Asian-Australasian Journal of Animal Sciences (아세아태평양축산학회지)

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Maintenance of Sperm Characteristics and In vitro Developmental Rate of Embryos against Oxidative Stress through Antioxidants in Pig

  • Jang, H.Y. (College of Animal Resources Science, Kangwon National University) ;
  • Kong, H.S. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Oh, J.D. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Park, B.K. (College of Animal Resources Science, Kangwon National University) ;
  • Yang, B.K. (College of Animal Resources Science, Kangwon National University) ;
  • Jeon, G.J. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Lee, H.K. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University)
  • Received : 2005.07.05
  • Accepted : 2007.09.28
  • Published : 2008.03.01

Abstract

Oxidative stress is one of the major causes of failure of in vitro storage of boar semen. Reactive oxygen species (ROS) are one of the important mediators of oxidative stress during in vitro storage of boar semen. Our study examined the effects of taurine on sperm characteristic and on in vitro developmental embryos during in vitro storage of boar semen for 7 days. Semen was randomly aliquoted into 3 centrifuge tubes and treated with different concentrations of taurine (25-100 mM). The characteristics of boar sperm were analyzed for motility by light microscopy, viability by using a Makler counting chamber and membrane integrity by a hypoosmotic swelling test (HOST). The percentages of motile spermatozoa in taurine groups after 5 days were significantly higher compared to the control. Sperm viability in the control was lower than in taurine groups after 7 days irrespective of different taurine concentration. In the hyoosmotic swelling test (HOST), significantly higher results were obtained in taurine groups after 3 days. Also, the developmental rates of IVM/IVF porcine embryos from semen treated with pyruvate and taurine were significantly increased when compared with the control (p<0.05). These results indicate that supplementation of taurine as an antioxidant in boar semen extender can improve the semen quality.

Keywords

Reactive Oxygen Species (ROS);Boar Semen;Taurine;Embryo Antioxidant;Sperm Characteristic

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Acknowledgement

Supported by : Hankyong National University

References

  1. Wright, C. E., T. T. Lin, J. A. Syurman and G. E. Gaull. 1985. Taurine scavenges oxidized chlorine in biological systems. In; Taurine: biological actions and clinical perspectives (Ed. S. S. Oja et al.), Alan. R. Liss. Inc., New York, pp. 137-147.
  2. Van Winkle, L. J. and H. R. Dickinson. 1995. Differences in amino acid content of preimplantation mouse embryos that develop in vitro vs. in vivo. In vitro effects of 5 amino acids that are abundant in oviductal secretions. Biol. Reprod. 52:96-104. https://doi.org/10.1095/biolreprod52.1.96
  3. Wright, C. E., H. H. Tallan, Y. Y. Lin and G. E. Gaull. 1986. Taurine: biological update. Ann. Rev. Biolchem. 55:427-453. https://doi.org/10.1146/annurev.bi.55.070186.002235
  4. Stubbs, C. D. and A. D. Smith. 1984. The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochim. Biophys. Acta. 779(1):89-137. https://doi.org/10.1016/0304-4157(84)90005-4
  5. Sun, J. G., A. Jurisicova and R. F. Casper. 1997. Detection of deoxyribonucleic acid fragmentation in human sperm; Correction with fertilization in vitro. Biol. Reprod. 56:602-607. https://doi.org/10.1095/biolreprod56.3.602
  6. Jang, H.Y., H. S. Kong, S. C. K. Park, J. D. Oh, S. G. Lee, H. T. Cheong, J. T. Kim, S. J. Lee, B. K. Yang and H. K. Lee. 2006. Effects of taurine on spermCharacteristics during in vitro storage of boar semen. Asian-Aust. J. Anim. Sci. 19:1561-1565. https://doi.org/10.5713/ajas.2006.1561
  7. Huxtable, R. J. 1992. Physiological actions of taurine. Physiol. Rev. 72:101-163. https://doi.org/10.1152/physrev.1992.72.1.101
  8. Fraga, G. G., P. A. Motchnik, M. K. Shigenaga, J. G. Helbrock, R. A. Jacob and B. Ames. 1991. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc. Natl. Acad. Sci. USA 88:11003-11006. https://doi.org/10.1073/pnas.88.24.11003
  9. Halliwell, B. and O. I. Aruoma. 1991. DNA damage by oxygenderived species. Its mechanism and measurement in mammalian systems. FEBS Lett. 281:9-19. https://doi.org/10.1016/0014-5793(91)80347-6
  10. Chen, C. S., H. T. Chao, R. L. Pan and Y. H. Wei. 1997. Hydroxyl radical-induced decline in motility and increase in lipid peroxidation and DNA modification in human sperm. Biochem. Mol. Biol. Int. 43:291-303.
  11. Dumoulin, J. C. M., J. L. H. Evers and M. Bras. 1992. Positive effect of taurine on preimplantation development of mouse embryos in vitro. J. Reprod. Fertil. 94:373-380. https://doi.org/10.1530/jrf.0.0940373
  12. Aitken, R. J., M. Paterson, H. Fisher, D. W. Buckingham and M. van Duin. 1995. Redox regulation of tyrosine phosphorylation in human spermatozoa and its role in the control of human sperm function. J. Cell. Sci. 108:2017-2025.
  13. Cerolini, S., A. Maldjian, P. Surai and R. Noble. 2000. Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim. Reprod. Sci. 58(1- 2):99-111. https://doi.org/10.1016/S0378-4320(99)00035-4
  14. Chesney, R. W. 1985. Taurine: its biological role and clinical implications. Adv. Pediatr. 32:1-42.
  15. Alvarez, J. G. and B. T. Storey. 1995. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol. Reprod. Dev. 42(3):334-46. https://doi.org/10.1002/mrd.1080420311
  16. Aitken, J. R., J. S. Clarkson and S. Fishel. 1989. Generation of reactive oxygen species, lipid peroxidation and human sperm function. Biol. Reprod. 40:183-197.
  17. Aitken, R. J. 1997. Molecular mechanisms regulating human sperm function. Mol. Hum. Reprod. 3:169-173. https://doi.org/10.1093/molehr/3.3.169
  18. Alexopoulos, C., C. Boscos, P. H. Saratsis, C. Saoulidis and S. Kyriakis. 1996. The effect of storage time and number of spermatozoa per insemination dose on semen characteristics and fertilizing capacity of boar semen diluted with Beltsville Thaw Solution (BTS) extender. Anim. Sci. 62:599-604. https://doi.org/10.1017/S1357729800015150
  19. Redmond, H. P., J. H. Wang and D. BouchierHayes. 1996. Taurine attenuates nitric oxide and reactive oxygen intermediatedependent hepatocyte injury. Arch. Surg. 131:1280-1287. https://doi.org/10.1001/archsurg.1996.01430240034004
  20. Maxwell, W. M. C. and L. A. Johnson. 1997. Membrane status of boar spermatozoa after colling or cryopreservation. Theriogenol. 48:209-219. https://doi.org/10.1016/S0093-691X(97)84068-X
  21. Ogasawara, M., T. Nakamura and I. Koyama. 1993. Reactivity of taurine with aldehydes and its physiological role. Chem. Pharm. Bull. 41:2172-2175. https://doi.org/10.1248/cpb.41.2172
  22. Laforest, J. P. and A. Allard. 1996. Comparison of four extenders for long-term storage of fresh boar semen: III. International conference on boar semen preservation. Reprod. Domest. Anim. 311:275-276.
  23. Li, J. and R. H. Foote. 1993. Culture of rabbit zygotes into blastocysts in protein free medium with one to twenty percent oxygen. J. Reprod. Fertil. 98:163-167. https://doi.org/10.1530/jrf.0.0980163
  24. Kim, J. Y., S. B. Kim, M. C. Park, H. Park, Y. S. Park, H. D. Park, J. H. Lee and J. M. Kim. 2007. Addition of macromolecules to PZM-3 culture medium on the development and hatching of in vitro porcine embryos. Asian-Aust. J. Anim. Sci. 20:1820- 1826. https://doi.org/10.5713/ajas.2007.1820
  25. Korniewicz, D., B. Fabianczyk and Z. Smorag. 1996. The survival rate and fertilizing capacity of boar semen diluted with different diluents: III. International conference on boar semen preservation. Reprod. Domest. Anim. 311:273-274.
  26. de Lamirande, E. and C. Gagnon. 1994. Reactive oxygen species (ROS) and reproduction. Adv. Exp. Med. Biol. 366:185-197. https://doi.org/10.1007/978-1-4615-1833-4_14