Variations in Conjugated Linoleic Acid Concentrations in Cows Milk, Depending on Feeding Systems in Different Seasons

  • Zunong, Maimaijiang (United Graduate School of Agricultural Sciences, Iwate University) ;
  • Hanada, Masaaki (Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine) ;
  • Aibibula, Yimamu (College of Pratacultural Engineering, Xinjiang Agricultural University) ;
  • Okamoto, Meiji (Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine) ;
  • Tanaka, Keiichi (Hokkaido Agricultural Laboratory for Business Development)
  • Received : 2008.03.07
  • Accepted : 2008.05.30
  • Published : 2008.10.01


Variations in conjugated linoleic acid (CLA) concentrations in Holstein dairy cows milk, depending on feeding systems in different seasons was investigated. Milk samples were collected from Holstein dairy cows, which either grazed for whole days (WG), only daylight hours (TG), or were offered a total mixed ration (TMR) and experienced no grazing (NG), from April to December of 2005. In April, November and December, the cows in TG and WG treatments received grass silage and some concentrate, while from May to October, the cows grazed on temperate pasture. The cows in NG treatment received the TMR throughout the season. The major fatty acid obtained in the pastures was linolenic acid. There was no significant difference in the pasture's linolenic acid concentrations from May to September, but there was a significant decrease in October. However, the linolenic acid concentrations obtained in the pasture were always much higher than those obtained from the TMR. Linoleic acid was also the major fatty acid in the TMR, but these concentrations were higher in the TMR than in the pasture. There was no significant difference in milk cis9trans11CLA (c9t11CLA) concentrations between the three feeding systems while the cows were fed on conserved pasture in April, November and December. Although c9t11CLA concentrations were lower in the TMR, it was found that the cows which grazed in fresh pasture experienced significantly higher concentrations of c9t11CLA in their milk than those which received only TMR. It was also found that cows in the WG treatment experienced higher c9t11CLA concentrations than those in the TG treatment. In the WG and TG treatments, c9t11CLA concentrations were highest in June, after which, they gradually decreased (p<0.01) until October. For the NG treatment, there was no significant change in the concentrations of c9t11CLA (p>0.05) with season. Overall, trans11C18:1 and c9t11CLA were greatly influenced by season, with higher variation in the WG treatment than in the TG treatment and no variation in the NG treatment.


Feeding System;Seasonal Change;Conjugated Linoleic Acid;Milk Fatty Acids Composition


  1. Schroeder, G. F., G. A. Gagliostro, F. Bargo, J. E. Delahoy and L. D. Muller. 2004. Effects of fat supplementation on milk production and composition of dairy cows on pasture. Livest. Prod. Sci. 86:1-18.
  2. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods of dietary fiber, and neutral detergent fiber and non-starch polysaccharides in nutrition. J. Dairy Sci. 74:3583-3597.
  3. West, W. J. 2003. Effects of heat-stress on production in dairy cattle. J. Dairy Sci. 86:2131-2144.
  4. Pollard, M. R., F. D. Gunstone, A. T. James and L. J. Morris. 1980. Desaturation of positional and geometric isomers of monoenoic fatty acid by microsomal perarations from rat liver. Lipids 15:306-314.
  5. Rearte, D. H. 2005. New insights into the nutritional value of grass. In: Utilisation of grazed grass in temperate animal systems: New insights into nutritional value of grass (Ed. J. J. P. N. Murphy). Wageningen Academic publishers, Netherlands, pp. 49-59
  6. Renaguli, M., Y. Keita, K. Mikio, O. Yuji and O. Masao. 2004. Increased levels of policosanol and very long-chain fatty acids in potato pulp fermented with rhizopus oryzae. Bios. Biot. Biochem. 68:2401-2404.
  7. SAS, 2002-2003. User's Guide: Statistics, Version 9.1.3 Edition. SAS Inst. Inc., Cary, NC, USA.
  8. Martin, S. A. and T. C. Jenkins. 2002. Factors affecting conjugated linoleic acid and trans-C18:1 fatty acid production by mixed ruminal bacteria. J. Anim. Sci. 80:3347-3352.
  9. Moate, P. J., W. Chalupa, T. C. Jenkins and R. C. Boston. 2004. A model to describe ruminal metabolism and intestinal absorption of long chain fatty acids. Anim. Feed Sci. Technol. 112:79-115.
  10. Moorby, J. M., R. T. Evans, N. D. Scollan, J. C. MacRace and M. K. Theodorou. 2006. Increasing concentration of water soluble carbohydrate in perennial ryegrass (Lolium perene): milk production from late-lactation dairy cows. Grass Forage Sci. 61:52-59.
  11. Morimoto, H. 1971. Experimental method for animal nutrition, Yokendo Publishers, Tokyo. pp. 293-422.
  12. Nakatsuji, H., Y. Nishimichi, M. Yayota, M. Takahashi, K. Ueda, S. Kondo and M. Okubo. 2006. Effects of grass height at the start of grazing on herbage intake and milk production under rotational grazing by lactating dairy cows. Grassland Sci. 52:175-180.
  13. Kay, J. K., T. R. Mackle, M. J. Auldist, N. A. Thomson and D. E. Bauman. 2004. Endogenous synthesis of cis-9, trans-11 conjugated linoleic acid in dairy cows fed fresh pasture. J. Dairy Sci. 87:236-378.
  14. Khanal, R. C. and T. R. Dhiman. 2007. Status of milk fat conjugated linoleic acid (CLA) in selected commercial dairies. Asian-Aust. J. Anim. Sci. 20(10):1525-1538.
  15. Kolver, E. S. and L. D. Muller. 1998. Performance and nutrient intake of high producing holstein cows consuming pasture or a total mixed ration. J. Dairy Sci. 81:1403-1411.
  16. Linnane, M. I., A. J. Breretonb and P. S. Giller. 2001. Seasonal changes in circadian grazing patterns of Kerry cows (Bos Taurus) in semi-feral conditions in Killarney National Park, Co. Kerry, Ireland. Appl. Anim. Behav. Sci. 71:277-292.
  17. Lock, A. L. and P. C. Garnsworthy. 2003. Seasonal variation in milk conjugated linoleic acid and 9-desaturase activity in dairy cows. Livest. Prod. Sci. 79:47-59.
  18. Bargo, F., J. E. Delahoy, G. F. Schroeder and L. D. Muller. 2006. Milk fatty acid composition of dairy cows grazing at two pasture allowances and supplemented with different levels and sources of concentrate. Anim. Feed Sci. Technol. 125:17-31.
  19. Bauman, D. E., L. H. Baumgard, B. A. Corl and J. M. Griinari. 2000. Biosynthesis of conjugated linoleic acid in ruminants. Proc. Am. Soc. Anim. Sci. 1999. online. Available: http:// proceedings/0937.pdf
  20. Brown, W., A. A. AbuGhazaleh and S. A. Ibrahim. 2008. Milk conjugated linoleic acid response to fish oil and linseed oil supplementation of grazing dairy cows. Asian-Aust. J. Anim. Sci. 21(5):663-670.
  21. Chilliard, Y., A. Ferlay, R. M. Mansbridge and M. Doreau. 2000. Ruminal milk fat plasticity: nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. Ann. Zootech. 49:181-205.
  22. Kay, J. K., J. R. Roche, E. S. Kolver, N. A. Thomson and L. H. Baumgard. 2005. A comparision between feeding systems (pasture and TMR) and the effect of vitamin E supplementation on plasma and fatty acid profiles in dairy cows. J. Dairy Res. 72:322-332.
  23. Aibibula, Y., A. Okine, M. Hanada, S. Murata, M. Okamoto and M. Goto. 2007. Effect of replacing rolled corn with potato pulp silage in grass silage-based diets on nitrogen utilization by steers. Asian-Aust. J. Anim. Sci. 20(8):1215-1221.

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