The Inhibitory Effects of Gamipalmul-tang(jiaweibawu-tāng) on the Obese-Mice Induced by High Fat Diet

가미팔물탕(加味八物湯)이 생쥐의 비만억제에 미치는 영향

  • Kim, Hyo-Young (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Heo, Dong-Seok (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University)
  • 김효영 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 허동석 (대전대학교 한의과대학 한방재활의학과교실)
  • Received : 2008.03.11
  • Accepted : 2008.04.10
  • Published : 2008.04.30
  • 17 0


Abstract

Objectives : This study was performed to investigate inhibitory effects of Gamipalmul-tang($jiaweibawu-t{\bar{a}}ng$) on the hematological and histological changes of obese mice. Methods : C57BL/6 mice were divided into four groups (normal group, high fat diet with normal saline, high fat diet with reductil, high fat diet with Gamipalmul-tang($jiaweibawu-t{\bar{a}}ng$) and fed for 8 weeks. Body weight change, final increase of body weight, ALT, AST, creatinine, total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, glucose, cell viability by cytotoxicity, the expression of ${\beta}3AR$ in 3T3-L1 cell, the expression of leptin, ${\beta}3AR$ and serotonin in adipocyte tissue and size of adipocyte were observed in 8 weeks. Results : 1. In 3T3-L1 cell. the expression of ${\beta}3AR$ was increased significantly. 2. The final increase of body weight in obese-mouse were decreased significantly. 3. The level of AST, ALT were decreased significantly. 4. The level of LDL-cholesterol was decreased significantly and HDL-cholesterol was increased significantly. 5. The levels of triglyceride was decreased and leptin and glucose were decreased significantly. 6. In adipocyte tissue, the expression of ${\beta}3AR$ were increased significantly. 7. In adipocyte tissue, the expression of leptin and serotonin were decreased significantly. 8. The size of Adipocyte was decreased. Conclusions : On the basis of these results, we concluded that Gamipalmul-tang($jiaweibawu-t{\bar{a}}ng$) has inhibitory effects in rat.

Keywords

Gamipalmul-tang($jiaweibawu-t{\bar{a}}ng$);Obesity;Body wight;Aiypocyte