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Retrieval of Porcine Ovarian Follicles by Different Methods

  • Choi, Moon Hwan (Gamete and Stem Cell Biotechnology, Seoul National University) ;
  • Gong, Seung Pyo (Gamete and Stem Cell Biotechnology, Seoul National University) ;
  • Lim, Jeong Mook (Gamete and Stem Cell Biotechnology, Seoul National University)
  • Received : 2006.12.16
  • Accepted : 2007.08.23
  • Published : 2008.03.01

Abstract

A series of studies has been conducted to establish a base infrastructure for an ovarian follicle culture system in the porcine and this study was designed to develop an effective retrieval protocol of preantral follicles. Five different methods using collagenase type I (A) or IV (B, C1, C2 and C3), which employed different treatment durations and/or conditions, were employed and sliced ovarian tissue of prepubertal gilts was provided for the retrieval. A significant increase in total number of follicles retrieved was detected when collagenase IV (methods B or C) was used. In total, more ovarian follicles were retrieved by method B undertaking agitation and method C2 without the agitation than method C1 and C3, while the number of preantral follicles collected was the largest in method B. Neither incubation in 5% $CO_2$ in air atmosphere instead of the agitation nor increased duration of enzymatic treatment up to 120 minutes improved the efficiency of follicle retrieval. There were no differences in the number of follicles retrieved from intact ovaries and from used ovaries for oocyte collection. These results demonstrate the collagenase IV treatment with agitation is effective for retrieving porcine preantral follicles from the ovaries.

Keywords

Porcine;Ovarian Follicle;Retrieval;Oocytes

Acknowledgement

Supported by : Rural Development Administration, Republic of Korea

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