DOI QR코드

DOI QR Code

The Effects of Houttuyniae Herba extract on the Activity of Anti-bacteria, Anti-inflammation and Anti-oxidation

어성초(魚腥草) 추출물의 항여드름 효과에 관한 연구

  • Jeon, Oh-do (Dept. of Ophthalmology, Otorhinolaryngology & Dermatology, College of Korean Medicine, Sangji University) ;
  • Seo, Hyeong-Sik (Dept. of Ophthalmology, Otorhinolaryngology & Dermatology, College of Korean Medicine, Sangji University)
  • 전오도 (상지대학교 한의과대학 안이비인후피부과 교실) ;
  • 서형식 (상지대학교 한의과대학 안이비인후피부과 교실)
  • Published : 2008.03.31

Abstract

Objective : This experimental study was performed to investigate the effects of Houttuyniae Herba extract on anti-inflammation and anti-oxidation. Methods : The cytotoxicity of Houttuyniae Herba water extract and ethanol extract about viability of Raw 264.7 cell were tested using a colormetric tetrazolium assay(MTT assay). We investigated the inhibitory effects of Houttuyniae Herba water extract and ethanol extract on Propionibactrium acnes using paper disk diffusion method. To investigate the anti-inflammation effects of Houttuyniae Herba water extract and ethanol extract on LPS-induced macrophage Raw 264.7 cell, we used ELISA kit. We evaluated anti-oxidation effects of Houttuyniae Herba water extract and ethanol extract on HaCaT cell by Enzyme recycling method. Results : 1. In Houttuyniae Herba water extract and ethanol extract, cell toxicity depended on the density and wasn't difference between two extracts. 2. Houttuyniae Herba water extract and ethanol extract has not the significant inhibition effect of Propionibactrium acnes. 3. Concentration of 50, $100{\mu}g/m{\ell}$ Houttuyniae Herba water extract inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 4. All extracts except for $20{\mu}g/m{\ell}$ Houttuyniae Herba water extract showed anti-oxidation effect by decreasing the DPPH radicals. Conclusion : These results indicate that Houttuyniae Herba extract has anti-inflammation and anti-oxidation effects. If further study is performed, the use of Houttuyniae Herba extract will be valuable and benificial in the therapy of Propionibactrium acnes.

References

  1. 안성구 외. 여드름 바이블. 서울. 진솔. 2006:62
  2. 강경희 외. 피부과학. 서울. 청구문화사. 2002:127-130
  3. 황규왕. 여드름의 치료. 대한의사협회지. 2006;49(1):63-64
  4. 의과대학 교수편. 임상약리학. 서울. 한우리. 1998:1138-1139
  5. 서부일 외. 알기쉬운 본초학. 경산. 대구한의대학교 출판부.2004:97-98
  6. 안덕균. 한국본초도감. 서울. 교학사. 2000:9
  7. H. Kim, H. Lim, B. Kim, H. Kim, S. Choi, C. Yoon, Studies on the anti-acne effect of Agrimonia pilosa Ledeb, J. Soc. Cosmet. Scientists Korea, 2006;32(1):53-8
  8. Trichophyton and Pityrosporum., S. Lee, Antimicrobial effect of Bamboo (Phyllosrachys Bambusoides) Essential Oil on, J. Fd Hyg. Safety, 2003;18(3):113-7
  9. T. Mosmann, Rapid colorimetric assay for cellular growth and survival application of proliferation and cytotoxicity, J. Immunol. Methods, 1983;65:55 https://doi.org/10.1016/0022-1759(83)90303-4
  10. S. M. Han, K. G. Lee, J. H. Yeao, H. Y. Kweon, and K. K. Park, Anti-inflammatory effect of the venom from asian honeybees(Apis cerana L.) on inducible nitric oxide synthase and tumor necrosis factor-${\alpha}$ in Raw 264.7 cell line, Korean J. Apiculture, 2004;19:89
  11. M. S. Blois, Antioxidant determinanations by the use of a stable free radical, Nature, 1958;181:1199 https://doi.org/10.1038/1811199a0
  12. 陳實功. 外科正宗. 천진. 천진과학기술출판사. 2000:281
  13. 伯외. 실용중의외과학. 상해. 상해과학기술출판사. 1985:535-6
  14. 안성구 외. 여드름 바이블. 서울. 진솔. 2006:263, 347
  15. 신민교. 임상본초학. 서울.영림사. 2000:574-578
  16. 안은경. 천마 에탄올 추출물에 대한 항염증 활성. 숙명여자대학교 약학과 석사논문.2006:3
  17. 하대식. 전통 약용식물의 항산화효과와 유전 독성에 관한 연구. 경상대 대학원 수의학과 박사논문.2006:37-38
  18. 두인선. 5% 어성초 추출물 수용액의 외용요법이 여드름에 미치는 임상적 연구. 원광대 대학원 석사논문. 2004:39-40
  19. 전국한의과대학 본초학교실. 본초학. 서울. 영림사. 1991:212-213
  20. Nam-Kyoung Kim, Jae-Hyung Jung, Shin wook choi, Chang-soon Yoon, Studies on the antioxidative effect of the BuckWheat (Fagopyrum esculentum Moench) extract and its Protective Role against Cadmium-mediated stress, J. Soc. Cosmet. Scientists Korea, 2005;31(2):197-206
  21. 李經緯외. 중의대사전. 북경. 인민위생출판사. 1995:1285
  22. 오성문. 약용식물로부터 여드름 원인 미생물에 대한 천연 항균제 탐색. 서울:연세대 공학대학원 석사논문. 2006:12-17
  23. 서영국. 어성초 즙 발효제품의 항산화 및 항알레르기 활성. 순천대 대학원. 식품영양학과 석사논문. 2005:15
  24. Sang Eun Lee, Dong Wook Son, Year Pill Yoon, Tae Young Lim, and Haen Su Kim, J. Screening of Traditional Herbral Medicines to Develop New Materials for Anti-aging and Anti-wrinkle in the skin, Soc. Cosmet. Scientists Korea, 2005; 31(2):147-52

Cited by

  1. Review on the Acne Related Articles Published in Korean Medical Journals - Focusing on Experimental Studies Published after 2005 - vol.28, pp.1, 2015, https://doi.org/10.15204/jkobgy.2015.28.1.113