Survey of extended-spectrum β-Lactamase (ESBL) in pathogenic Escherichia coli isolated from poultry in Korea

국내 가금유래 병원성 대장균의 extended-spectrum β-lactamase(ESBL) 특성 조사

  • Sung, Myung-Suk (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Jin-Hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Cho, Jae-Keun (Health & Environmental Research Institute) ;
  • Seol, Sung-Yong (Kyungpook National University School of Medicine) ;
  • Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University)
  • 성명숙 (경북대학교 수의과대학) ;
  • 김진현 (경북대학교 수의과대학) ;
  • 조재근 (대구시 보건환경연구원) ;
  • 설성용 (경북대학교 의학전문대학원) ;
  • 김기석 (경북대학교 수의과대학)
  • Accepted : 2008.08.28
  • Published : 2008.12.01

Abstract

This study was conducted to investigate incidence of extended-spectrum ${\beta}$-lactamase (ESBL) producing strains and characteristics of ESBL gene in pathogenic Escherichia coli isolated from poultry during the period from April 2003 to December 2005 in Korea. Among 203 isolates, 4 isolates (3 from broilers and 1 from layer) were confirmed as ESBL producing strains by double disk synergy test, polymerase chain reaction and sequencing for ${\beta}$-lactamase genes. $bla_{CTX-M-15}$ and $bla_{CMY-2}$ were detected in these 4 isolates and were transferred to recipient by conjugation, respectively. Also, these ESBL producing strains were associated with multiple drug resistance. In conclusion, these results exhibit incidence of CTX-M and CMY-2 ${\beta}$-lactamase in pathogenic E coli from poultry in Korea, and clinically important meaning in human. And they also suggest the needs for rapid and broad surveillance to monitor ESBL genes and R plasmid transferring resistant gene in poultry.

References

  1. 성명숙, 김진현, 하종수, 조재근, 설성용, 김기석. 가금 유래 벼원성대장균의 생화학적 성상 및 혈청형. 대한수의학회지 2009, 48, 145-151
  2. 식품의햑품안정청. 축산 항생제내성 및 항상제 사용 실태 조사. 식품의약품안정청, 서울, 2007
  3. 이계남, 김우주, 이연희. 국내 대학병원에서 분리된 Escherichia coli의 Extended-spectrum $\beta -Lactamase$ (ESBL) 현황. 미생물학회지 2004, 40, 295-301
  4. 이훈구. 부산 도축장에서 분리된 광범위 베타 락탐분해효소(Extended-Spedtrum $\beta -Lactamase$, ESBL) 생성 장내세군의 형별분류. 미생물학회지 2006, 42, 125-130
  5. 石井良和. 基質特伊性组擴張型 $\beta$ ラクタマーゼ(ESBL)生産菌. モダンメデイア 2007, 53, 8-14
  6. Bonnet R. Growing group of extended-spectrum$\beta -lactamases:$ the CTX-M enzymes. Antimicrob Agents Chemother 2004, 48, 1-14 https://doi.org/10.1128/AAC.48.1.1-14.2004
  7. Bou G, Oliver A, Martinez-Beltnin J . OXA-24, a novel class D $\beta -lactamases$ with carbapenemase activity in an Acinetobacter baumannii clinical strain. Antimicrob Agents Chemother 2000, 44, 1556-1561 https://doi.org/10.1128/AAC.44.6.1556-1561.2000
  8. Bradford PA. Extended-spectrum $\beta -lactamases$ in the 21st century: Characterization, epidemiology, and detection of this important resislance threat. Clin Microbiol Rev 2001, 14, 933-951 https://doi.org/10.1128/CMR.14.4.933-951.2001
  9. Bradley DE, Taylor DE, Cohen DR. Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. J Bacteriol 1980, 143, 1466-1470
  10. Bush K, Jacoby GA, Medeiros AA. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 1995, 39, 1211-1233 https://doi.org/10.1128/AAC.39.6.1211
  11. Danel F, Hall LM, Gur D, Akalin HE, Livermore DM. Transferable production of PER-1 $\beta -lactamases$ in Pseudomonas aeruginosa. J Antimicrob Chemother 1995, 35, 281-294 https://doi.org/10.1093/jac/35.2.281
  12. De Champs C, Sirot D, Chanal C, Bonnet R, Sirot J . A 1998 survey of extended-spectrum $\beta -lactamases$ in Enterobacteriaceae in France. The freneh study group. Antimicrob Agents Chemother 2000, 44, 3177-3179 https://doi.org/10.1128/AAC.44.11.3177-3179.2000
  13. Gniadkowski M, Palucha A, Grzesiowski P, Hryniewicz W. Outbreak of ceftazidime-resistant Klebsiella pneumoniae in a pediatric hospital in Warsaw, Poland: clonal spread of the TEM-47 extended-spectrum $\beta -lactamases$ (ESBL)-produeing strain and transfer of a plasmid carrying the SHV-5- Like ESBL-encoding gene. Antimicrob Agents Chemother 1998, 42, 3079-3085
  14. Gniadkowski M, Schneider I, Palucha A, Jungwirth R, Mikiewicz B, Bauernfeind A. Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaximehydrolyzing $\beta -lactamases$ that is closely related to the CTX-M-1/MEN-1 enzyme. Antimicrob Agents Chemother 1998, 42, 827-832
  15. Jeong SH, Bae lK, Kwon SB, Lee JH, Jung HI, Song JS, Jeong BC, Kim SJ, Lee SH. Investigation of extended-spectrum $\beta -lactamases$ produced by clinical isolates of Klebsiella pneumoniae and Escherichio coli in Korea. Lett Appl Microbiol 2004, 39, 41-47 https://doi.org/10.1111/j.1472-765X.2004.01537.x
  16. Jeong SH, Bae IK, Lee JH, Sohn SG, Kang GH, Jeon GJ, Kim YH, Jeong BC, Lee SH. Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. J Clin Microbiol 2004, 42, 2902-2906 https://doi.org/10.1128/JCM.42.7.2902-2906.2004
  17. Kim J , Kwon Y, Pai H, Kim JW, Cho DT. Survey of Klebsiella pneumoniae strains producing extended- spectrum beta-lactamases: prevalence of SHV-12 and SHV-2a in Korea. J Clin Microbiol 1998, 36, 1446-1449
  18. Kim J, Lim YM. Jeong VS, Seol SY. Occurrence of CTX-M-3, CTX-M-15, CTX-M-14, and CTX-M-9 extended-spectrum $\beta -lactamases$ in enterobacteriae clinical isolates in Korea. Antimicrob Agents Chemother 2005, 49, 1572-1575 https://doi.org/10.1128/AAC.49.4.1572-1575.2005
  19. Kim J, Lim YM, Rheem I, Lee Y, Lee JC, seol SY. Lee YC, Cho DT. CTX-M and SHV-12 $\beta -lactamases$ arc the most common extended-spectrum enzymes in clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 3 university hospitals within Korea. FEMS Microbiol Lett 2005, 245, 93-98 https://doi.org/10.1016/j.femsle.2005.02.029
  20. Kojima A, Ishii Y, Ishihara K, Esaki H, Asai T, Oda C, Tamura Y, Takahashi T, Yamaguchi K. $Extended-spectrum-\beta -lactamases-producing$ Escherichia coli strains isolated from farm animals from 1999 to 2002: report from the Japanese veterinary antimicrobial resistance monitoring program. Antimirob Agents Chemother 2005, 49, 3533-3537 https://doi.org/10.1128/AAC.49.8.3533-3537.2005
  21. Livermore DM. $\beta -lactamases$ in laboratory and clinical resistance. Clin Microbiol Rev 1995, 8, 557-584
  22. Naas T, Nordmann P. OXA-type $\beta -lactamases$. Curr Pharm Des 1999, 5, 865-879
  23. National CommiUee for Clinical Laboratory Standards (NCCLS). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved standard. 6th ed. NCCLS document M7-A6. NCCLS, Villanova, 2003
  24. Pai H. Choi EH, Lee HJ, Hong JY, Jaeoby GA. Identification of CTX-M-14 extended-spectrum $\beta -lactamases$ in clinical isolates of Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae in Korea. J Clin Microbiol 2001, 39, 3747-3749 https://doi.org/10.1128/JCM.39.10.3747-3749.2001
  25. Pai H, Lyu S, Lee JH. Kim J, Kwon Y, Kim JW, Choe KW. Survey of extended-spectrum $\beta -lactamases$ in clinical isolates of Escherichia coli and Klebsiella pneumoniac: prevalence of TEM-52 in Korea. J Clin Microbiol 1999, 37, 1758- 1763
  26. Philippon A, Arlet G, Jacoby GA. Plasmiddetermined AmpC-type $\beta -lactamases$. Antimicrob Agents Chemothcr 2002, 46, 1-11 https://doi.org/10.1128/AAC.46.1.1-11.2002
  27. Sturenburg E, Mack D. Extended-spectrum beta-lactamases: implications for the clinical microbiology laboralory, therapy, and infeclion control. J Infect 2003, 47, 273-295 https://doi.org/10.1016/S0163-4453(03)00096-3
  28. Tabalabaei RR, Nasirian A. Comparison of plasmid profile analysis, serotyping and antimicrobial resistance patterns of E. coli isolated from poultry in Tehran. Food Sci Biotechnol 2004, 13, 100-103
  29. Voogd CE, Schot CS, van Leeuwen WJ, van Klingeren B. Monitoring of antibiotic resistance in Shigellae isolated in the Netherlands 1984-1989. Eur J Clin Microbiol Infect Dis 1992, 11, 164-167 https://doi.org/10.1007/BF01967070
  30. World Health Organization (WHO). The medical impact of antimicrobial use in food animals. In: Report of a WHO meeting. WHO, Geneva, 1997
  31. World Health Organization (WHO). Use of quinolones in food animals and potential impact on human health. In: Report of a WHO Meeting. WHO, Geneva, 1998
  32. Yan JJ, Ko WC, Tsai SH. Wu HM, Jin YT, Wu JJ. Dissemination of CTX-M-3 and CMY-2 $\beta -Lactamases$ among Clinical isolates of Escherichia coli in Southern Taiwan. J Clin Microbiol 2000, 38, 4320-4325
  33. Zhou XY, Bordon F, Sirot D, Kitzis MD, Gutmann L. Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-I $\beta -lactamases$ conferring resistance to $\beta -lactamases$ inhibitors. Antimicrob Agents Chemother 1994, 38, 1085-1089 https://doi.org/10.1128/AAC.38.5.1085