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Cloning, Expression, and Regulation of Bovine Cellular Retinoic Acid-binding Protein-II (CRABP-II) during Adipogenesis

  • Jeong, Young Hee (Department of Food and Nutrition, Osaka City University) ;
  • Lee, Sang Mi (Department of Animal Science, Chonnam National University) ;
  • Kim, Hye-Min (Department of Animal Science, Chonnam National University) ;
  • Park, Hyo Young (Department of Animal Science, Chonnam National University) ;
  • Yoon, Duhak (National Livestock Research Institute, Rural Development Administration) ;
  • Moon, Seung Ju (Department of Animal Science, Chonnam National University) ;
  • Hosoda, Akemi (Department of Food and Nutrition, Osaka City University) ;
  • Kim, Dong-Ho (Department of Food and Nutrition, Osaka City University) ;
  • Saeki, Shigeru (Department of Food and Nutrition, Osaka City University) ;
  • Kang, Man-Jong (Department of Animal Science, Chonnam National University)
  • Received : 2007.09.18
  • Accepted : 2008.02.15
  • Published : 2008.11.01

Abstract

The mammalian cellular retinoic acid-binding proteins, CRABP-I and CRABP-II, bind retinoic acid which acts as an inducer of differentiation in several biological systems. To investigate a possible role for CRABP-II in bovine adipogenesis, we have cloned bovine CRABP-II cDNA and the coding region for CRABP-I. The predicted amino acid sequences of CRABP-II were highly conserved among several animal species (human, mouse, and rat at 97%, 93%, and 93%, respectively). The expression pattern of bovine CRABP-II was examined in greater details by applying RT-PCR to various bovine tissues. CRABP-II mRNA was expressed in most adipose-containing tissues. Moreover, the expression of CRABP-I and -II mRNA dramatically increased during the differentiation of adipocytes from bovine intramuscular fibroblast-like cells. The effects of retinoic acid on adipocyte differentiation of bovine intramuscular fibroblast-like cells were concentration-dependent. Retinoic acid activated the formation of lipid droplets at a level of 1 nM, whereas inhibition was observed at a level of $1{\mu}M$. CRABP-I gene was up-regulated and CRABP-II gene down-regulated by retinoic acid during adipocyte differentiation. These results suggest that CRABPs may play an important role in the regulation of intracellular retinoic acid concentrations during adipogenesis.

Keywords

Bovine Adipocyte Differentiation;Lipid-binding Protein;Retinoic Acid;Retinoic Acid Receptor;Retinoid X Receptor

Acknowledgement

Supported by : Rural Development Administration

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