Pre-ischemic Treatment with Ampicillin Reduces Neuronal Damage in the Mouse Hippocampus and Neostriatum after Transient Forebrain Ischemia

  • Lee, Kyung-Eon (Department of Pharmacology, Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Kim, Seul-Ki (Department of Pharmacology, Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Cho, Kyung-Ok (Department of Pharmacology, Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea) ;
  • Kim, Seong-Yun (Department of Pharmacology, Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea)
  • Published : 2008.12.31

Abstract

Ampicillin, a $\beta$-lactam antibiotic, has been reported to induce astrocytic glutamate transporter-l which plays a crucial role in protecting neurons against glutamate excitotoxicity. We investigated the effect of ampicillin on neuronal damage in the mouse hippocampus and neostriatum following transient global forebrain ischemia. Male C57BL/6 mice were anesthetized with halothane and subjected to bilateral occlusion of the common carotid artery for 40 min. Ampicillin was administered post-ischemically (for 3 days) and/or pre-ischemically (for $3{\sim}5$ days until one day before the onset of ischemia). Pre- and post-ischemic treatment with ampicillin (50 mg/kg/day or 200 mg/kg/day) prevented ischemic neuronal death in the medial CAI area of the hippocampus as well as the neostriatum in a dose-dependent manner. In addition, ischemic neuronal damage was reduced by pre-ischemic treatment with ampicillin (200 mg/kg/day). In summary, our results suggest that ampicillin plays a functional role as a chemical preconditioning agent that protects hippocampal neurons from ischemic insult.

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