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ACE Inhibitory and Antioxidative Activities of Silkworm Larvae (Bombyx mori) Hydrolysate

번데기 가수분해물의 ACE 저해활성과 항산화활성

  • Yu, Jung-Sik (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Woo, Koan-Sik (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Youn-Ri (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kang, Tae-Su (Dept. of Biofood Science and Biotechnology, Chungbuk Provincial College of Science and Technology) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 유정식 (충북대학교 식품공학과) ;
  • 우관식 (충북대학교 식품공학과) ;
  • 황인국 (충북대학교 식품공학과) ;
  • 이연리 (충북대학교 식품공학과) ;
  • 강태수 (충북과학대학 바이오식품생명과학과) ;
  • 정헌상 (충북대학교 식품공학과)
  • Published : 2008.02.29

Abstract

In order to utilize the silkworm larvae (Bombyx mori) protein, defatted silkworm protein was hydrolysed by four enzymes (pepsin, trypsin, neutrase and alcalase) at various hydrolysis times (6, 12, 18, 24 and 30 hr) and suspension concentrations (2, 5, 10, 15 and 20%). Protein solubility index, ACE (angiotensin converting enzyme) inhibitory activity and antioxidative activity of silkworm protein hydrolysates were investigated. The optimum condition of hydrolysis was 10% suspension concentration and 18 hr. Protein solubility index of trypsin treatment was higher than other enzyme treatments. ACE inhibitory activity and $IC_{50}$ value of antioxidative activity of neutrase treatment were 86.16% at $100\;{\mu}g/mL$ and $352.75\;{\mu}g/mL$, respectively; also, these values were higher than other enzyme treatments.

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  1. Effect of Angiotensin-I Converting Enzyme Inhibitory from Hydrolysate of Soybean Protein Isolate vol.39, pp.1, 2010, https://doi.org/10.3746/jkfn.2010.39.1.008