Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP-HPLC

RP-HPLC를 이용한 감초에서 liquiritin, glycyrrhizic acid, glabridin의 분리

  • Tian, Minglei (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University) ;
  • Yan, Hongyuan (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University)
  • 전명래 (초정밀생물분리기술연구센터, 화공과, 인하대학교) ;
  • 염홍원 (초정밀생물분리기술연구센터, 화공과, 인하대학교) ;
  • 노경호 (초정밀생물분리기술연구센터, 화공과, 인하대학교)
  • Received : 2007.11.19
  • Accepted : 2008.02.21
  • Published : 2008.04.25

Abstract

Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0-8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.

Acknowledgement

Supported by : Inha University

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