Inhibitory Effect of Extracts from Woody Plants on Tumor Necrosis $Factor-{\alpha}$ Production in Lipopolysaccharide-Stimulated RAW264.7 cells

한국산 자생 수목 유래 수피추출물의 종양괴사인자 억제효과

  • Cho, Jae-Youl (R&D Center, Daewoong Pharm. Co., School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 조재열 (대웅제약 중앙연구소, 강원대학교 BT학부대학 생명공학부)
  • Published : 2007.08.30

Abstract

Naturally occurring substances are important biomedical resources with low toxicity and ethnopharmacology-based efficacy. Four out of 45 extracts (Celastrus orbiculatus, Cercis chinensis, Stephanadra incisa, and Weigela subsessilis) prepared from the bark of Korea Forest plants exhibited more than 50% of inhibition on $TNF-{\alpha}$ production in lipopolysaccharide (LPS)-activated RAW264.7 cells at $100\;{\mu}g/m{\ell}$. In particular, potential inhibitory components of 4 extracts showed more than 50% inhibition seemed to be concentrated in methylene chloride (MC) fraction from C. orbiculatus, in ethyl acetate (EtOAc) fraction from C. chinensis and in hexane (Hx) fraction from S. incisa, whereas inhibitory activities of W. subssilis were broadly seen in non-polar solvent fractions such as Hx, MC and EtOAc. Therefore, our results suggest that extracts from C. orbiculatus, C. chinensis, S. incisa and W. subsessilis may be developed as a therapeutic remedy against $TNF-{\alpha}-mediated$ diseases such as rheumatoid arthritis or further fractionated to isolate active components having $antiTNF-{\alpha}$ inhibitory activity.

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