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Identification of Differentially Expressed Proteins at Four Growing Stages in Chicken Liver

  • Lee, K.Y. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Jung, K.C. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Jang, B.G. (Livestock Research Center, National Institute of Subtropical Agriculture, RDA) ;
  • Choi, K.D. (The Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University)
  • Received : 2007.11.08
  • Accepted : 2008.04.23
  • Published : 2008.10.01

Abstract

Because of high growth rate and large deposition of fat in the abdomen, the chicken has been used as a model organism for understanding lipid metabolism, fattening and growing. In this study, differentially expression of proteins in chicken liver, one of the important organs for lipid metabolism, has been investigated at four different growing stages. After separation of proteins using two-dimensional electrophoresis (2-DE), more than 700 protein spots were detected. Among them, 13 growing stage specific proteins in chicken liver were selected and further investigated by matrix-assisted laser adsorptions ionization-time of flight mass spectrometry (MALDI-TOF MS). Of these, 12 proteins were matched to existing proteins based on a database search. The identified fat-related proteins in this study were fatty acid synthase (FASN) and malic enzyme (ME1). These proteins were more highly expressed at week 32 than at other weeks. In order to confirm the differential expression, one of the proteins, FASN, was confirmed by western blotting. The identified proteins will give valuable information on biochemical roles in chicken liver, especially for lipid metabolism.

Keywords

Chicken Liver;Different Growing Stages;MALDI-TOF MS;2-DE

Acknowledgement

Supported by : Rural Development Administration

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