DOI QR코드

DOI QR Code

Effect of Dietary Conjugated Linoleic Acid (CLA) on Abdominal Fat Deposition in Yellow-feather Broiler Chickens and Its Possible Mechanism

  • Zhou, J. (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2007.03.14
  • Accepted : 2008.07.07
  • Published : 2008.12.01

Abstract

A total of 60 one-day-old Yellow-feather broiler chickens were allotted into treatment and control groups. The treatment group was fed with the diet supplemented with 3% conjugated linoleic acid (CLA) for 48 d, while control group was fed with the diet supplemented with 3% rapeseed oil. Chickens were slaughtered in each group at the age of 49 d, and the blood and the abdominal adipose tissue were sampled. Serum cLeptin and serum cAdiponectin were measured by ELISA. The total RNA was extracted from adipose tissue to measure the abundance of the chicken growth hormone receptor (cGHR), insulin-like growth factor 1 (cIGF-1), insulin-like growth factor I receptor (cIGF-IR), peroxisome proliferator-activated receptor gamma ($cPPAR{\gamma}$), cAdiponectin and cAdipoIR mRNA by RT-PCR using ${\beta}$-actin as an internal standard. Results showed that the CLA decreased the abdominal fat index by 20.93% (p<0.05). The level of serum cLeptin but not serum cAdiponectin was significantly increased by CLA treatment (p<0.05). CLA down-regulated the relative abundance of cGH-R mRNA and $cPPAR{\gamma}$ mRNA in abdominal adipose tissue by 24.74% (p<0.05) and 66.52% (p<0.01) respectively. However, no differences were found between CLA treatment group and control group (p>0.05) in the relative abundance of cIGF-1, cIGF-IR, cAdiponectin, and cAdipoIR mRNA in abdominal adipose tissue. The data suggested that CLA inhibited abdominal fat deposition in broiler chicken may be determined by decreasing the GHR available for GH, and by inhibiting the differentiation of preadipocytes via down-regulation of $PPAR{\gamma}$, but independent of IGF and (or) GH-IGF pathway or adiponectin action.

Keywords

Conjugated Linoleic Acid (CLA);Yellow-feather Broiler Chicken;Adipose Tissue;Somatropic Axis;Adiponectin

Acknowledgement

Supported by : National Natural Science Foundation of China

References

  1. Warren, J. M., V. A. Simon, G. Bartolini, K. L. Erickson, B. E. Mackey and D. S. Kelley. 2003. Trans-10, cis-12 CLA increases liver and decreases adipose tissue lipids in mice: possible roles of specific lipid metabolism genes. Lipids 38:497-504. https://doi.org/10.1007/s11745-003-1090-0
  2. Wiegand, B. R., F. C. Jr. Parrish, J. E. Swan, S. T. Larsen and T. J. Baas. 2001. Conjugated linoleic acid improves feed efficiency, decreases subcutaneous fat, and improves certain aspects of meat quality in Stress-Genotype pigs. J. Anim. Sci. 79:2187-2195. https://doi.org/10.2527/2001.7982187x
  3. Tsuboyama-Kasaoka, N., M. Takahashi, K. Tanemura, H. J. Kim, T. Tange, H. Okuyama, M. Kasai, S. Ikemoto and O. Ezaki. 2000. Conjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice. Diabetes 49:1534-1542. https://doi.org/10.2337/diabetes.49.9.1534
  4. Kang, K. and M. W. Pariza. 2001. Trans-10,cis-12-Conjugated linoleic acid reduces leptin secretion from 3T3-L1 adipocytes. Biochemical Biophysical Research Communications 287:377-382. https://doi.org/10.1006/bbrc.2001.5603
  5. Ide, T. 2005. Interaction of fish oil and conjugated linoleic acid in affecting hepatic activity of lipogenic enzymes and gene expression in liver and adipose tissue. Diabetes 54:412-423. https://doi.org/10.2337/diabetes.54.2.412
  6. Jang, L. S., H. Y. Yang and Y. H. Ko. 2004 Effect of cojugated linoleic acid on intestinal and hepatic antioxidant enzyme activity and lipid peroxidation in broiler chickens. Asian-Aust. J. Anim. Sci. 17:1162-1167. https://doi.org/10.5713/ajas.2004.1162
  7. Du, M. and D. U. Ahn. 2002. Effect of dietary conjugated linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. Poult. Sci. 81:428-433. https://doi.org/10.1093/ps/81.3.428
  8. Hausman, D. B., M. Digirolamo, T. J. Bartness, G. Hausman and R. J. Martin. 2001. The biology of white adipocyte proliferation. Obesity Review 2:239-254. https://doi.org/10.1046/j.1467-789X.2001.00042.x
  9. Kang, K., W. Liu, K. J. Albright, Y. Park and M. W. Pariza. 2003. Trans-10, cis-12 CLA inhibits differentiation of 3T3-L1 adipocytes and decreases PPAR gamma expression. Biochemical Biophysical Research Communications 303:795-799. https://doi.org/10.1016/S0006-291X(03)00413-3
  10. Lupu, F., J. D. Terwilliger, K. Lee, G. V. Segre and A. Efstratiadis. 2001. Role of growth hormone and insulin-like growth factor in mouse postnatal growth. Develop. Biol. 229:141-162. https://doi.org/10.1006/dbio.2000.9975
  11. Ohashi, A., Y. Matsushita, K. Kimura, K. Miyashta and M. Saito. 2004. Conjugated linoleic acid deteriorates insulin resistance in obese/diabetic mice in association with decreased production of adiponectin and leptin. J. Nutr. Sci. Vitaminol. (Tokyo) 50:416-421. https://doi.org/10.3177/jnsv.50.416
  12. Niswender, K. D. and M. W. Schwartz. 2003. Insulin and leptin revisited:adiposity signals with overlapping physiological and intracellular signaling capabilities. Frontiers in Neuroendocrinology 24:1-10. https://doi.org/10.1016/S0091-3022(02)00105-X
  13. Mao, J. N., J. Burnside, M. C. Postel-vinay, J. D. Pesek, J. R. Chambers and L. A. Cogburn. 1998. Ontogeny of growth hormone receptor gene expression in tissue of growth-selected strains of broiler chickens. J. Endocrinol. 156:67-75. https://doi.org/10.1677/joe.0.1560067
  14. Szymczyk, B., P. M. Pisulewski, W. Szczurek and P. Hanczakowski. 2001. Effects of conjugated linoleic acid on growth performance, feed conversion efficiency, and subsequent carcass quality in broiler chickens. Br. J. Nutr. 85:465-473. https://doi.org/10.1079/BJN2000293
  15. Parza, M. W., Y. Park and M. E. Cook. 2001. The biologically active isomers of conjugated linoleic acid. Prog. Lipid Res. 40:283-298. https://doi.org/10.1016/S0163-7827(01)00008-X
  16. Boney, C. M., R. M. Smith and P. A. Gruppuso. 1998. Modulation of insulin-like growth factor 1 mitogenic signling 3T3-L1 preadipocyte differentiation. Endocrinology 139:1638-1644. https://doi.org/10.1210/en.139.4.1638
  17. Ding, S. T., P. H. Wang, Y. H. Ko, B. H. Liu, H. M. Peng, M. Y. Lee, C. Y. Chen and Y. C. Li. 2004. The expression of porcine adiponectin and stearoyl coenzyme a desaturase genes in differentiating adipocytes. Asian-Aust. J. Anim. Sci. 17:588-593. https://doi.org/10.5713/ajas.2004.588
  18. Dai, L. H., Y. Z. Xiong, C. Y. Deng, S. W. Jiang, B. Zuo, R. Zheng, F. E. Li and M. G. Lei. 2006. Association of the A-G polymorphism in porcine adiponectin gene with fat deposition and carcass traits. Asian-Aust. J. Anim. Sci. 19:779-783. https://doi.org/10.5713/ajas.2006.779
  19. Desvergne, B. and W. Wahli. 1999. Peroxisome proliferator activated receptors: nuclear control of metabolism. Endocrine Review 20:649-688. https://doi.org/10.1210/er.20.5.649
  20. Havel, P. J. 2002. Control of energy homeostasis and insulin action by adipocyte hormones: leptin, acylation stimulating protein, and adiponectin. Current opinion in Lipidology 13:51-59. https://doi.org/10.1097/00041433-200202000-00008
  21. Scherer, P. E., S. Willias, M. Fogliano, G. Baldini and H. F. Lodish. 1995. A novel serum protein similar to C1q, produced exclusively in adipocytes. J. Biol. Chem. 270:26746-26749. https://doi.org/10.1074/jbc.270.45.26746
  22. Takahashi, Y., M. Kushiro, K. Shinohara and T. Ide. 2002. Dietary conjugated linoleic acid reduces body fat mass and affects gene expression of proteins regulating energy metabolism in mice. Comparative Biochem. Physiol. 133(3):395-404. https://doi.org/10.1016/S1096-4959(02)00164-1
  23. Zhao, R., E. Muehlbauer, E. Decuypere and R. Grossmann. 2004. Effect of genotype -nutrition interaction on growth and somatotropic gene expression in the chicken. General Compararive Endocrinology 136:2-11. https://doi.org/10.1016/j.ygcen.2003.11.009

Cited by

  1. Nutritional Factors Affecting Abdominal Fat Deposition in Poultry: A Review vol.27, pp.7, 2014, https://doi.org/10.5713/ajas.2013.13702
  2. Lipid Metabolism and Fatty Liver in Poultry vol.45, pp.2, 2018, https://doi.org/10.5536/KJPS.2018.45.2.109