The Effect of Celosia cristata L. ethanol Extract on Anti-oxidant & Anti-aging Activity

맨드라미 (Celosia cristata L.) 에탄올 추출물이 항산화 및 항노화 작용에 미치는 효과

  • Pyo, Young-Hee (Dept. of Skin & Beauty, Osan college) ;
  • Yoon, Mi-Yun (Dept. of Skin & Beauty, Osan college) ;
  • Son, Ju-Hyun (Dept. of Bioengineering, Graduate School at Konkuk University) ;
  • Choe, Tae-Boo (Dept. of Bioengineering, Graduate School at Konkuk University)
  • Published : 2008.10.31

Abstract

For the experiment, to develop new materials for cosmetics, the Celosia cristata L. plant ethanol extract were used for physiological effect and cosmetics application research. The Celosia cristata L. is a Korean traditional variety grown. To investigate the effect of Ethanol extract of Celosia cristata L. on skin care, we measured anti-oxidant activity and anti-aging activity. Celosia cristata L. ethanol extract itself had anti-oxidant activity in a dose-dependent manner in 1-diphenyl-2-picryl-hydrazyl(DPPH) radical scavenging. Ethanol extract had anti-oxidant activity in a dose-dependent manner. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. Celosia cristata L. ethanol extract inhibited silica-induced intracellular superoxide anion generation and $H_2O_2$ generation and hydro-peroxide generation in RAW 264.7 cells. For anti-aging effects, the hyaluronidase inhibition effects, were relatively strong and they also showed elastase activity inhibition effects, which suggesting the Celosia cristata L. ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is referred that Celosia cristata L. ethanol extract appears to have potent anti-oxidant activity and anti-aging activity.

본 연구에서는 화장품 신소재 개발을 위하여 맨드라미(Celosia cristata L.) 식물의 에탄올 추출물을 이용하여 생리활성 효과 정도 및 화장품의 응용에 대한 연구를 하였다. 맨드라미는 자생력이 강하고 전국 어디서나 재배가 용이한 장점을 가지고 있으나 세포수준이나 in vivo에서 맨드라미의 항산화 및 항노화 효과에 대한 실험 결과는 보고된 바 없다. 맨드라미 추출물의 피부 자극성을 알아보기 위해 세포 독성을 관찰하였으며 맨드라미 에탄올 추출물 $100{\mu}g/m{\ell}$에서 89.5%의 비교적 낮은 세포 독성을 나타내어 사람 섬유아세포에서의 세포활성 효과가 우수하였다. DPPH를 이용한 항산화 작용측정에 있어서 맨드라미 에탄올 추출물은 농도 의존적으로 항산화 작용을 나타내었고, $100{\mu}g/m{\ell}$에서 25%의 항산화력을 보였으며, silica에 의한 RAW 264.7 세포내 superoxide anion 생성을 최고 농도인 $100{\mu}g/m{\ell}$에서 49.5%로 억제하여 강한 항산화력을 나타냈으며, DCF-DA를 이용한 세포내 $H_2O_2$ 생성을 측정한 실험에서에서도 맨드라미 에탄올 추출물은 농도 의존적으로 항산화 작용을 나타내어 $100{\mu}g/m{\ell}$에서 73%로 강력하게 항산화력을 보였다. 또한 hydroxyl radical 생성을 모두 농도 의존적으로 억제하여 $100{\mu}g/m{\ell}$에서 69.7%의 강력한 억제력을 나타내어 항산화 효능이 매우 우수하다고 보여진다. 항노화 효능에 있어서 hyaluronidase 억제 결과 농도 의존적으로 효과가 우수하여 최고 농도인 $100{\mu}g/m{\ell}$에서 40%의 높은 억제율을 보였으며, 그 외 elastase에서는 39.7%의 억제율을 나타냈다. 이는 맨드라미가 hyaluronidase나 elastase발현의 전사단계에 관련하거나 혹은 단백질 번역 후 변형 (post-translation modification) 단계에 관여하여 효소를 억제하는 효과를 나타내는 것으로 추정해 볼 수 있다. 결론적으로 자원의 재생산이 용이한 맨드라미의 식용 및 약용 자원으로서의 활용가능성을 시사하며, 맨드라미 추출물을 제조하여 화장품에 응용하면 우수한 보습제 및 항산화제, 노화 방지 화장품을 개발할 수 있다고 판단되어진다.

Keywords

References

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