Effect of Glucose at High Concentrations on the Integrin and Cathepsin Expression of the Cultured Human Periodontal Ligament Cells

고농도 포도당이 사람 치주인대세포의 Integrin과 Cathepsin 발현에 미치는 영향에 관한 연구

  • Kim, Bang-Soo (Department of Oral Anatomy, School of Dentistry, Kyung Hee University) ;
  • Shin, Je-Won (Department of Oral Anatomy, School of Dentistry, Kyung Hee University) ;
  • Hong, Jung-Pyo (Department of Oral Medicine School of Dentistry, Kyung Hee University) ;
  • Jue, Seong-Suk (Department of Oral Anatomy, School of Dentistry, Kyung Hee University)
  • 김방수 (경희대학교 치의학전문대학원 구강해부학교실) ;
  • 신제원 (경희대학교 치의학전문대학원 구강해부학교실) ;
  • 홍정표 (경희대학교 치의학전문대학원 구강내과학교실) ;
  • 주성숙 (경희대학교 치의학전문대학원 구강해부학교실)
  • Published : 2008.03.30

Abstract

This experiment was designed to clarify the effect of high concentrations of extracellular glucose on the periodontal ligament cells. The cells were incubated for 24 and 48 hours with ${\alpha}-MEM$ including 1,000 mg/L(control group) and 4,500 mg/L(experimental group) of glucose. Then, the expressions of ${\alpha}5$ integrin, cathepsin-B and -L were examined using RT-PCR method. The results were as follows: 1. ${\alpha}5$ integrin expression was increased after incubation in high glucose media. 2. Cathepsin-B expression was increased after the 24-H incubation with high glucose media, but was decreased after the 48-H incubation. 3. The expression of cathepsin-L was decreased by the high glucose media. Theses results suggest that extracellular glucose at high concentration may be attributed to delayed wound healing in diabetes patients through increase in ${\alpha}5$ integrin and decrease in cathepsins, which lead to accumulation of extracellular matrices and adhesion molecules.

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