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Development of Multi-Residue Methods for Carbamate Pesticides by the Enzyme Inhibition Test

효소 저해법을 이용한 Carbamate계 농약의 다성분 잔류분석법 개발

  • Kim, Jung-Ho (Department of Safety & Environmental Prevention, Daegu Haanny University)
  • 김정호 (대구한의대학교 소방방재환경학과)
  • Published : 2008.12.31

Abstract

This study was carried out with the detection for multiresidue of the carbamate pesticide such as carbaryl and cabofuran by enzyme-inhibition method. The check time for determination of acetylcholinesterase(AChE) activity was selected at 60 sec. The AChE activity in chicken brain determined by the Ellman's method was $162{\mu}$mol/min/g protein. $I_{50}$ for AChE by carbamate pesticide with wet kit was 0.169mg/L of carbaryl and 0.089mg/L of cabofuran, respectively. The incubation time for enzyme kit with substrate kit was 30min for determination of AChE activity. Enzyme kit with substrate kit was stable at $4^{\circ}C\;and\;25^{\circ}C$ for 5 days. Limit detection concentration of carbaryl with dry kit for AChE was 0.05mg/L. The dry kit such as wet kit applied Enzyme-Inhibition(EI) method with AChE was confirmed the multi residue method to detect the carbamate pesticides.

Keywords

Enzyme inhibition;Acetylcholinesterase;Multiresidue method;Carbamate pesticides

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