Transactions of the Korean hydrogen and new energy society (한국수소및신에너지학회논문집)

The Korean Hydrogen and New Energy Society (한국수소및신에너지학회)
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Electricity Generation Using Cyanobacteria Synechocystis PCC 6803 in Photosynthetic Bio-Electrochemical Fuel Cell

남조류 Synechocystis PCC 6803을 이용한 생물전기화학적 물분해 전기 생산

  • Kim, Min-Jin (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Oh, You-Kwan (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Kim, Mi-Sun (Bioenergy Research Center, Korea Institute of Energy Research)
  • 김민진 (한국에너지기술연구원 바이오에너지연구센터) ;
  • 오유관 (한국에너지기술연구원 바이오에너지연구센터) ;
  • 김미선 (한국에너지기술연구원 바이오에너지연구센터)
  • Published : 2008.12.30

Abstract

Cyanobacteria Synechocystis PCC 6803 or the extracted thylakoid membrane from this strain was appled to photosynthetic bio-electrochemical fuel cell(PBEFC) for the production of hydrogen under the illumination of 48Klux using halogen lamp. PBEFC was composed of anode, cathode and membrane between them. Electrode material was carbon paper while electron mediator and receptor were added phenazine methosulfate(PMS) and potassium ferricyanide respectively. When water and 50 mM tricine buffer and $300{\mu}M$ PMS were added to the anode under the light condition, PBEFC produced the current density $4.4{\times}10^{-5}\;mA/cm^2$, $1.4{\times}10^{-4}\;mA/cm^2$ and $2.4{\times}10^{-4}\;mA/cm^2$, respectively. And the addition of the thylakoid membrane to the system increased current density to $1.3{\times}10^{-3}\;mA/cm^2$. Two times increase of the thylakoid membrane into the anode doubled the current density to $2.6{\times}10^{-3}\;mA/cm^2$. But the current density was not increased proportionally to the amount of thylakoid membrane increased. The system was unstable to measure the electricity output due to the foam production in the anode. Addition of triton X-100 and tween 80 stabilized the system to measure the electricity output but the current density was not increased higher than $8.4{\times}10^{-4}\;mA/cm^2$ and $2.3{\times}10^{-3}\;mA/cm^2$. When the thylakoid membrane was substituted to Synechocystis PCC 6803 cells of four-day culture which has chlorophyll contents $20.5{\mu}g/m{\ell}$, maximum current density was $1.3{\times}10^{-3}\;mA/cm^2$ with $1\;k{\Omega}$ resistance.

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References

  1. S. Tsujimura, A. Wadano, K. Kano, T. Ikeda, "Photosysnthetic BioelectroChemical Cell Utilizing Cyanobacteria and Water-Generating Oxidase", Enzyme and microbial Technology, Vol. 29, 2001, pp. 225-231 https://doi.org/10.1016/S0141-0229(01)00374-X
  2. T. Yagishita, Takashi, Horigome, K. Tanaka, "Effects of Light, $CO_2$ and Inhibitors on the Current output of Biofuel Cells Containing the Photosysnthetic Organism Synechocystis SP", J. Chem. Tech. Biotechnol, Vol. 56, 1993, pp. 393-399
  3. T. Yagishita, S. Sawayama, K. Tsukahara and T. OGI, "Photosysthetic Bio-Fuel Cells Using Cyanobacteria", Renewable Energy, Vol. 9, 1996, pp. 958-961 https://doi.org/10.1016/0960-1481(96)88439-4
  4. T. Yagishita, S. Sawayama, K. Tsukahara, and T. Ogi, "Effects of Glucose Addition and Light on Current Outputs in Photosynthetic Electrochemical Cells Using Synechocystis sp. PCC 6714", Journal of Bioscience and Bio-engineering. Vol. 88, No. 2, 1999, pp. 210-214 https://doi.org/10.1016/S1389-1723(99)80204-7
  5. K. Bang Lam, Eric A. Johnson, Mu Chiao, and Liwei Lin, "A MEMS Photosynthetic Electrochemical Cell Powered by Subcellular Plant Photosystems", Journal of microelectro- mechanical systems, Vol. 15, No. 5, 2006, pp. 1243-1250 https://doi.org/10.1109/JMEMS.2006.880296
  6. Mu Chiao, Kien B Lam and Liwei Lin, "Micromachined Microbial and Photosynthetic Fuel Cells", J. Micromech. Microeng, Vol. 16, 2006, pp. 2547-2553 https://doi.org/10.1088/0960-1317/16/12/005
  7. Elizabeth H. Harris, "The Chlamydomonas Sourcebook", Academic Press Inc., p. 608
  8. J. Komenda, L. Lupinkova, and J. Kopecky, "Absence of the psbF Gene Product Destabilizes Photosystem II Complex and Bicarbonate Binding on its Acceptor Side in Synechocystis PCC 6803", Eur. J. Biochem. Vol. 269, 2002, pp. 610-619 https://doi.org/10.1046/j.0014-2956.2001.02693.x
  9. K. Promnares, J. Komenda, L. Bumba, J. Nebesarova, Frantisek Vacha, and Martin Tichy, "Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits", Journal of Biological Chemistry, Vol. 281, No. 43, 2006, pp. 32705-32713 https://doi.org/10.1074/jbc.M606360200
  10. Z. Nemeth, G. Racz, and K. Koczo, "Foam Control by Silicone Polyethers-Mechanisms of 'Cloud Point Antifoaming'", Journal Of Colloid And Interface Science, Vol. 207, 1998, pp. 386-394 https://doi.org/10.1006/jcis.1998.5777
  11. S. Ritter, J. Komenda, E. Setlikova, I. Setlik and W. Welte, "Immobilized Metal Affinity Chromatography for the Separation of Photosystems I and II From the Thermophilic Cyanobacterium Synechococcus elongatus", Journal of Chromatography, Vol. 625, 1992, pp. 21-31 https://doi.org/10.1016/0021-9673(92)87217-V
  12. R. Bhardwaj, R. L. Pan and E. L. Gross, "Solar Energy Conversion by Chloroplast Photoelectrochemical Cells", Nature, Vol. 289, 1981, pp. 396-398 https://doi.org/10.1038/289396a0
  13. 최은혜, 오유관, 김미선, "홍색 유황세균 Thiocapsa reseopersicina가 생산하는 수소생산 효소의 정제 : Ammonium sulfate 침전 및 열처리의 효과", 한국수소 및 신에너지학회 논문집, Vol. 17, No. 2, 2006, pp. 371-378