Growth Inhibitory and Quinone Reductase Induction Activities of Salicornia herbacea L. Fractions on Human Cancer Cell Lines in vitro

함초 분획물의 in vitro에서의 암세포 성장억제 및 Quinone Reductase 활성 유도 효과

  • Jung, Bok-Mi (Dept. of Food Science and Nutrition, Yosu National University) ;
  • Park, Jung-Ae (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Bae, Song-Ja (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
  • 정복미 (여수대학교 식품영양학과) ;
  • 박정애 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터)
  • Published : 2008.02.29


We investigated the growth inhibitory effect of Salicornia herbacea L. (SH) on human cancer cell lines in vitro. SH was extracted with methanol (SHM), followed by further fractionation into four subfractions according to polarity: hexane (SHMH), methanol (SHMM), butanol (SHMB), and aqueous (SHMA) soluble fractions. We determined the growth inhibitory effect of these fractions against human cancer cell lines using MTT assay. Among the four subfractions of SHM, the SHMM showed the strongest cytotoxic effects on cancer cell lines. We also observed quinone reductase (QR)-inducing effect of methanol layer (SHMM) on HepG2 cells and it was determined to be 3.00 at $100\;{\mu}g/mL$ level compared to the control value of 1.0. The SHMM showed the highest induction activity of quinone reductase on HepG2 cells among the partition layers. The present work suggests that SH merits further study to confirm its chemopreventive potential.


  1. Hwang BH, Zhao JL, Choi KP, Jung SW, Kim EJ, Ham SS. 1996. The antimutagenic and anticancer effect of Taxus cuspidata extracts. J Korean Soc Food Sci Nutr 25: 1062-1068
  2. Yoo KY, Shin HR. 2003. Cancer epidemiology and prevention. Kor J Epidemiology 25: 1-15
  3. Bae MJ, Yee ST, Chae SY, Shin SH, Kweon SH, Park MH, Song MK, Hwang SJ. 2004. The effect of arabinoxylane and the polysaccharide peptide (PSP) on the antiallergy, anticancer. J Korean Soc Food Sci Nutr 33: 469-474
  4. Giampietri A. 1981. Drug-mediated increase of tumor immunogenicity in vivo for a new approach to experimental cancer immunotherapy. Cancer Res 41: 681-685
  5. Budd GT, Osgood B, Barna B, Boyett IM, Finke J, Mdendrop SV, Murth S, Bukoski RM. 1989. Phase I clinical trial toxicity and immunologic effects. Cancer Res 49: 6432-6439
  6. Kim JS. 2005. Cancer chemoprevention and NSAID-activated gene (NAG-1). Biochemistry and Molecular Biology News 25: 286-291
  7. Cha BC, Lee HW, Choi MY. 1998. Antioxidative and antimicrobial effects of nut species. Kor J Pharmacogn 29: 28-34
  8. Wefers H, Komai T, Talalay P, Sies H. 1984. Protection against reactive oxygen species by NAD(P)H:quinone reductase induced by the dietary antioxidant butylated hydroxyanisole (BHA). FEBS Lett 169: 63-66
  9. Schwartsmann G, Rocha AB, Berlinck RGS, Jimeno J. 2001. Marine organisms as a source of new anticancer agents. Lancet Oncology 2: 221-225
  10. Park JC, Choi JW. 1996. Screening of marine natural products on inhibitory effect of the formation of lipid peroxidation. Kor J Pharmacogn 27: 117-122
  11. Lee HJ, Kim YA, Ahn JW, Lee BJ, Moon SG, Seo YW. 2004. Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants. Kor J Biotechnol Bioeng 19: 57-61
  12. Rha ES. 2004. Utilization of halophyte for improving agricultural environment in China. Korean J Plant Res 17: 170-171
  13. Min JG, Lee DS, Kim TJ, Park DI. 2002. Chemical composition of Salicornia herbacea L. J Korean Soc Food Sci Nutr 7: 105-107
  14. Shimizu K. 2000. Effects of salt treatments on the production and chemical composition of salt wort (Salicornia herbacea L.), rhodesgrass and alfalfa. Jpn J Trop Agr 44: 61-67
  15. Park SH, Kim KS. 2004. Isolation and identification of antioxidant flavonoids from Salicornia herbacea. J Korean Soc Appl Biol Chem 47: 120-123
  16. Shin KS, Boo HO, Jeon MW, Ko JY. 2002. Chemical components of native plant, Salicornia herbacea L. Korean J Plant Res 15: 216-220
  17. Han SK, Kim SM. 2003. Antioxidative effect of Salicornia herbacea L. grown in closed sea beach. J Korean Soc Food Sci Nutr 21: 207-210
  18. Son DY, Ermawati N, Cha JY, Liang YS, Jung MH, Shin DJ, Lee BH, Lee KH. 2004. Molecular cloning and characterization of outer envelope membrane protein from Salicornia herbacea. J Plant Biotechnol 31: 273-278
  19. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, Abbortt BJ, Mayo JG, Shoemarker RH, Boyd MR. 1988. Feasibility of drug screening with panels of human tumor cell line using a microculture tetrazolium assay. Cancer Res 48: 589-601
  20. Carmichael J, De Graff WG, Gazder AF, Minna JD, Mitchell JB. 1987. Evaluation of the tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942
  21. Hong EY, Kang HJ, Kwan CS, Na YJ, Suh MJ, Kim JS. 1997. Modulation of cellular quinone reductase inducibility by roasting treatment and acid hydrolysis of perilla. J Korean Soc Food Sci Nutr 26: 186-192
  22. Prochaska HJ, Santamaria AB. 1988. Direct measurement of NAD(P)H:quinone reductase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme inducers. Anal Biochem 169: 328-336
  23. Prochaska HJ, Santamaria AB, Talalay P. 1992. Rapid detection of inducers of enzyme that protect against carcinogen. Proc Natl Acad Sci USA 89: 2394-2398
  24. Prochaska HJ. 1994. Screening strategies for the detection of anticarcinogenic enzyme inducers. J Nutr Biochem 5: 360-366
  25. Park SY, Joung YH, Shin MO, Joung BM, Bae SJ. 2005. Effect of antimicrobial and cytotoxicity of Undaria pinnatifida Sporophyll fractions. J Korean Soc Food Sci Nutr 34: 765-770

Cited by

  1. Physiological Activities of Suaeda japonica Extracts on Harvest Season vol.39, pp.1, 2010,
  2. Two new noroleanane-type triterpene saponins from the methanol extract of Salicornia herbacea vol.151, 2014,
  3. Anti-obesity effect of Korean Hamcho (Salicornia herbaceaL.) powder on high-fat diet-induced obese rats vol.48, pp.2, 2015,
  4. Physicochemical Characteristics and Physiological Activities of Naturally Fermented Glasswort (Salicornia herbacea L.) Juice vol.40, pp.11, 2011,
  5. Antioxidant Activity and Quality Characteristics of Pork Patties Added with Saltwort (Salicornia herbacea L.) Powder vol.43, pp.8, 2014,
  6. Quality Characteristic of Glasswort (Salicornia herbacea L.) Fermented by Bacillus subtilis vol.38, pp.7, 2009,
  7. Antioxidant properties and cytotoxic effects of fractions from glasswort (Salicornia herbacea) seed extracts on human intestinal cells vol.20, pp.1, 2011,
  8. Changes in Physicochemical Properties of Spergularia marina Griseb by Blanching vol.19, pp.6, 2012,
  9. Hypolipidemic and antioxidant effects on hypercholesterolemic rats of polysaccharide from Salicornia bigelovii seed vol.34, pp.3, 2017,
  10. Acute Oral Toxicity ofSalicornia herbaceaL. Extract in Mice vol.22, pp.2, 2016,
  11. Modulation of the inflammatory process and interaction of THP-1 monocytes with intestinal epithelial cells by glasswort (Salicornia herbacea L.) extracts vol.48, pp.4, 2016,
  12. Antiadipogenic Effect of Korean Glasswort (Salicornia herbacea L.) Water Extract on 3T3-L1 Adipocytes vol.43, pp.6, 2014,
  13. Four New Dicaffeoylquinic Acid Derivatives from Glasswort (Salicornia herbacea L.) and Their Antioxidative Activity vol.21, pp.8, 2016,
  14. Characterization, stability, and antioxidant activity of Salicornia herbaciea seed oil vol.31, pp.12, 2014,
  15. Changes in Antioxidant and Cancer Cell Growth Inhibitory Activities of Spergularia marina Griseb Extract according to Different Cooking Methods vol.33, pp.6, 2017,