Prion Protein Genotypes in Pakistani Goats

DOI QR코드

DOI QR Code

Babar, M.E.;Nawaz, M.;Nasim, A.;Abdullah, M.;Imran, M.;Jabeen, R.;Chatha, S.A.;Haq, A.U.;Nawaz, A.;Mustafa, H.;Nadeem, A.

  • 투고 : 2007.08.27
  • 심사 : 2008.02.27
  • 발행 : 2008.07.01

초록

The PCR-amplified prion protein (PrP) gene was sequenced to determine the frequency of scrapie-associated as well as novel PrP genotypes in 72 healthy goats representing five breeds. A total of six genotypes were detected, resulting from the three reported 143 (H/R), 154 (R/H) and 240 (S/P) and the two novel 39 (S/R) and 185 (I/F) amino acid polymorphisms. Of the four silent mutations 42 (a$\rightarrow$g), 138 (c$\rightarrow$t), 231 (c$\rightarrow$a) and 237 (g$\rightarrow$c) detected in this study, 237 (g$\rightarrow$c) is novel. A genotype (SIP/RFP) harboring three amino acid polymorphisms 39 (S/R), 185 (I/F) and 240 (S/P) was found in few goats. Although both scrapie-associated genotypes with 143 (H/R) and 154 (R/H) polymorphisms and others with 39 (S/R), 185 (I/F) and 240 (S/P) polymorphisms were present in the studied Pakistani goats, their frequency was lower than that of the wild-type genotype SHRIS/SHRIS (34.7%). These results emphasize the need for further sequencing of the PrP gene in a large number of goats representing the five studied breeds, so that overall PrP variability can be assessed in these breeds in research addressing future concerns about scrapie.

키워드

Prion Protein Genotypes;Scrapie;Goats;Pakistan

참고문헌

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