Variation of Characteristics of Nonstoichiometric Apatite Induced by Sodium Salt

나트륨염에 의한 비양론적 인회석의 특성 및 SaOS-2 세포반응에 미치는 영향에 관한 연구

  • Jung, Jae-Young (Laboratory of Biointerfaces & Tissue Engineering Research, Department of Chemical Engineering, Institute of Tissue Regeneration Engineering, Dankook University) ;
  • Han, Juyun (Laboratory of Biointerfaces & Tissue Engineering Research, Department of Chemical Engineering, Institute of Tissue Regeneration Engineering, Dankook University) ;
  • Choi, Sun-Mi (Laboratory of Biointerfaces & Tissue Engineering Research, Department of Chemical Engineering, Institute of Tissue Regeneration Engineering, Dankook University) ;
  • Lee, Woo-Kul (Laboratory of Biointerfaces & Tissue Engineering Research, Department of Chemical Engineering, Institute of Tissue Regeneration Engineering, Dankook University)
  • 정재영 (생체계면조직공학 연구실, 화학공학과, 조직재생공학연구소, 단국대학교) ;
  • 한주연 (생체계면조직공학 연구실, 화학공학과, 조직재생공학연구소, 단국대학교) ;
  • 최선미 (생체계면조직공학 연구실, 화학공학과, 조직재생공학연구소, 단국대학교) ;
  • 이우걸 (생체계면조직공학 연구실, 화학공학과, 조직재생공학연구소, 단국대학교)
  • Received : 2008.04.11
  • Accepted : 2008.05.03
  • Published : 2008.06.10

Abstract

The purpose of this work is to develope sodium-containing nonstoichiometric apatitic coatings on solid substrate. The apatitic coatings prepared at different concentrations of sodium salt indicated that the presence of sodium ions exerted significant effects on the physicochemical properties of the apatitic coating including surface morphology, chemical state, and Ca/P ratio. The variation of these properties was sustained up to 0.01 mM of sodium ion concentration. The ratio of calcium to phosphorus was varied from 2.18 to 2.03 which indicated the apatitic coating prepared in this study was calcium-rich nonstoichiometric apatite. The structure of all the samples appeared to be low crystalline. In the presence of sodium ion within the apaptitic coating, the adhesion of human osteoblast-like SaOS-2 cells was significantly promoted. On the other hand, the proliferation of the cells on the apatitic coatings was decreased with the increase of sodium ions. This reverse response of SaOS-2 cells indicates that the interaction between SaOS-2 and apatitic surface triggered considerable changes in intracellular mechanisms including cellular signal transductions.

Acknowledgement

Supported by : 단국대학교

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