Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Lipase-Inhibitory and Anti-Oxidative Activity of the Methanol Extract and the Powder of Phellinus linteus

상황버섯 자실체 메탄올 추출물과 분말의 지방소화효소 억제 및 항산화 활성

  • Kim, Ji-Hyun (Dept. of Food Science and Nutrition, Andong National University) ;
  • Son, In-Suk (Dept. of Food Science and Nutrition, Andong National University) ;
  • Kim, Jong-Sang (Dept. of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Ki-Hoon (Andongsi Agricultural Technology Center) ;
  • Kwon, Chong-Suk (Dept. of Food Science and Nutrition, Andong National University)
  • 김지현 (안동대학교 생활과학대학 식품영양학과) ;
  • 손인숙 (안동대학교 생활과학대학 식품영양학과) ;
  • 김정상 (경북대학교 동물공학과) ;
  • 김기훈 (안동시 농업기술센터) ;
  • 권정숙 (안동대학교 생활과학대학 식품영양학과)
  • Published : 2008.02.29

Abstract

Phellinus linteus (PL) has been known to exhibit potent biological activity. The present study was designed to investigate lipase-inhibitory and anti-oxidative activity of the methanol extract and the powder of PL fruiting body. The methanol extract of PL appeared to have the inhibitory activity against pancreatic lipase with an $IC_{50}$ value of $36.3\;{\mu}g/mL$, and the scavenging activity of DPPH radical with an $IC_{50}$ value of $20.1\;{\mu}g/mL$, which was similar to that of vitamin C ($IC_{50}\;18.3\;{\mu}g/mL$). To investigate the lipase-inhibitory and anti-oxidative effect of PL on animal, Sprague-Dawley rats were fed with high-fat diet supplemented with either 2% or 5% PL powder for 8 weeks. Total food intake was significantly increased, but body weight was not changed by PL powder supplementation. However, fecal fat excretion of the experimental groups fed with the PL powder were higher than that of the control group. PL powder showed a decrease in the plasma total cholesterol, LDL-cholesterol, and the hepatic total cholesterol levels. The anti-oxidative enzyme activities were also affected by PL supplementation. Glutathione peroxidase (GSH-Px) in the plasma and liver were significantly increased by 98% and 46% in the 2% PL group, and 99% and 32% in the 5% PL group, respectively. Total superoxide dismutase (T-SOD) activity was not affected by PL supplementation. DNA damage was measured by the comet assay in the lymphocytes collected after 2 weeks, 4 weeks, and 8 weeks of feeding PL supplemented diet. Lymphocyte DNA damage was decreased in the PL supplemented group. Furthermore, PL feeding enhanced the resistance to lymphocyte DNA damage caused by an oxidant challenge with $H_2O_2$.

Keywords

File

Download PDF

References

  1. Ministry of Health and Welfare. 2006. The Third Korea National Health and Nutrition Examination Survey (KNHANES III), Nutrition Survey (1), 2005. p 229
  2. Ministry of Health and Welfare. 2006. The Third Korea National Health and Nutrition Examination Survey (KNHANES III), Health Examination, 2005. p 38-44
  3. Bray GA, Ryan DH. 2007. Drug treatment of the overweight patient. Gastroenterology 132: 2239-2252 https://doi.org/10.1053/j.gastro.2007.03.053
  4. Vincent HK, Innes KE, Vincent KR. 2007. Oxidative stress and potential interventions to reduce oxidative stress in overweight and obesity. Diabetes Obes Metab 9: 813-839 https://doi.org/10.1111/j.1463-1326.2007.00692.x
  5. Carantoni M, Abbasi F, Warmerdam F, Klebanov M, Wang PW, Chen YD, Azhar S, Reaven GM. 1998. Relationship between insulin resistance and partially oxidized LDL particles in healthy, nondiabetic volunteers. Arterioscler Thromb Vasc Biol 18: 762-767 https://doi.org/10.1161/01.ATV.18.5.762
  6. Hirai N, Kawano H, Hirashima O, Motoyama T, Moriyama Y, Sakamoto T, Kugiyama K, Ogawa H, Nakao K, Yasue H. 2000. Insulin resistance and endothelial dysfunction in smokers: effects of vitamin C. Am J Physiol Heart Circ Physiol 279: H1172-1178
  7. Ikegawa T, Nakanishi M, Uehara N, Chihara G, Fukuoka F. 1968. Antitumor action of some Basidiomycetes, especially Phellinus linteus. Gann 59: 155-157
  8. Song KS, Cho SM, Lee JH, Kim HM, Han SB, Ko KS, Yoo ID. 1995. B-lymphocyte stimulating polysaccharide from mushroom Phellinus linteus. Chem Pharm Bull 43: 2105- 2108 https://doi.org/10.1248/cpb.43.2105
  9. Lee JH, Cho SM, Song KS, Han SB, Kim HM, Hong ND, Yoo ID. 1996. Immunostimulating activity and characterization of polysaccharide from mycelium of Phellinus linteus. J Microbiol Biotechnol 6: 213-218
  10. Han SB, Lee CW, Jeon YJ, Hong ND, Yoo ID, Yang KH, Kim HM. 1999. The inhibitory effect of polysaccharides isolated from Phellinus linteus on tumor growth and metastasis. Immunopharmacology 41: 157-164 https://doi.org/10.1016/S0162-3109(98)00063-0
  11. Song YS, Kim SH, Sa JH, Jin C, Lim CJ, Park EH. 2003. Anti-angiogenic, antioxidant and xanthine oxidase inhibition activities of the mushroom Phellinus linteus. J Ethnopharmcol 88: 113-116 https://doi.org/10.1016/S0378-8741(03)00178-8
  12. Kwoen DJ, Youn SJ, Cho JG, Choi UK, Kang SC. 2006. Antioxidant activities and biological properties of Phellinus linteus extracts according to different extraction methods. J Korean Aoc Appl Biol Chem 49: 91-96
  13. Shon MY, Seo KI, Chio SY, Sung NJ, Lee SW, Park SK. 2006. Chemical compounds and biological activity of Phellinus baumii. J Korean Soc Food Sci Nutr 35: 524-529 https://doi.org/10.3746/jkfn.2006.35.5.524
  14. Atsumi S, Nosaka C, Ochi Y, Iinuma H, Umezawa K. 1993. Inhibition of experimental metastasis by an $\alpha$-glucosidase inhibitor, 1,6-epi-cyclophellitol. Cancer Res 53: 4896-4899
  15. AOAC. 2000. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington DC
  16. Prosky L, Asp NG, Furda I, DeVries JW, Schweizer TF, Harland BF. 1985. Determination of total dietary fiber in foods and food products: collaborative study. J Assoc Off Anal Chem 68: 677-679
  17. Kawaguchi K, Mizuno T, Aida K, Uchino K. 1997. Hesperidin as an inhibitor of lipases from porcine pancreas and Pseudomonas. Biosci Biotechnol Biochem 61: 102-104 https://doi.org/10.1271/bbb.61.102
  18. Bitou N, Ninomiya M, Tsujita T, Okuda H. 1999. Screening of lipase inhibitors from marine algae. Lipids 34: 441-445 https://doi.org/10.1007/s11745-999-0383-7
  19. Hatano T, Kagawa H, Yasuhara T, Okuda T. 1988. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem Pharm Bull 36: 2090-2097 https://doi.org/10.1248/cpb.36.2090
  20. Friedewald WT, Levy RI, Fredrickson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502
  21. Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158-169
  22. Flohe L, Otting F. 1984. Superoxide dismutase assays. Methods Enzymol 105: 93-104 https://doi.org/10.1016/S0076-6879(84)05013-8
  23. Singh NP, McCoy MT, Tice RR, Schneider EL. 1998. A simple technique for quantitaion of low levels of DNA damage in individual cells. Exp Cell Res 175: 184-191 https://doi.org/10.1016/0014-4827(88)90265-0
  24. Hwang EI, Kim JR, Jeong TS, Lee S, Rho MC, Kim SU. 2006. Phellinsin A from Phellinus sp. PL3 exhibits antioxidant activities. Planta Med 72: 572-575 https://doi.org/10.1055/s-2006-931533
  25. Jung ME, Ham SS, Nam SM, Kang IJ, Kim SJ, Chung CK. 2001. Biochemical and histological effects of Phellinus linteus methanol extract on liver lipid metabolism of rats fed CCl4 and high fat. J Korean Soc Food Sci Nutr 30: 331-337
  26. Kim HM, Han SB, Oh GT, Kim YH, Hong DH, Hong ND, Yoo ID. 1996. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int J Immunopharmacol 18: 295-303 https://doi.org/10.1016/0192-0561(96)00028-8
  27. Ye SF, Hou ZQ, Zhang QQ. 2007. Protective effects of Phellinus linteus extract against iron overload-mediated oxidative stress in cultured rat hepatocytes. Phytother Res 21: 948-953 https://doi.org/10.1002/ptr.2182
  28. Guemouri L, Artur Y, Herbeth B. 1991. Biological variability of superoxide dismutase, glutathione peroxidase, and catalase in blood. Clin Chem 37: 1932-1937
  29. Fridovich I. 1978. The biology of oxygen radicals. Science 201: 875-880 https://doi.org/10.1126/science.210504
  30. Emerit J, Beaumount C, Trivin F. 2001. Iron metabolism, free radicals, and oxidative injury. Biomed Pharmacother 55: 333-339 https://doi.org/10.1016/S0753-3322(01)00068-3
  31. Shon YH, Nam KS. 2001. Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes. J Ethnopharmacol 77: 103-109 https://doi.org/10.1016/S0378-8741(01)00276-8
  32. Park J, Lee BR, Jin LH, Kim CK, Choi KS, Barn JH, Lee KS, Kwon HY, Chang HW, Baek NI, Lee EH, Kang JH, Cho SW, Choi SY. 2001. The stimulatory effect of Ganoderma lucidum and Phellinus linteus on the antioxidant enzyme catalase. J Biochem Molecul Biol 34: 144-149
  33. Ji JH, Kim MN, Chung CK, Ham SS. 2000. Antimutagenic and cytotoxicity effects of Phellinus linteus extracts. J Korean Soc Food Sci Nutr 29: 322-328
  34. Ji JH, Kim MN, Chung CK, Ham SS. 2000. Antigenotoxic effects of Phellinus linteus and Agaricus blazei Murill extracts. J Korean Soc Food Sci Nutr 29: 513-517

Cited by

  1. Antioxidant and Anti-inflammatory Activities of Functional Plant Materials vol.23, pp.7, 2013, https://doi.org/10.5352/JLS.2013.23.7.869
  2. Antioxidant capacities and β-glucan content of ethanol extract from Phellinus baumii vol.22, pp.5, 2015, https://doi.org/10.11002/kjfp.2015.22.5.721
  3. Inhibitory Effects of Phellinus linteus and Rice with Phellinus linteus Mycelium on Obesity and Diabetes vol.42, pp.7, 2013, https://doi.org/10.3746/jkfn.2013.42.7.1029
  4. Quality Characteristics of Yanggaeng Supplemented with Sanghwang Mushroom (Phellinus linteus) Mycelia vol.19, pp.3, 2013, https://doi.org/10.14373/JKDA.2013.19.3.253
  5. Physiological Activities of Korean Traditional Soybean-Fermented Royal Court Soy Sauces, Gungjungjang vol.41, pp.2, 2012, https://doi.org/10.3746/jkfn.2012.41.2.149
  6. Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus : A review vol.113, 2016, https://doi.org/10.1016/j.fitote.2016.06.009
  7. Anti-oxidative and Anti-hyperglycemia Effects of Dung Beetle Extracts on the High Fat Diet SD Rats vol.26, pp.7, 2016, https://doi.org/10.5352/JLS.2016.26.7.772