In vitro Anti-inflammatory Activity of the Artemisia fukudo Extracts in Murine Macrophage RAW 264.7 Cells

큰비쑥(Artemisia fukudo) 추출물의 murine macrophage RAW 264.7 세포에서 in vitro 항염효과

  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Lee, Jung-A (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Kil-Nam (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Kim, Ji-Young (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute) ;
  • Park, Soo-Yeong (Jeju Biodiversity Research Institute, Jeju Hi-Tech Industry Development Institute)
  • 윤원종 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 이정아 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 김길남 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 김지영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소) ;
  • 박수영 ((재)제주하이테크산업진흥원 제주생물종다양성연구소)
  • Published : 2007.08.31

Abstract

The present study describes the preliminary evaluation of the anti-inflammatory activities of Artemisia fukudo extracts. The 80% ethanol extract of A. fukudo was sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and butanol. In order to effectively screen for anti-inflammatory agents, we first examined the extracts’ inhibitory effects on the production of pro-inflammatory cytokines activated with lipopolysaccharide. Moreover, we examined the inhibitory effects of the A. fukudo extracts on pro-inflammatory factors (NO, iNOS, COX-2, and $PGE_{2}$) in murine macrophage RAW 264.7 cells. The protein levels were determined by immunoblotting. Of the sequential solvent fractions, the n-hexane and dichloromethane fractions inhibited the mRNA expression of pro-inflammatory cytokines (TNF-${\alpha}$, IL-$1{\beta}$, and IL-6), production of NO and $PGE_{2}$, and the protein levels of iNOS and COX-2. These results suggest that A. fukudo may have signifIcant effects on inflammatory factors, and may be a potential anti-inflammatory therapeutic plant.

본 연구는 쑥 추출물의 항염 활성이 prostaglandins 합성의 저해 및 pro-inflammatory cytokine의 억제기전과 관련이 있을 것으로 예상되어짐에 따라, 큰비쑥(A. fukudo)을 대상으로 80% EtOH로 추출하고 추출물을 극성에 따라 용매분획을 실시하여, 큰비쑥 에탄올 추출물 및 용매분획물들이 염증반응의 주체가 되는 대식 세포 계열인 RAW 264.7 세포에서 LPS 로 유도된 TNF-${\alpha}$, IL-$1{\beta}$ 그리고 IL-6와 같은 pro-inflammatory cytokine과 NO의 생성억제효과, 그리고 iNOS와 COX-2의 단백질 발현 억제효과 및 $PGE_{2}$ 생성 억제효과 등을 통해 알아보았다. 대식세포 계열인 RAW 264.7 세포에 LPS로 자극을 주고 큰비쑥 추출물을 처리하여 확인해본 결과, 추출물 및 분획물들이 다소 차이는 있었지만 TNF-${\alpha}$, IL-$1{\beta}$ 그리고 IL-6에서 생성억제 효과를 나타났다. 또한 헥산, 디클로로메탄 및 에틸아세테이트 분획물에서 NO의 생성억제 효과가 강하게 나타났으며, 헥산과 디클로로메탄 분획물에서는 iNOS, COX-2 및 $PGE_{2}$ 생성 억제 효과가 다른 분획물에 비해 강하게 나타났다. 이러한 결과는 큰비쑥에서 유효성분 추출을 통한 항염증 물질의 연구 또는 예방하거나 치료할 수 있는 염증 억제 성분의 분리 및 그 작용기전 연구에 중요한 기초 자료가 될 것이라 사료된다. 또한 큰비쑥 추출물로부터 염증억제 성분을 도출하고자 활성분획인 헥산과 디클로로메탄 분획물에 대하여 활성성분의 분리가 진행 중이다.

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