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Effect of Growth Inhibition and Quinone Reductase Activity Stimulation of Makgeoly Fractions in Various Cancer Cells

막걸리 분획물에 의한 암세포 성장 억제 및 Quinone Reductase 활성 증가 효과

  • Shin, Mi-Ok (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Kang, Dae-Yeon (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Kim, Mi-Hyang (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University) ;
  • Bae, Song-Ja (Dept. of Food and Nutrition, Marine Biotechnology Center for Biofunctional Material Industries, Silla University)
  • 신미옥 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 강대연 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 김미향 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터) ;
  • 배송자 (신라대학교 식품영양학과, 마린-바이오 산업화지원센터)
  • Published : 2008.03.31

Abstract

In this study, we investigated the anticancer activity of Makgeoly (MG). MG was fractionated into four fractions by using solvent partition method, affording hexane (MGH), methanol (MGM), butanol (MGB) and aquous (MGA) soluble fractions. We determined the cytotoxicity of these four fractions in four kinds of cancer cell lines, such as HepG2, MCF-7, B16-F10 and HT29 by MTT assay. Among the various fractions, the MGM showed the strongest cytotoxic effects on all cancer cell lines. The morphological changes such as membrane shrinking and blebbing of cells were also observed by MGM treatment in HepG2 cell. In addition, we observed quinone reductase (QR) activity stimulating effects in all fraction layers of MG on HepG2 cells. QR activity increased approximately 2.6 and 2.1 times in MGM and MGH treated HepG2 cell at $100{\mu}g/mL$, respectively, compared to that in control value. Although further studies are needed, the present work could suggest that the fin of MG has a potential to be used as a chemopreventive agent against cancer.

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