  • 김일낭 (서울대학교 생활과학대학 식품영양학과) ;
  • 권훈정 (서울대학교 생활과학대학 식품영양학과, 서울대학교 생활과학연구소)
  • Published : 2007.06.30

Abstract

Inflammation is a complex process resulting from a variety of mechanisms. Combined inhibition of the activities of enzymes involved in the process may therefore be considered more important in anti-inflammatory property of plant extracts than any single contribution. In this study, the inhibitory effects of the ethanol extracts of thirty plant foods on the activities of secretory phospholipase $A_{2}$ ($sPLA_{2}$), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), and 12-lipoxygenase (12-LOX) were examined. Several legumes, mungbean sprout and some leaf vegetables inhibited the activity of $sPLA_2$, upstream enzyme of inflammation pathway. Only soybean sprout and mungbean sprout significantly inhibited 12-LOX activity. Although most of extracts inhibited the activities of both COX-1 and COX-2, water dropwort and amaranth showed selectivity for the inhibition of COX-2 over COX-1. Especially, mungbean showed anti-inflammatory property at both upstream and downstream of inflammation pathway with relatively low $IC_{50}$ values for $sPLA_{2}$ and COX-2 enzymes. Mungbean sprout exhibited inhibitory effects on all enzymes related to early and late inflammation and soybean sprout suppressed 12-LOX and COX-2 simultaneously, although the activities of these plants were showed at relatively high concentration. Therefore, mungbean, mungbean sprout, and soybean sprout appear to exhibit anti-inflammatory effects by combined inhibition of inflammatory enzymes.

Keywords

inflammation;secretory phospholipase $A_{2}$;cyclooxygenase-l;cyclooxygenase-2;12-lipoxygenase

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