Analysis of streptomycin in honey by LC-MS/MS

LC-MS/MS를 이용한 벌꿀 중 스트렙토마이신 분석

  • 심영은 (경기대학교 이과대학 화학과) ;
  • 명승운 (경기대학교 이과대학 화학과)
  • Received : 2008.08.01
  • Accepted : 2008.10.09
  • Published : 2008.10.25


Streptomycin, which is one of aminoglycoside antibiotics, has been widely used in the rearing of food-producing animals to prevent and treat diseases in cattle, pigs and poultry. Although not licensed in South Korea, streptomycin has also been used for the treatment of bacterial honeybee disease, such as European foulbrood in Third World countries. A reliable and effective method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of streptomycin in honey. A established method was optimized the clean-up and extraction procedure for the trace determination, good precision and accuracy. And the chromatographic and tandem mass spectrometric parameters were also optimized. The precision (RSD) and accuracy (bias) in the concentration range of 5.0~50.0 ug/kg were 5.5~14% and -10.0~8.0%, respectively. Limit of detection was 0.75 ug/kg and recovery of streptomycin spiked at level of 10 ug/kg in honey was 74%. The established and validated method was applied to determine streptomycin in honey which was on the market.


Grant : 벌꿀 중 항생제 분석법 개발 및 모니터링

Supported by : 식품의약품안전청


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