Inhibitory Effects on the Enzymes Involved in the Inflammation by the Ethanol Extracts of Plant Foodstuffs

식물성 일반식품 자원의 에탄올 추출물이 염증 효소계에 미치는 영향

  • Kwon, Eun-Sook (Department of Food and Nutrition, College of Human Ecology, Seoul National University) ;
  • Kim, Il-Rang (Department of Food and Nutrition, College of Human Ecology, Seoul National University) ;
  • Kwon, Hoon-Jeong (Department of Food and Nutrition, College of Human Ecology, Seoul National University, Research Institute of Human Ecology, Seoul National University)
  • 권은숙 (서울대학교 생활과학대학 식품영양학과) ;
  • 김일낭 (서울대학교 생활과학대학 식품영양학과) ;
  • 권훈정 (서울대학교 생활과학대학 식품영양학과, 서울대학교 생활과학연구소)
  • Published : 2007.06.30


Inflammation is a complex process resulting from a variety of mechanisms. Combined inhibition of the activities of enzymes involved in the process may therefore be considered more important in anti-inflammatory property of plant extracts than any single contribution. In this study, the inhibitory effects of the ethanol extracts of thirty plant foods on the activities of secretory phospholipase $A_{2}$ ($sPLA_{2}$), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), and 12-lipoxygenase (12-LOX) were examined. Several legumes, mungbean sprout and some leaf vegetables inhibited the activity of $sPLA_2$, upstream enzyme of inflammation pathway. Only soybean sprout and mungbean sprout significantly inhibited 12-LOX activity. Although most of extracts inhibited the activities of both COX-1 and COX-2, water dropwort and amaranth showed selectivity for the inhibition of COX-2 over COX-1. Especially, mungbean showed anti-inflammatory property at both upstream and downstream of inflammation pathway with relatively low $IC_{50}$ values for $sPLA_{2}$ and COX-2 enzymes. Mungbean sprout exhibited inhibitory effects on all enzymes related to early and late inflammation and soybean sprout suppressed 12-LOX and COX-2 simultaneously, although the activities of these plants were showed at relatively high concentration. Therefore, mungbean, mungbean sprout, and soybean sprout appear to exhibit anti-inflammatory effects by combined inhibition of inflammatory enzymes.


inflammation;secretory phospholipase $A_{2}$;cyclooxygenase-l;cyclooxygenase-2;12-lipoxygenase


  1. Li W, Xia J, Sun GY. Cytokine induction of iNOS and sPLA2 in immortalized astrocytes (DITNC): response to genistein and pyrrolidine dithiocarbamate. J. Interf. Cytok. Res. 19: 121-127 (1999)
  2. Sekiya K, Okuda H. Selective inhibition of platelet lipoxygenase by baicalein. Biochem. Bioph. Res. Co. 105: 1090-1095 (1982)
  3. Balsinde J, Balboa MA, Insel PA, Dennis EA. Regulation and inhibition of phospholipase A2. Annu. Rev. Pharmacol. 39: 175-189 (1999)
  4. Laughton MJ, Evans PJ, Moroney MA, Hoult JR, Halliwell B. Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives. Relationship to antioxidant activity and to iron ion-reducing ability. Biochem. Pharmacol. 42: 1673-1681 (1991)
  5. Piomelli D, Greengard P. Lipoxygenase metabolites of arachidonic acid in neuronal transmembrane signaling. Trends Pharmacol. Sci. 11: 367-373 (1990)
  6. Charlier C, Michaux C. Dual inhibition of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) as a new strategy to provide safer non-steroidal anti-inflammatory drugs. Eur. J. Med. Chem. 38: 645-659 (2003)
  7. Baumann J, von Bruchhausen F, Wurm G. Flavonoids and related compounds as inhibition of arachidonic acid peroxidation. Prostaglandins 20: 627-639 (1980)
  8. Gerhauser C, Klimo K, Heiss E, Neumann I, Gamal-Eldeen A, Knauft J. Lin GY, Sitthimonchai S, Frank N. Mechanism-based in vitro screening of potential cancer chemopreventive agents. Mutat. Res. 523-524: 163-172 (2003)
  9. Landolfi R, Mower RL, Steiner M. Modification of platelet function and arachidonic acid metabolism by bioflavonoids. Structureactivity relations. Biochem. Pharmacal. 33: 1525-1530 (1984)
  10. Funk CD. Prostaglandins and leukotrienes; advances in eicosanoid biology. Science 294: 1871-1875 (2001)
  11. Mutoh M, Talcahashi M, Fukuda K. Matsushima-Hibiya Y. Mutoh H, Sugimura T, Wakabayashi K. Suppression of cyclooxygenase-2 promoter-dependent transcriptional activity in colon cancer cells by chemopreventive agents with a resorcin-type structure. Carcinogenesis 21: 959-963 (2000)
  12. Verheij HM, Slotboom AJ, de Haas GH. Structure and function of phospholipase A2. Rev. Physiol. Bioch. P. 91: 91-203 (1981)