Protection and Detoxification Effects of Oriental Herb Extract Mixture on TCDD-Induced Oxidative Stress

TCDD로 유발된 Oxidative Stress에 대한 생약재 추출물의 방어 및 해독효과

  • Hwang, Jin-Kook (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Lee, Kyung-Jin (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Yang, Hee-Jin (Faculty of Life Science and Technology, Sungkyunkwan University) ;
  • Park, Ki-Moon (Faculty of Life Science and Technology, Sungkyunkwan University)
  • 황진국 (성균관대학교 생명공학부) ;
  • 이경진 (성균관대학교 생명공학부) ;
  • 양희진 (성균관대학교 생명공학부) ;
  • 박기문 (성균관대학교 생명공학부)
  • Published : 2008.03.31


This study was carried out to investigate the protective effects and detoxification of oriental herb extracts mixture (Saururus chinensis, Taraxacum platycarpum, Ulmus macrocarpa, Glycyrrhiza glabra, Rhynchosia nulubilis) on TCDD-induced oxidative stress. Thirty five male rats were divided into 5 groups: normal control group received saline; vehicle control group received DMSO and acetone; only TCDD-treated group received multiple intraperitoneal injection of TCDD ($100{\mu}g/kg$) and saline; post-treated group of OHEM (400 mg/kg/day) received oral administration for 5 weeks after TCDD treatment; and pre-treated group of OHEM (400 mg/kg/day) received oral administration for 6 weeks from 1 week before TCDD treatment. The elevated serum activities of alanine transaminase (AST), aspartate transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and atherorganic index due to TCDD were significantly decreased by the treatment of OHEM (p<0.05); the pre-treatment of OHEM was especially effective. Hydropic lesions and cytoplasmic vacuolizations in the liver of TCDD-treated rats were inhibited by the treatment of OHEM. Also, OHEM treatment reduced edemas in small intestine villus of TCDD-treated rats. These results suggest that OHEM from various oriental herb extracts might be a useful protective and detoxification agent against TCDD.


  1. Liem AK, Furst P, Rappe C. 2000. Exposure of population to dioxins and related compounds. Food Addit Contam 17: 241-259
  2. Safe S. 1995. Modulation of gene expression and endocrine response pathways by 2,3,7,8-tetrachlorodibenzo-p-dioxin and related compound. Pharmacol Ther 67: 247-281
  3. Poland A, Knutson JC. 1982. 2,3,7,8-tetrachlorodibenzo-p-dioxin and related halogenated aromatic hydrocarbons: Examination of the mechanism of toxicol. Annu Rev Pharmacol Toxicol 22: 517-554
  4. Stohs SJ. 1990. Oxidative stress induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Free Radic Biol Med 9: 79-90
  5. Jones PB, Galeazzi DR, Fisher JM, Whitlock JP Jr. 1985. Control of cytochrome P1-450 gene expression by dioxin. Science 227: 1499-1502
  6. Goeptar AR, Scheerens H, Vermeulen NP. 1995. Oxygen and xenobiotic reductase activities of cytochrome p450. Crit Rev Toxicol 25: 25-65
  7. Bradfield JY, Lee YH, Keeley LL. 1991. Cytochrome p450 family 4 in a cockroach: molecular cloning and regulation by regulation by hypertrehalosemic hormone. Proc Natl Acad Sci 88: 4558-4562
  8. Gelboin HV. 1980. Benzo[alpha]pyrene metabolism, activation and carcinogenesis: role and regulation of mixed-function oxidases and related enzymes. Physiol Rev 60: 1107-1066
  9. Kang KS, Lee YS. 1999. Effect of green tea on the male reproductive system of rat exposed 2,3,7,8-tetrachlorodibenzo-p-dioxin. International Symposium for Endocrine Disrupting Chemicals. p441-444
  10. Kim W, Hwang S, Lee H, Song H, Kim S. 1999. Panax ginseng protects the testis against 2,3,7,8-tetrachlorodibenzo-p-dioxin induced testicular damage in gunia pigs. BJU International 83: 842-849
  11. Ashida H, Fukuda I, Yamashita T, Kanszawa K. 2000. Flavons and flavonols at dietary levels inhibit a transformation of aryl hydrocarbon receptor induced by dioxin. FEBS Lett 476: 213-217
  12. Shon YH, Park IK, Moon IS, Chang HW, Park IK, Nam KS. 2002. Effect of chitosan oligosaccharide on 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in mice. Biol Pharm Bull 25: 1161-1164
  13. Ha BJ. 2003. Effects of Houttuynia Cordata thunb on antioxidative activity against TCDD damage. J Environmental Science 12: 599-603
  14. Hwang SY, Yang JB, Chang CS, Lee YC, Lee HC. 2002. Protective effect of cornu parvum extract on toxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rat. Korea J Oriental Physiology & Pathology 16: 674-679
  15. Park KM, Hwang JK, Shin KM, Kim HS, Song JH. 2003. Detoxicating effects of oriental herb extract mixtures on nicotine and dioxin. Korean J Food Sci Technol 35: 980-987
  16. Hayes MA, Roberts E, Roomi MW, Safe SH, Farber E, Cameron RG. 1984. Comparative influences of different PB-type and 3-MC-type polychlorinated biphenyl-induced phenotypes on cytocidal hepatotoxicity of bromobenzene and acetaminophen. Toxicol Appl Phamacol 76: 118-127
  17. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. 1982. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 126: 131-138
  18. Omura T, Sato R. 1964. The carbon monoxide-binding pigment of liver microsomes. II. Solubilization, purification and properties. J Biol Chem 239: 2379-2385
  19. Kociba RJ, Keeler PA, Park CN, Gehring PJ. 1976. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) results of a 13 week oral toxicity study in rats. Toxicol Appl Pharmacol 35: 553-574
  20. Hook GE, Haeeman RJK, Lucer GW. 1975. Induction and suppression of hepatic and extra-hepatic microsomal foreign compound metabolizing enzyme system by 2,3,7,8-TCDD. Chem Biol Interact 10: 199-214
  21. Cunningham HM, Williams DT. 1972. Effect of tetrachlorodibenzo-p-dioxin on growth rate and synthesis of lipid and proteins in rats. Bull Environ Contam 7: 45-51
  22. Swift LL, Gasiewicz TA, Dunn D, Soule PD, Neal RA. 1981. Characterization of the hyperlipidemia in guinea pigs induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Appl Pharmacol 59: 489-499
  23. Dibartolomeis MJ, Moore RW, Peterson RE, Jefcoate CR. 1986. Hypercholesterolemia and the regulation of Ladrenal steroidogenesis in 2,3,7,8-tetrachlorodibenzo-p-dioxin-treated rats. Toxicol Appl Pharmacol 85: 213-323
  24. Buu-Hoi NP, Harris MJA, Vos JG. 1972. Organism as targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in toxication. Naturwiss 59: 174-183
  25. Kimbrough RD, Carter CD, Liddle JA, Cline RE, Phillips PE. 1977. Epidermiology and pathology of 2,3,7,8-tetrachlorodibenzo-p-dioxin poisoning episode. Arch Environ Health 32: 77-85
  26. Kim EN, Yoo MR, Lim HJ, Hwang EW, Ha HJ, Lee HP. 2002. Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on cultured aglomerulus mesangial cell and renal tubule epithelial cell activation. Korean Journal of Nephrology 21:12-19

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