A T-cell type multicentric Lymphoma affecting central nervous system in a Cocker Spaniel dog

  • Kim, Ju-Won (College of Veterinary Medicine, Konkuk University) ;
  • Jung, Dong-In (College of Veterinary Medicine, Konkuk University) ;
  • Kang, Byeong-Teck (College of Veterinary Medicine, Konkuk University) ;
  • Yoo, Jong-Hyun (BK21 Program of Integrative Network system for Veterinarians in Basic Science, Industrial Animals and Preventive Medicines, Konkuk University) ;
  • Park, Chul (School of Veterinary Medicine, University of California) ;
  • Kim, Dae-Young (College of Veterinary Medicine, University of Missouri-Columbia) ;
  • Park, Hee-Myung (College of Veterinary Medicine, Konkuk University)
  • Accepted : 2008.09.17
  • Published : 2008.12.01


A 4-year-old female Cocker spaniel was presented with respiratory distress and abdominal distension. Pleural effusion, ascites, hepatosplenomegaly, and superficial lymphadenopathy were observed and multicentric lymphoma was diagnosed by cytological examination. Immunophenotyping of lymph node and bone marrow using polymerase chain reaction for antigen receptor rearrangement identified a stage V lymphoma originating from T-cell. Despite of systemic chemotherapy using L-asparagenase, vincristine, cyclophoaphamide and prednisolone, neurologic deficits came out and progressed. Cerebrospinal fluid analysis revealed neoplastic lymphocytic pleocytosis indicating central nervous system involvement of lymphoma. The postmortem diagnosis was confirmed based on the histology and imunohistochemistry.


  1. Fan TM, Kitchell BE. An update on diagnosing and treating canine lymphosarcoma. Vet Med 2002, 97, 58- 67
  2. Fournel-Fleury C. Ponce F, Felman P, Blavier A, Bonnefont C, Chabanne L, Marchal T. Cadore JL. Goy-Thollot I, Ledieu D, Ghernati I. Magnol JP. Canine T-cell lymphomas: a morphological, immunological. and clinical study of 46 new cases. Vet Pathol 2002, 39, 92-109 https://doi.org/10.1354/vp.39-1-92
  3. Graham JC. O'kede DA, Wallig MA. Oluoch AO. Lymphosarcoma causing acquired obstructive hydrocephalus in a dog. Can Vet J 1992, 33, 669-670
  4. Creenlee PG, Filippa DA, Quimby FW. Patnaik AK, Calvano SE, Matus RE, Kimmel M, Hurvitz AI, Liebuman PH. Lymphomas in dogs. A morphologic, immunologic, and clinical study. Cancer 1990, 66, 480-490 https://doi.org/10.1002/1097-0142(19900801)66:3<480::AID-CNCR2820660314>3.0.CO;2-X
  5. Cuija de Aresphaga A, Sehwendenwein I, Weissenbk H. Retrospective Study of 82 cases of canine lymphoma in Austria based on the working formulation and immunophenotyping. J Comp Pathol 2007, 136, 186-192 https://doi.org/10.1016/j.jcpa.2007.01.009
  6. Kraft SL, Gavin PR. Intracranial neoplasia. Clin Tech Small Anim Pract 1999, 14, 112-123 https://doi.org/10.1016/S1096-2867(99)80009-7
  7. Grindem CB. Page RL, Ammerman BE, Dreitschwerdt EB. Immunophenotypic comparison of blood and lymph node from dogs with lymphoma. Vet Clin Pathol 1998, 27, 16-20 https://doi.org/10.1111/j.1939-165X.1998.tb01075.x
  8. Ricci Lueas SR, Pereira Coelho BM, Marquezi ML, Franchini ML, Miyashiro SI. De Benedello Pozzi DH. Carmustine, vincristine. and prednisone in the treatment of canine lymphosarcoma. J Am Anim Hosp Assoc 2004, 40, 292-299 https://doi.org/10.5326/0400292
  9. pfaff AM, March PA, Fishman C. Acute bilateral trigeminal neuropathy associated with nervous system lymphosarcoma in a dog. J Am Anim Hosp Assoc 2000, 36, 57-61 https://doi.org/10.5326/15473317-36-1-57
  10. Dobson J . Classification of canine lymphoma: a step forward. Vet J 2004, 167, 125-126 https://doi.org/10.1016/j.tvjl.2003.09.017
  11. Dbaliwal RS. Reed AL, Kilehell BE. Multicentric lymphosarcoma in a dog with multiple-site skeletal involvement. Vet Radiol Ultrasound 2001, 42, 38-41 https://doi.org/10.1111/j.1740-8261.2001.tb00901.x
  12. Valerius KD, Ogilvie GK, Mallinckrodt CII. Getzy DM. Doxorubicin alone or in combination with asparaginase. followed by cyclophosphamide. vincristine, and prednisone for treatment of multicentric lymphoma in dogs: 121 cases (1987-1995). J Am Vet Med Assoc 1997, 210, 512-516
  13. Bohn AA, Wills TB, West CL, Tucker RL, Bagley RS. Cerebrospinal fluid analysis and magnetic resonance imaging in the diagnosis of neurologic disease in dogs: a rctrospetive study. Vct Clin Pathol 2006, 35, 315-320 https://doi.org/10.1111/j.1939-165X.2006.tb00138.x
  14. Keller RL. Avery AC, Burnett RC, Walton JA, Olver CS. Detection of neoplastic Iymphocytes in peripheral blood of dogs with lymphoma by polymerase chain reaction for antigen recptor gene rearrangement. Vet Clin Pathol 2004, 33, 145-149 https://doi.org/10.1111/j.1939-165X.2004.tb00364.x
  15. Owen LN. TNM Classification of Tumors in Domestic Animals. 1st ed. p. 34. World Health Organization. Geneva, 1980
  16. Couto CG, Cullen J, Pedroia V. Thrrel JM. Central nervous system lymphosarcoma in the dog. J Am Vet Med Assoc 1984, 184, 809-813
  17. Ettinger SN. Principles of treatincnl for eanine lymphoma. Clin Tech Small Anim Pract 2003, 18, 92-97 https://doi.org/10.1053/svms.2003.36622
  18. Jagielski D, Lechowski R. Hoffmann-Jagielska M, Winiarttyk S. A retrospective Study of the incidence and prognostic factors of multicentric lymphoma in dogs ( 1998-2000). J Vet Med A Physiol Pathol Clin Med 2002, 49, 419-424 https://doi.org/10.1046/j.1439-0442.2002.00458.x
  19. MacKintosh FR, Colby TV. Podolsky WJ, Burke JS, Hoppe RT. Rosenrelt FP. Rosenberg SA, Kaplan HS. Central nervous system involvement in non-Hodgkin's lymphoma: an analysis of 105 cases. Cancer 1982, 49, 586-595 https://doi.org/10.1002/1097-0142(19820201)49:3<586::AID-CNCR2820490331>3.0.CO;2-C
  20. Long SN. Johnston PE. Anderson TJ. Primary T-cell lymphoma of the central nervous system in a dog. J Am Vet Med Assoc 2001, 218, 719-722 https://doi.org/10.2460/javma.2001.218.719
  21. Ferrer L, Fondevila D, Rabanal R, Tarres J. Ramis A. Immunohistochemical detection of CD3 antigen (pan T marker) in canine lymphomas. J Vet Diag Invets 1993, 5, 616-620 https://doi.org/10.1177/104063879300500420