Electricity Production from Fe[III]-reducing Bacterium Geobacter sulfurreducens in Microbial Fuel Cell

미생물 연료전지에서 Fe[III] 환원 미생물 Geobacter sulfurreducens를 이용한 전기 생산

  • Published : 2008.12.30

Abstract

Metal-reducing bacterium, Geobacter sulfurreducens is available for mediator-less microbial fuel cell (MFC) because it has biological nanowires(pili) which transfer electrons to outside the cell. In this study, in the anode chamber of the MFC system using G. sulfurreducens, the concentrations of NaCl, sodium phosphate and sodium bicarbonate as electrolytes were mainly optimized for the generation of electricity from acetate. 0.4%(w/v) NaClO and 0.5M $H_2SO_4$ could be utilized for the sterilization of acrylic plates and proton exchange membrane (major construction materials of the MFC reactor), respectively. When NaCl concentration in anode phosphate buffer increased from 5 to 50 mM, power density increased from 6 to $20\;mW/m^2$. However, with increasing sodium phosphate buffer concentration from 5 to 50 mM, power density significantly decreased from 18 to $1\;mW/m^2$. Twenty-four mM sodium bicarbonate did not affect electricity generation as well as pH under 50 mM phosphate buffer condition. Optimized anode chamber of MFC using G. sulfurreducens generated relatively high power density ($20\;mW/m^2$) with the maximum coulombic efficiency (41.3%).

Keywords

Geobacter sulfurreducens;Mediator-less microbial fuel cell;Electrolyte;pH control;Power density

References

  1. K. J. Chae, M. j. Choi, F. F, Ajayi, W. sh. Park, I. S. Chang and I. S. Kim, "Mass Transport through a Proton Exchange Membrane (Nafion) in Microbial Fuel Cell", Energy & Fuels, Vol. 22, 2008, pp. 169-176 https://doi.org/10.1021/ef700308u
  2. H. J. Kim, H. S. Park, M. S. Hyun, I. S. Chang, M. Kim and B. H. Kim, "A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefacien", Enzy. Microb. Technol., Vol. 30, 2002 , pp. 145-152 https://doi.org/10.1016/S0141-0229(01)00478-1
  3. Z. d. Liu and H. R. Li, "Effect of bio-and abio-factors on electricity production in a mediatorless microbial fuel cell", Biochem. Eng. J., Vol. 36, No. 3, 2007, pp. 209-214 https://doi.org/10.1016/j.bej.2007.02.021
  4. R. A. R., A. W. Jeremiasse, H. V.M. Hamelers and C. J. N. Buisman, "Hydrogen production with a Microbial Biocathode", Environ. Sci. Technol., Vol. 42, No. 2, 2008, pp. 629-634 https://doi.org/10.1021/es071720+
  5. G. Reguera, K. D. McCarthy, T. Mehta, J. S. Nicoll, M. T. Tuominen and D. R. Lovley, "Extracellular electron transfer via microbial nanowires", Nature, Vol. 435, No. 23, 2005, pp. 1098-1101 https://doi.org/10.1038/nature03661
  6. G. Reguera, K. P. Nevin, J. S. nicoll, S. F. Covalla, T. L. Woodard and D. R. Lovley, "Biofilm and Nanowire Production Leads To Increased Current in Geobacter Sulfurreducens Fuel Cells", Appl. Environ. Microbial., Vol. 72. No. 11, 2006, pp. 7345-7348 https://doi.org/10.1128/AEM.01444-06
  7. D. R. Lovley, "Bug juice; harvesting electricity with microorganisms", Nature reviews, Vol. 4, 2006, pp. 497-508 https://doi.org/10.1038/nrmicro1442
  8. D. R. Bond and D. R. Lovley, "Electricity Production by Geobacter sulfurreducens Attached to Electrode", Appl. Environ. Microbial., Vol. 69, No. 3, 2003, pp. 1548-1555 https://doi.org/10.1128/AEM.69.3.1548-1555.2003
  9. G. C. Gil, I. S. Chang, B. H. Kim, M. Kim, J. K. Jang, H. S. Park and H. J. Kim, "Operational parameters affecting the performance of a mediator-less microbial fuel cell", Biosens. and Bioelectronics, Vol. 18, 2003, pp. 327-334 https://doi.org/10.1016/S0956-5663(02)00110-0
  10. Pham, T. Hai, J. K. Jang, I. S. Jang and B. H. Kim "Improvrment of Cathode Reaction of a Mediatorless Microbial Fuel Cell", J. Microbial. Biotechnol., Vol. 14, No. 2, 2004, pp. 324-329
  11. D. call and B. E. Logan, "Hydrogen production in single chamber microbial electrolysis cell lacking a membrane", Environ. Sci. Technol., Vol. 42, No. 9, 2008, pp. 3401-3406 https://doi.org/10.1021/es8001822
  12. R. A. Rozendal, H. V.M. Hamelers and C. J. N. Buisman, "Effect of Membrane Cation Transfer on pH and Microbial Fuel Cell Performance", Environ. Sci. Technol, Vol. 40. No. 17, 2006, pp. 5206-5211 https://doi.org/10.1021/es060387r
  13. D. R. Bond, D. E. Holmes, L. M. Tender and D. R. Lovley, "Electrode-reducing Microorganism That Harvest Energy from Marine Sediments", Science, Vol. 295, 2002, pp. 483-485 https://doi.org/10.1126/science.1066771