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Lipase-Inhibitory and Anti-Oxidative Activity of the Methanol Extract and the Powder of Phellinus linteus

상황버섯 자실체 메탄올 추출물과 분말의 지방소화효소 억제 및 항산화 활성

  • Kim, Ji-Hyun (Dept. of Food Science and Nutrition, Andong National University) ;
  • Son, In-Suk (Dept. of Food Science and Nutrition, Andong National University) ;
  • Kim, Jong-Sang (Dept. of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Ki-Hoon (Andongsi Agricultural Technology Center) ;
  • Kwon, Chong-Suk (Dept. of Food Science and Nutrition, Andong National University)
  • 김지현 (안동대학교 생활과학대학 식품영양학과) ;
  • 손인숙 (안동대학교 생활과학대학 식품영양학과) ;
  • 김정상 (경북대학교 동물공학과) ;
  • 김기훈 (안동시 농업기술센터) ;
  • 권정숙 (안동대학교 생활과학대학 식품영양학과)
  • Published : 2008.02.29

Abstract

Phellinus linteus (PL) has been known to exhibit potent biological activity. The present study was designed to investigate lipase-inhibitory and anti-oxidative activity of the methanol extract and the powder of PL fruiting body. The methanol extract of PL appeared to have the inhibitory activity against pancreatic lipase with an $IC_{50}$ value of $36.3\;{\mu}g/mL$, and the scavenging activity of DPPH radical with an $IC_{50}$ value of $20.1\;{\mu}g/mL$, which was similar to that of vitamin C ($IC_{50}\;18.3\;{\mu}g/mL$). To investigate the lipase-inhibitory and anti-oxidative effect of PL on animal, Sprague-Dawley rats were fed with high-fat diet supplemented with either 2% or 5% PL powder for 8 weeks. Total food intake was significantly increased, but body weight was not changed by PL powder supplementation. However, fecal fat excretion of the experimental groups fed with the PL powder were higher than that of the control group. PL powder showed a decrease in the plasma total cholesterol, LDL-cholesterol, and the hepatic total cholesterol levels. The anti-oxidative enzyme activities were also affected by PL supplementation. Glutathione peroxidase (GSH-Px) in the plasma and liver were significantly increased by 98% and 46% in the 2% PL group, and 99% and 32% in the 5% PL group, respectively. Total superoxide dismutase (T-SOD) activity was not affected by PL supplementation. DNA damage was measured by the comet assay in the lymphocytes collected after 2 weeks, 4 weeks, and 8 weeks of feeding PL supplemented diet. Lymphocyte DNA damage was decreased in the PL supplemented group. Furthermore, PL feeding enhanced the resistance to lymphocyte DNA damage caused by an oxidant challenge with $H_2O_2$.

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