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Maintenance of Sperm Characteristics and In vitro Developmental Rate of Embryos against Oxidative Stress through Antioxidants in Pig

  • Jang, H.Y. (College of Animal Resources Science, Kangwon National University) ;
  • Kong, H.S. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Oh, J.D. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Park, B.K. (College of Animal Resources Science, Kangwon National University) ;
  • Yang, B.K. (College of Animal Resources Science, Kangwon National University) ;
  • Jeon, G.J. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University) ;
  • Lee, H.K. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University)
  • Received : 2005.07.05
  • Accepted : 2007.09.28
  • Published : 2008.03.01

Abstract

Oxidative stress is one of the major causes of failure of in vitro storage of boar semen. Reactive oxygen species (ROS) are one of the important mediators of oxidative stress during in vitro storage of boar semen. Our study examined the effects of taurine on sperm characteristic and on in vitro developmental embryos during in vitro storage of boar semen for 7 days. Semen was randomly aliquoted into 3 centrifuge tubes and treated with different concentrations of taurine (25-100 mM). The characteristics of boar sperm were analyzed for motility by light microscopy, viability by using a Makler counting chamber and membrane integrity by a hypoosmotic swelling test (HOST). The percentages of motile spermatozoa in taurine groups after 5 days were significantly higher compared to the control. Sperm viability in the control was lower than in taurine groups after 7 days irrespective of different taurine concentration. In the hyoosmotic swelling test (HOST), significantly higher results were obtained in taurine groups after 3 days. Also, the developmental rates of IVM/IVF porcine embryos from semen treated with pyruvate and taurine were significantly increased when compared with the control (p<0.05). These results indicate that supplementation of taurine as an antioxidant in boar semen extender can improve the semen quality.

Keywords

Reactive Oxygen Species (ROS);Boar Semen;Taurine;Embryo Antioxidant;Sperm Characteristic

Acknowledgement

Supported by : Hankyong National University

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