In vivo evaluation of preventive effect of Lactobacillus reuteri on porcine epidemic diarrhea in suckling piglets

  • Accepted : 2008.06.16
  • Published : 2008.08.01

Abstract

Lactic acid bacteria have been reported their beneficial roles on host including reduction of infectious diarrhea problems. In this study, preventive effect of Lactobacillus (L.) reuteri HY25101 and L. johnsonii HY25103 on porcine epidemic diarrhea virus (PEDV) was investigated in suckling piglets. Two groups of one day old PEDV naïve piglets were orally administered L. reuteri HY25101 and L. johnsonii HY25103 for three days respectively before challenge with lethal dose of PEDV. In second experiment, passive immunized one day old piglets using colostrums containing PEDV specific IgA were used. The survival rates of the L. reuteri HY25101 administered group were significantly higher than that of L. johnsonii HY25103 administered group and viral shedding was rapidly diminished in L. reuteri HY25101 administered group. Interestingly piglets born from the sow immunized with attenuated PEDV vaccine were not completely protected from PEDV challenge, however coadministeration of L. reuteri HY25101 and colostrums containing PEDV specific IgA were more effectively prevent PEDV infection. These results suggested that dietary treatment using L. reuteri HY25101 could reduce diarrheal problem and mortality rate caused by PEDV in suckling pigs. In addition, L. reuteri HY25101 could be used as one of effective compensation treatment with attenuated live vaccine for PED.

Acknowledgement

Supported by : Konkuk University

References

  1. Kaila M, Isolauri E, Soppi E, Virtanen E, Laine S, Arvilommi H. Enhancement of the circulating antibody secreting cell response in human diarrhea by a human Lactobacillus strain. Pediatr Res 1992, 32, 141-144 https://doi.org/10.1203/00006450-199208000-00002
  2. Ouwehand AC, Salminen S, Isolauri E. Probiotics: an overview of beneficial effects. Antonie Van Leeuwenhoek 2002, 82, 279-289 https://doi.org/10.1023/A:1020620607611
  3. Roth JA. The immune system. In: Straw BE, D'Allaire S, Mengeling WL, Taylor DJ (eds.). Disease of swine. 8th ed. pp.179-185, Iowa State University Press, Iowa, 1999
  4. Galdeano CM, Perdigon G. The probiotic bacterium Lactobacillus casei induces activation of the gut mucosal immune system through innate immunity. Clin Vaccine Immunol 2006, 13, 219-226 https://doi.org/10.1128/CVI.13.2.219-226.2006
  5. Kandler O, Stetter K, Kohl R. Lactobacillus reuteri sp. nov. a new species of heterofermentative lactobacilli. In. Cl: 264-269, Zbl. Bakt. Hyg. Abt. Orig., 1980
  6. Shornikova AV, Casas IA, Mykkanen H, Salo E, Vesikari T. Bacteriotherapy with Lactobacillus reuteri in rotavirus gastroenteritis. Pediatr Infect Dis J 1997, 16, 1103-1107 https://doi.org/10.1097/00006454-199712000-00002
  7. Fujisawa T, Benno Y, Yaeshima T, Mitsuoka T. Taxonomic study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and synonymy of Lactobacillus acidophilus group A3 (Johnson et al. 1980) with the type strain of Lactobacillus amylovorus (Nakamura 1981). Int J Syst Bacteriol 1992, 42, 487-491 https://doi.org/10.1099/00207713-42-3-487
  8. Isolauri E, Sutas Y, Kankaanpaa P, Arvilommi H, Salminen S. Probiotics: effects on immunity. Am J Clin Nutr 2001, 73, 444S-450S https://doi.org/10.1093/ajcn/73.2.444s
  9. Tlaskalova-Hogenova H, Stepankova R, Hudcovic T, Tuckova L, Cukrowska B, Lodinova-Zadnikova R, Kozakova H, Rossmann P, Bartova J, Sokol D, Funda DP, Borovska D, Rehakova Z, Sinkora J, Hofman J, Drastich P, Kokesova A. Commensal bacteria (normal microflora), mucosal immunity and chronic inflammatory and autoimmune diseases. Immunol Lett 2004, 93, 97-108 https://doi.org/10.1016/j.imlet.2004.02.005
  10. Wadstrom T, Andersson K, Sydow M, Axelsson L, Lindgren S, Gullmar B. Surface properties of lactobacilli isolated from the small intestine of pigs. J Appl Bacteriol 1987, 62, 513-520 https://doi.org/10.1111/j.1365-2672.1987.tb02683.x
  11. Shornikova AV, Casas IA, Isolauri E, Mykkanen H, Vesikari T. Lactobacillus reuteri as a therapeutic agent in acute diarrhea in young children. J Pediatr Gastroenterol Nutr 1997, 24, 399-404 https://doi.org/10.1097/00005176-199704000-00008
  12. Song DS, Oh JS, Kang BK, Yang JS, Moon HJ, Yoo HS, Jang YS, Park BK. Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain. Res Vet Sci 2007, 82, 134-140 https://doi.org/10.1016/j.rvsc.2006.03.007
  13. Rojas M, Conway PL. Colonization by lactobacilli of piglet small intestinal mucus. J Appl Bacteriol 1996, 81, 474-480
  14. Felley CP, Corthesy-Theulaz I, Rivero JL, Sipponen P, Kaufmann M, Bauerfeind P, Wiesel PH, Brassart D, Pfeifer A, Blum AL, Michetti P. Favourable effect of an acidified milk (LC-1) on Helicobacter pylori gastritis in man. Eur J Gastroenterol Hepatol 2001, 13, 25-29 https://doi.org/10.1097/00042737-200101000-00005
  15. Heyman M. Effect of lactic acid bacteria on diarrheal diseases. J Am Coll Nutr 2000, 19, 137S-146S https://doi.org/10.1080/07315724.2000.10718084
  16. Shroff KE, Meslin K, Cebra JJ. Commensal enteric bacteria engender a self-limiting humoral mucosal immune response while permanently colonizing the gut. Infect Immun 1995, 63, 3904-3913
  17. Pantoflickova D, Corthesy-Theulaz I, Dorta G, Stolte M, Isler P, Rochat F, Enslen M, Blum AL. Favourable effect of regular intake of fermented milk containing Lactobacillus johnsonii on Helicobacter pylori associated gastritis. Aliment Pharmacol Ther 2003, 18, 805-813 https://doi.org/10.1046/j.1365-2036.2003.01675.x