Identification of Differentially Expressed Proteins at Four Growing Stages in Chicken Liver

  • Lee, K.Y. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Jung, K.C. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University) ;
  • Jang, B.G. (Livestock Research Center, National Institute of Subtropical Agriculture, RDA) ;
  • Choi, K.D. (The Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University)
  • Received : 2007.11.08
  • Accepted : 2008.04.23
  • Published : 2008.10.01


Because of high growth rate and large deposition of fat in the abdomen, the chicken has been used as a model organism for understanding lipid metabolism, fattening and growing. In this study, differentially expression of proteins in chicken liver, one of the important organs for lipid metabolism, has been investigated at four different growing stages. After separation of proteins using two-dimensional electrophoresis (2-DE), more than 700 protein spots were detected. Among them, 13 growing stage specific proteins in chicken liver were selected and further investigated by matrix-assisted laser adsorptions ionization-time of flight mass spectrometry (MALDI-TOF MS). Of these, 12 proteins were matched to existing proteins based on a database search. The identified fat-related proteins in this study were fatty acid synthase (FASN) and malic enzyme (ME1). These proteins were more highly expressed at week 32 than at other weeks. In order to confirm the differential expression, one of the proteins, FASN, was confirmed by western blotting. The identified proteins will give valuable information on biochemical roles in chicken liver, especially for lipid metabolism.


Chicken Liver;Different Growing Stages;MALDI-TOF MS;2-DE


Supported by : Rural Development Administration


  1. Goodridge, A. G. 1968. The effect of starvation and starvation followed by feeding on enzyme activity and the metabolism of [U-14C]glucose in liver from growing chicks. Biochem. J. 108:667-673.
  2. Goodridge, A. G. 1973. Regulation of fatty acid synthesis in isolated hepatocytes prepared from the livers of neonatal chicks. J. Biol. Chem. 248:1924-1931.
  3. Gygi, S. P., Y. Rochon, B. R. Franza and R. Aebersold. 1999. Correlation between protein and mRNA abundance in yeast. Mol. Cell Biol. 19:1720-1730.
  4. Hillier, L. W., W. Miller, E. Birney, W. Warren, R. C. Hardison, C. P. Ponting, P. Bork, D. W. Burt, M. A. Groenen and M. E. Delany. 2004. Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432:695-716.
  5. Arthur, J. A. and G. A. A. Albers. 2003. Industrial perspective on problems and issues associated with poultry breeding. In: Poultry genetics, breeding and biotechnology (Ed. W. M. Muir and S. E. Aggrey). pp. 1-12. CABI Publishing, Wallingford, UK.
  6. Dettmer, K. and B. D. Hammock. 2004. Metabolomics-a new exciting field within the "omics" sciences. Environ Health Perspect. 112:A396-397.
  7. Ding, S. T., Y. K. Ko, B. R. Ou, P. H. Wang, C. L. Chen, M. C. Huang, Y. P. Lee, E. C. Lin, C. F. Chen, H. W. Lin and W. T. K. Cheng. 2008. The expression of genes related to egg production in the liver of Taiwan country chickens. Asian-Aust. J. Anim. Sci. 21:19-24.
  8. Doherty, M. K., L. McLean, J. R. Hayter, J. M. Pratt, D. H. Robertson, A. El-Shafei, S. J. Gaskell, R. J. Beynon. 2004. The proteome of chicken skeletal muscle: changes in soluble protein expression during growth in a layer strain. Proteomics. 4:2082-2093.
  9. Fiehn, O. 2002. Metabolomics-the link between genotypes and phenotypes. Plant. Mol. Biol. 48:155-171.
  10. Futcher, B., G. I. Latter, P. Monardo, C. S. McLaughlin and J. I. Garrels. 1999. A sampling of the yeast proteome. Mol. Cell Biol. 19:7357-7368.
  11. Kanai, M. 1989. Ultrastructural and biochemical studies of lipolysis by lipolysosomes in chick hepatocytes. Cell Tissue Res. 255:559-565.
  12. Kanai, M., N. Watari, T. Soji and E. Suqawara. 1994. Formation and accumulation of lipolysosomes in developing chick hepatocytes. Cell Tissue Res. 275:125-132.
  13. Kanai, M., T. Soji, D. C. Herbert. 1997. Biogenesis and function of lipolysosomes in developing chick hepatocytes. Microsc. Res. Tech. 39:444-452.<444::AID-JEMT7>3.0.CO;2-G
  14. Kelly, V. C., S. Kuy, D. J. Palmer, Z. Xu, S. R. Davis and G. J. Cooper. 2006. Characterization of bovine seminal plasma by proteomics. Proteomics 6:5826-5833.
  15. Kuhajda, F. P. 2000. Fatty-acid synthase and human cancer:New perspectives on its role in tumor biology. Nutr. 16:202-208.
  16. Naaby-Hansen, S., M. D. Waterfield and R. Cramer. 2001. Proteomics-post-genomic cartography to understand gene function. Trends Pharmacol. Sci. 22:376-384.
  17. Pandey, A. and M. Mann. 2000. Proteomics to study genes and genomes. Nature 405:837-846.
  18. Park, Y. D., S. Y. Kim, H. S. Jang, E. Y. Seo, J. H. Namkung, H. S. Park, S. Y. Cho, Y. K. Paik and J. M. Yang. 2004. Towards a proteomic analysis of atopic dermatitis: A two-dimensionalpolyacrylamide gel electrophoresis/mass spectrometric analysis of cultured patient-derived fibroblasts. Proteomics 4:3446-3455.
  19. Smith, S. 1994. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASNEB J. 8:1248-1259.
  20. Smith, S., A. Witkowski and A. K. Joshi. 2003. Structural and functional organization of the animal fatty acid synthase. Prog. Lipid Res. 42:289-317.
  21. Stern, C. D. 2004. The chick embryo - Past, present and future as a model system in developmental biology. Mech. Dev. 121:1011-1013.
  22. Stern, C. D. 2005. The chick: A great model system becomes even greater. Dev. Cell 8:9-17.
  23. Jung, K. C., W. Y. Jung, Y. J. Lee, S. L. Yu, K. D. Choi, B. G. Jang, J. T. Jeon and J. H. Lee. 2007. Comparisons of chicken muscles between layer and broiler breeds using proteomics. Asian-Aust. J. Anim. Sci. 20:307-312.
  24. Kim, N. K., J. H. Lim, M. J. Song, O. H. Kim, B. Y. Park, M. J. Kim, I. H. Hwang and C. S. Lee. 2007. Developmental Proteomic Profiling of Porcine Skeletal Muscle during Postnatal Development. Asian-Aust. J. Anim. Sci. 20:1612-1617.
  25. Witkowski, A., V. S. Rangan, Z. I. Randhawa, C. M. Amy and S. Smith. 1991. Structural organization of the multifunctional animal fatty acid synthase. Eur. J. Biochem. 198:571-579.

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