Effects of Monensin and Fish Oil on Conjugated Linoleic Acid Production by Rumen Microbes in Holstein Cows Fed Diets Supplemented with Soybean Oil and Sodium Bicarbonate

DOI QR코드

DOI QR Code

Jin, G.L.;Choi, S.H.;Lee, H.G.;Kim, Y.J.;Song, Man K.

  • 투고 : 2008.02.12
  • 심사 : 2008.06.05
  • 발행 : 2008.12.01

초록

The present study was conducted with four ruminally canulated Holstein cows to observe the effects of monensin or fish oil on diet fermentation and production of conjugated linoleic acids (CLAs) in the rumen when fed diets supplemented with soybean oil and sodium bicarbonate. Cows of the control treatment were fed a basal diet (CON) consisting of 60% commercial concentrate and 40% chopped rye grass hay. Cows of other treatments were fed the same diet as CON, but the concentrate was supplemented with 7% of soybean oil and 0.5% of sodium bicarbonate (SO-B), SO-B supplemented with monensin (30 ppm, SO-BM) or concentrate supplemented with 6.3% of soybean oil, 0.5% of sodium-bicarbonate, 30 ppm of monensin and 0.7% of fish oil (SO-BMF). Dry matter (DM) intake of the cows was significantly (p<0.011) reduced by feeding the SO-BMF diet compared to the other diets which did not differ in DM intake. Whole tract digestibility of major dietary components was significantly (p<0.004-0.027) higher for SO-BMF than the other supplement-containing diets. Dietary supplements did not clearly affect rumen pH and ammonia concentrations compared to the CON diet. Significantly reduced (p<0.05) total VFA concentration was obtained by the addition of fish oil to the diet (SO-BMF) compared to other diets. No differences, however, were obtained in major VFA proportions as well as in total VFA between the supplemented diets. The SO-BM diet increased (p<0.01-0.05) the concentrations of trans-11 $C_{18:1}$ and linoleic acid in rumen fluid. Total CLA concentration was also increased by the feeding of SO-B and SO-BM diets during early fermentation times (up to 3 h) post-feeding. Cis-9, trans-11 CLA concentration in rumen fluid was highest (p<0.05) for SO-B up to 1 h while the highest (p<0.01) value for SO-BM occurred at 3 h post-feeding. An increased trans-10, cis-12 CLA concentration was obtained from the SO-B and SO-BM diets at 1 and 3 h post feeding compared to the other diets. Supplementation of oils with monensin and sodium bicarbonate increased (p<0.05) the proportions of $C_{18:1}$ and CLA in the plasma of cows, but the effect of monensin and/or fish oil was limited to trans-10, cis-12 CLA.

키워드

Monensin;Fish Oil;Soybean Oil;Buffer;CLA;Fatty Acid Composition;Biohydrogenation;Rumen Microbes;Plasma;Holstein Cows

참고문헌

  1. Albright, C. D., E. Klem, A. A. Shah and P. Gallagher. 2005. Breast cancer cell targeted oxidative stress: Enhancement of cancer uptake of conjugated linoleic acid, activation of p53, and inhibition of proliferation. Exp. Molec. Pathol. 79:118-125. https://doi.org/10.1016/j.yexmp.2005.05.005
  2. AOAC. 1995. Official methods of analysis (15th ed.). Association of Official Analytical Chemists, Washington, DC.
  3. Choi, N. J., J. Y. Imm, S. J. Oh, B. C. Kim, H. J. Hwang and Y. J. Kim. 2005. Effect of pH and oxygen on conjugated linoleic acid (CLA) production by mixed rumen bacteria from cows fed high concentrate and high forage diets. Anim. Feed Sci. Technol. 123-124:643-653. https://doi.org/10.1016/j.anifeedsci.2005.04.054
  4. Bell, J. A. and J. J. Kennelly. 2000. Producing conjugated fatty acid enriched milk through practical dairy nutrition. Conference abstract:8th World Congress on Clinical Nutrition, Bankok, Thailand.
  5. Ashes, J. R., B. D. Siebert, S. K. Gulati, A. Z. Cuthbertson and T. W. Scott. 1992. Incorporation of n-3 fatty acids of fish oil into tissue and serum lipids of ruminants. Lipids 27:629-631. https://doi.org/10.1007/BF02536122
  6. Choi, S. H., K. W. Lim, H. G. Lee, Y. J. Kim and M. K. Song. 2007. Supplementation effects of $C_{18:2}\;or\;C_{18:3}$ rich oils on formations of CLA and TVA, and lipogenesis in adipose tissues of sheep. Asian-Aust. J. Anim. Sci. 20:1417-1423. https://doi.org/10.5713/ajas.2007.1417
  7. Dhiman, T. R., G. R. Anand, L. D. Satter and M. W. Pariza. 1996. Conjugated linoleic acid content of milk from cows fed different diets. J. Dairy Sci. 79(Suppl. 1):137(Abstr.)
  8. Cook, M. E., C. C. Miller, Y. Park and M. Pariza. 1993. Immune modulation by altered nutrient metabolism: Nutritional control of immune induced growth depression. Poult. Sci. 72:1301-1305. https://doi.org/10.3382/ps.0721301
  9. Chung, T. Y., M. K. Song, C. S. Moon, C. M. Kim, D. I. Kim and J. S. Sim. 1996. Effect of flaxseed supplementation on the nutrient utilizing efficiency, milk productivity and fatty acid composition of milk by lactating Holstein cows. Kor. J. Nutr. Feed. 20:443-452.
  10. Chouinard, P. Y., L. Corneau, M. L. Kelly, J. M. Griinari and D. E. Bauman. 1998. Effect of dietary manipulation on milk conjugated linoleic acid concentrations. J. Dairy Sci. 81(Suppl. 1):233(Abstr.).
  11. Lee, K. N., D. Kritchevsky and M. W. Pariza. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25. https://doi.org/10.1016/0021-9150(94)90034-5
  12. Grummer, R. R. 1991. Effect of feed on the composition of milk fat. J. Dairy Sci. 74:3244-3251. https://doi.org/10.3168/jds.S0022-0302(91)78510-X
  13. Kobayashi, Y., M. Wakita and S. Hoshino. 1992. Effects of the ionophore salinomycin on nitrogen and long-chain fatty acid profiles of digesta in the rumen and the duodenum of sheep. Anim. Feed Sci. Technol. 36:670 https://doi.org/10.1016/0377-8401(92)90087-M
  14. Fellner, V., F. D. Sauer and J. K. Kramer, 1997. Effect of nigericin, monensin, and tetranasin on biohydrogenation in continuous flow-through ruminal fermenters. J. Dairy Sci. 80:921-928. https://doi.org/10.3168/jds.S0022-0302(97)76015-6
  15. Dhiman, T. R., L. D. Satter, M. W. Pariza, M. P. Galli, K. Albright and M. X. Tolasa. 2000. Conjugated linoleic acid (CLA) content of milk from cows offered diets rich in linoleic and linolenic acid. J. Dairy Sci. 83:1016-1027. https://doi.org/10.3168/jds.S0022-0302(00)74966-6
  16. Fawcett, J. K. and J. E. Scott. 1960. A rapid and precise method for the determination of urea. J. Clin. Pathol. 13:156-163. https://doi.org/10.1136/jcp.13.2.156
  17. Loor, J. J., K. Ueda, A. Ferlay, Y. Chilliard and M. Doreau. 2005. Intestinal flow and digestibility of trans fatty acids and conjugated linoleic acids (CLA) in dairy cows fed a highconcentrate diet supplemented with fish oil, linseed oil, or sunflower oil. Anim. Feed Sci. Technol. 119:203-225. https://doi.org/10.1016/j.anifeedsci.2005.01.001
  18. Lepage, G. and C. C. Roy. 1986. Direct transesterification of all classes of lipid in a one-step reation. J. Lipid Res. 27:114-121.
  19. SAS. 1985. SAS User's Guide: Statistical Analysis Systems Institute, Inc., Cary, NC.
  20. Wang, J. H. and M. K. Song. 2003. pH affects the in vitro formation of cis-9, trans-11 CLA and trans-11 Octadecenoic acid by ruminal bacteria when incubated with oilseeds. Asian-Aust. J. Anim. Sci. 16:1743-1748. https://doi.org/10.5713/ajas.2003.1743
  21. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fibre, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  22. Wang, J. H. and M. K. Song. 2001. Effect od sources and levels of carbohydrates on fermentation characteristics and hydrogenation of linoleic acid by rumen bacteria in vitro. Asian-Aust. J. Anim. Sci. 14:48-53. https://doi.org/10.5713/ajas.2001.48
  23. The British Nutrition Foundation 1992. Sources of unsaturated fatty acids in the diet. In: The report of the British Nutrition Foundation's task force. Unsaturated fatty acids: nutritional and physiological significance, London, UK: Chapman and Hall, pp. 6-11.
  24. Sauer, F. D., V. Fellner, R. Kinsman, J. K. G. Kramer, H. A. Jackson, A. J. Lee and S. Chen. 1998. Methane output and lactation response in Holstein cattle with monensin or unsaturated fat added to the diet. J. Anim. Sci. 76:906-914. https://doi.org/10.2527/1998.763906x
  25. Steel, R. G. D. and J. H. Torrie. 1980. Principles and procedures of statistics. Mcgraw Hill Book Co., NY.
  26. Whigham, L. D., A. Higbee, D. E. Bjorling, Y. H. Park, M. W. Pariza and M. E. Cook. 2002. Decreased antigen-induced eicosanoid release in conjugated linoleic acid-fed guinea pigs. Am. J. Physiol. Intergrative Comp. Physiol. 282(4):R1104-R1112. https://doi.org/10.1152/ajpregu.00075.2001
  27. Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221-225. https://doi.org/10.5713/ajas.2005.221
  28. Wang, J. H., M. K. Song, Y. S. Son and M. B. Chang. 2002. Effect of concentrate level on the formation of conjugated linoleic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseed in vitro. Asian-Aust. J. Anim. Sci. 15:1115-1120. https://doi.org/10.5713/ajas.2002.1115
  29. Wang, J. H., S. H. Choi, K. W. Lim, K. H. Kim and M. K. Song. 2006. Effect of mixed oil and monensin supplementation, and feeding duration of supplements on c9,t11-CLA contents in plasma and fat tissues of Korean native (Hanwoo) steers. Asian-Aust. J. Anim. Sci. 19:1464-1469. https://doi.org/10.5713/ajas.2006.1464

피인용 문헌

  1. 1. Infrared process monitoring of conjugated linoleic acid/styrene/butyl acrylate bulk and emulsion terpolymerization vol.133, pp.26, 2016, doi:10.5713/ajas.2008.80104

과제정보

연구 과제 주관 기관 : Rural Development Administration