Effects of Samgi-eum(Sānqì-yǐn) Treatment on the Monosodium Iodoacetate-induced Osteoarthritis in Rats

삼기음(三氣飮)이 흰쥐의 Monosodium Iodoacetate 유발 골관절염에 미치는 영향

  • Lee, Kyung-Moo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Jeong, Su-Hyeon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Soon-Joong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bok (Dept. of Anatomy, College of Oriental Medicine, Se-Myung University)
  • 이경무 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 정수현 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 김순중 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실)
  • Received : 2008.03.17
  • Accepted : 2008.04.16
  • Published : 2008.04.30
  • 23 0


Abstract

Objectives : This study was carried out to investigate the effects of Samgi-eum($S{\bar{a}}nq{\grave{i}}-y{\check{i}}n$) on the monosodium iodoacetate-induced osteoarthritis in rats. Methods : Osteoarthritis was induced by injection of monosodium iodoacetate intraarticularly in both knee joints. Arthritic rats were divided into control and treated group. Control group were taken distilled water for 20days. Treated group were taken extracts of Samgi-eum($S{\bar{a}}nq{\grave{i}}-y{\check{i}}n$) by orally for the same duration. Normal group were injected normal saline and taken distilled water. Body weights were measured at 0, 5th, 10th, 15th, 20th day after injection. At the end of the experiment, gross and histopathological examination on the articular cartilages of the knee joints were performed. Proteoglycan contents of articular cartilages were analyzed by safranine O staining method. The contents of $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6 in synovial fluids were analyzed by ELISA method. Results : 1. Body weights of the treated group were significantly increased compared with control at 20days after injection. 2. Grossly, the severity of osteoarthritis in the treated group were alleviated compared with control. 3. Histopathologically, degenerative and necrotic lesion of articular cartilages in the treated group were alleviated compared with those of the control and histopathological scores of treated group were significantly decreased compared with control. 4. PG contents in articular cartilages of the treated group were significantly increased compared with control. 5. $TNF-{\alpha}$ contents in synovial fluids of the treated group were significantly decreased compared with control. Conclusions : According to above results, Samgi-eum($S{\bar{a}}nq{\grave{i}}-y{\check{i}}n$) has anti-arthritic effects on the monosodium iodoacetate-induced osteoarthritis in rats. And it is related with reduced secretion of $TNF-{\alpha}$ from osteoarthritic chondrocytes and synovial membranes.

Keywords

Samgi-eum($S{\bar{a}}nq{\grave{i}}-y{\check{i}}n$);Osteoarthritis;Proteoglycan;$TNF-{\alpha}$