Effect of Water Extract from Artemisiae Argi Folium on Hydrogen Peroxide Production within Mouse Macrophage Raw 264.7 Cells Treated with Gallic acid, EtOH, LPS, and Acetaminophen

Gallic acid, EtOH, LPS, Acetaminophen으로 유발된 마우스 대식세포 내 hydrogen peroxide 생성억제에 대한 애엽 물추출물의 영향 연구

  • Park, Wan-Su (Department of Pathology, College of Oriental Medicine, Kyungwon University)
  • 박완수 (경원대학교 한의과대학 병리학교실)
  • Published : 2008.12.25


The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium (WAAF) on hydrogen peroxide production within mouse macrophage Raw 264.7 Cells treated with gallic acid, EtOH, LPS, and acetaminophen. WAAF (0${\sim}$400 ug/mL) was treated with gallic acid, EtOH, LPS, acetaminophen. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. WAAF showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, LPS, and acetaminophen in Raw 264.7 Cells. WAAF could be supposed to have the immunological activity related with macrophage's oxidative burst.


  1. 전국한의과대학 공동교재편찬위원회. 본초학. 서울, 영림사, pp 405-407, 1991.
  2. 김호철. 한약약리학. 서울, 집문당, pp 309-311, 2001.
  3. Cao, H., Urban, J.F.Jr., Anderson, R.A. Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages. J Nutr. 138(5):833-840, 2008. https://doi.org/10.1093/jn/138.5.833
  4. Ripoll, V.M., Meadows, N.A., Raggatt, L.J., Chang, M.K., Pettit, A.R., Cassady, A.I., Hume, D.A. Microphthalmia transcription factor regulates the expression of the novel osteoclast factor GPNMB. Gene. 413(1-2):32-41, 2008. https://doi.org/10.1016/j.gene.2008.01.014
  5. Suzuki, Y., Funakoshi, H., Machide, M., Matsumoto, K., Nakamura, T. Regulation of cell migration and cytokine production by HGF-like protein (HLP) / macrophage stimulating protein (MSP) in primary microglia. Biomed Res. 29(2):77-84, 2008. https://doi.org/10.2220/biomedres.29.77
  6. Jirapongsananuruk, O., Malech, H.L., Kuhns, D.B., Niemela, J.E., Brown, M.R., Anderson-Cohen, M., Fleisher, T.A. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol. 111(2):374-379, 2003. https://doi.org/10.1067/mai.2003.58
  7. Richardson, M.P., Ayliffe, M.J., Helbert, M., Davies, E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods. 219(1-2):187-193, 1998. https://doi.org/10.1016/S0022-1759(98)00136-7
  8. Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide. 1(2):145-157, 1997. https://doi.org/10.1006/niox.1996.0113
  9. van Pelt, L.J., van Zwieten, R., Weening, R.S., Roos, D., Verhoeven, A.J., Bolscher, B.G. Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst. J Immunol Methods. 191(2):187-196, 1996. https://doi.org/10.1016/0022-1759(96)00024-5
  10. Roesler, J., Hecht, M., Freihorst, J., Lohmann-Matthes, M.L., Emmendörffer, A. Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry. Eur J Pediatr. 150(3):161-165, 1991. https://doi.org/10.1007/BF01963557
  11. Kim, M.J., Kim, D.H., Lee, K.W., Yoon, D.Y., Surh, Y.J. Jaceosidin induces apoptosis in ras-transformed human breast epithelial cells through generation of reactive oxygen species. Ann N Y Acad Sci. 1095: 483-495, 2007. https://doi.org/10.1196/annals.1397.052
  12. Jeong, M.A., Lee, K.W., Yoon, D.Y., Lee, H.J. Jaceosidin, a pharmacologically active flavone derived from Artemisia argyi, inhibits phorbol-ester-induced upregulation of COX-2 and MMP-9 by blocking phosphorylation of ERK-1 and -2 in cultured human mammary epithelial cells. Ann N Y Acad Sci. 1095: 458-466, 2007. https://doi.org/10.1196/annals.1397.049
  13. Lee, H.G., Yu, K.A., Oh, W.K., Baeg, T.W., Oh, H.C., Ahn, J.S., Jang, W.C., Kim, J.W., Lim, J.S., Choe, Y.K., Yoon, D.Y. Inhibitory effect of jaceosidin isolated from Artemisiaargyi on the function of E6 and E7 oncoproteins of HPV 16. J Ethnopharmacol. 98(3):339-343, 2005. https://doi.org/10.1016/j.jep.2005.01.054
  14. Adams, M., Efferth, T., Bauer, R. Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi. Planta Med. 72(9):862-864, 2006. https://doi.org/10.1055/s-2006-947165
  15. Lee, S.H., Kim, H.K., Seo, J.M., Kang, H.M., Kim, J.H., Son, K.H., Lee, H., Kwon, B.M., Shin, J., Seo, Y. Arteminolides B, C, and D, new inhibitors of farnesyl protein transferase from Artemisia argyi. J Org Chem. 67(22):7670-7675, 2002. https://doi.org/10.1021/jo020299z
  16. Tan, R., Jia, Z. Eudesmanolides and Other Constituents from Artemisia argyi. Planta Med. 58(4):370-372, 1992. https://doi.org/10.1055/s-2006-961488
  17. Gelderman, K.A., Hultqvist, M., Pizzolla, A., Zhao, M., Nandakumar, K.S., Mattsson, R., Holmdahl, R. Macrophages suppress T cell responses and arthritis development in mice by producing reactive oxygen species. J Clin Invest. 117(10):3020-3028, 2007. https://doi.org/10.1172/JCI31935
  18. Hultqvist, M., Bäcklund, J., Bauer, K., Gelderman, K.A., Holmdahl, R. Lack of reactive oxygen species breaks T cell tolerance to collagen type II and allows development of arthritis in mice. J Immunol. 179(3):1431-1437, 2007. https://doi.org/10.4049/jimmunol.179.3.1431
  19. Hultqvist, M., Olofsson, P., Holmberg, J., Bäckström, B.T., Tordsson, J., Holmdahl, R. Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene. Proc Natl Acad Sci U S A. 101(34):12646-12651, 2004.