Development of Multi-Residue Methods for Carbamate Pesticides by the Enzyme Inhibition Test

효소 저해법을 이용한 Carbamate계 농약의 다성분 잔류분석법 개발

  • Kim, Jung-Ho (Department of Safety & Environmental Prevention, Daegu Haanny University)
  • 김정호 (대구한의대학교 소방방재환경학과)
  • Published : 2008.12.31


This study was carried out with the detection for multiresidue of the carbamate pesticide such as carbaryl and cabofuran by enzyme-inhibition method. The check time for determination of acetylcholinesterase(AChE) activity was selected at 60 sec. The AChE activity in chicken brain determined by the Ellman's method was $162{\mu}$mol/min/g protein. $I_{50}$ for AChE by carbamate pesticide with wet kit was 0.169mg/L of carbaryl and 0.089mg/L of cabofuran, respectively. The incubation time for enzyme kit with substrate kit was 30min for determination of AChE activity. Enzyme kit with substrate kit was stable at $4^{\circ}C\;and\;25^{\circ}C$ for 5 days. Limit detection concentration of carbaryl with dry kit for AChE was 0.05mg/L. The dry kit such as wet kit applied Enzyme-Inhibition(EI) method with AChE was confirmed the multi residue method to detect the carbamate pesticides.


Enzyme inhibition;Acetylcholinesterase;Multiresidue method;Carbamate pesticides


  1. Bergmeyer H. H., 1984, Methods of enzymatic analysis, 3nd ed., Vol. IV, 52-74
  2. 유홍일, 이해근, 전성환, 1991, 농약잔류 분석방법, 동화기술
  3. Dzyadevych S. V., Chovelon J. M., 2002, A comparative photodegradation studies of methyl parathion by using lumistox test and conductometric biosensor technique. Materials and Engineering. C21, 55-60
  5. 김정호, 박흥재, 박병윤, 2002, Carbamate계 살충제에 의한 cholinesterase활성의 저해, 한국환경과학회지, 11(4), 391-397
  6. 김정호, 김영호, 1998, 유기인계 및 carbamates 농약에 의한 acetylcholinesterase 활성의 저해, 한국환경과학회지, 7(1), 52-56
  7. Eto M., 1974, Organophosphorus pesticides-organic and biological chemistry, CRC
  8. 김정호, 2002, 효소 저해법을 이용한 유기인계 및 carbamate계 농약의 다성분 잔류 검출, 환경독성학회지, 17(3), 265-272
  9. Kuhr R. J., 1977, Carbamate insecticides; chemistry, biochemistry, and toxicology, CRC, Ohio
  10. Collier W. A., Clear M., Hart A L., 2002, Convenient and rapid detection of pesticides in extracts of sheep wool. Biosensors and Bioelectronics, 17, 815-819
  11. 김정호, 2001, Cholinesterase 저해 활성을 이용한 유기인계 농약의 효소적 분석, 농약과학회지, 5, 12-18
  12. Ellman G. L., Courtney K. D., Andres Jr. V., Featherstone R. M., 1961, A new and rapid calorimetric determination of acetycholinesterase activity. Biochem. Pharmacol., 7, 88-95
  13. Tomlin C., 2000, The pesticide manual, 12th ed., British crop protection council, United Kingdom
  14. 김정호, 1987, 유기인계 살충제가 병아리의 acethylcholinesterase 활성에 미치는 영향, 박사학위논문, 경북대학교, 대구
  15. Matsumura F., 1975, Toxicology of insecticides, Plenum Press, New York, 105-164
  16. Lowry O. H., Roesbrough N. J., Favr A. L., Randall R. J., 1951, Protein measurement with the folin phenol reagent, J. Biol. Chem., 193, 265-275
  17. Albareda-Sirven M., Merkoci A., Alegret S., 2001, Pesticide determination in tap water and juice samples using disposable amperometric biosensors made using thick-film technology. Analytica Chimica Acta, 442, 35-44