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Protection and Detoxification Effects of Oriental Herb Extract Mixture on TCDD-Induced Oxidative Stress

TCDD로 유발된 Oxidative Stress에 대한 생약재 추출물의 방어 및 해독효과

  • Hwang, Jin-Kook (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Lee, Kyung-Jin (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Yang, Hee-Jin (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Park, Ki-Moon (Faculty of Life Science and Technology, Sungkyunkwan University)
  • 황진국 (성균관대학교 생명공학부) ;
  • 이경진 (성균관대학교 생명공학부) ;
  • 양희진 (성균관대학교 생명공학부) ;
  • 박기문 (성균관대학교 생명공학부)
  • Published : 2008.03.31

Abstract

This study was carried out to investigate the protective effects and detoxification of oriental herb extracts mixture (Saururus chinensis, Taraxacum platycarpum, Ulmus macrocarpa, Glycyrrhiza glabra, Rhynchosia nulubilis) on TCDD-induced oxidative stress. Thirty five male rats were divided into 5 groups: normal control group received saline; vehicle control group received DMSO and acetone; only TCDD-treated group received multiple intraperitoneal injection of TCDD ($100{\mu}g/kg$) and saline; post-treated group of OHEM (400 mg/kg/day) received oral administration for 5 weeks after TCDD treatment; and pre-treated group of OHEM (400 mg/kg/day) received oral administration for 6 weeks from 1 week before TCDD treatment. The elevated serum activities of alanine transaminase (AST), aspartate transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and atherorganic index due to TCDD were significantly decreased by the treatment of OHEM (p<0.05); the pre-treatment of OHEM was especially effective. Hydropic lesions and cytoplasmic vacuolizations in the liver of TCDD-treated rats were inhibited by the treatment of OHEM. Also, OHEM treatment reduced edemas in small intestine villus of TCDD-treated rats. These results suggest that OHEM from various oriental herb extracts might be a useful protective and detoxification agent against TCDD.

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