Antimicrobial resistance and distribution of tetracycline resistance determinants in Escherichia coli isolated from aquatic birds

수생조류에서 분리한 대장균의 항균제 내성 및 Tetracycline 내성인자의 분포

  • Cho, Jae-Keun (Health & Environmental Research Institute) ;
  • Lee, Sang-Min (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University)
  • 조재근 (대구광역시 보건환경연구원) ;
  • 이상민 (경북대학교 수의과대학) ;
  • 김기석 (경북대학교 수의과대학)
  • Accepted : 2008.06.30
  • Published : 2008.12.01

Abstract

One hundred and sixty nine Escherichia (E.) coli strains isolated from fecal samples of aquatic birds in Geumho river basin and Dalseong park were tested by agar dilution method to determine their susceptibility patterns to 14 antimicrobial agents. The distribution of tetracycline resistance determinants (tetA, tetB, tetC, tetD and tetE) were also examined by PCR in 76 tetracycline-resistant ($TC^r$) E. coli isolates. The high resistance was observed in tetracycline, cephalothin and ampicillin (45.0~36.7%). Resistance of E. coli isolates derived from Dalseong park to tetracycline, cephalothin, ampicillin and streptomycin (65.7~44.8%) were significantly higher than those isolated from Geumho river basin (31.4~14.7%). About seventy percent (70.4%) of the strains isolated were resistant to one or more drugs tested. Thirty (39.5%) of 76 $TC^r$ E. coli isolates which were resistant to one or more drugs transferred all or a part of their resistance patterns to the recipient strain of E.coli J53 by conjugation. All of $TC^r$ E. coli isolates contained at least one or more of 5 tet genes examined. The most common genes found in these isolates were tetA (60.6%) and followed by tetB (7.9%) and tetC (1.3%). However, tetD and tetE were not found in any of the isolates tested. Twenty one (27.6%) of $TC^r$ E. coli isolates had two determinants, tetA/tetB (20 strains), tetA/tetC (1 strain). And two strains (2.6%) contained three determinants (tetA/tetB/tetC).

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