Xylanase-producing Bacillus subtilis isolated from spent mushroom (Pleurotus eryngii) substrates

큰느타리버섯 폐배지에서 분리한 Xylanase 생성 Bacillus subtilis CS9

  • Cho, Ji Jong (Division of Applied Science, Gyeongsang National University) ;
  • Hong, Su Young (Division of Hazardous Substances, National Academy of Agricultural Science) ;
  • Ha, Jun (Department of Microbiolocal Engineering. Jinju National University) ;
  • Cho, Young Un (Department of Microbiolocal Engineering. Jinju National University) ;
  • Kim, Hong Chul (Department of Microbiolocal Engineering. Jinju National University) ;
  • Gal, Sang Wan (Department of Microbiolocal Engineering. Jinju National University) ;
  • Yun, Han Dae (Division of Applied Science, Gyeongsang National University) ;
  • Cho, Soo Jeong (Department of Microbiolocal Engineering. Jinju National University)
  • 조지중 (경상대학교 응용생명과학부) ;
  • 홍수영 (농업과학기술원 농산물안전성부 유해물질과) ;
  • 하준 (진주산업대학교 미생물공학과) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 김홍출 (진주산업대학교 미생물공학과) ;
  • 갈상완 (진주산업대학교 미생물공학과) ;
  • 윤한대 (경상대학교 응용생명과학부) ;
  • 조수정 (진주산업대학교 미생물공학과)
  • Published : 2008.12.31

Abstract

A Gram-positive, endospore-forming, rod-shaped bacterial strain was isolated from spent mushroom (Pleurotus eryngii) substrates taken from the Hoamfarm located in Keyollgnam, Korea. The isolate, designated CS9, was facultatively anaerobic, motile rod and produced xylanase. The strain grew optimally at $40^{\circ}C$ and pH 6.0. The major cellular fatty acids were anteiso-$C_{15:0}$, anteiso-$C_{17:0}$, and iso-$C_{17:0}$. The genomic DNA G+C content was 45 mol%. Comparative 16S-rDNA sequence analysis showed that the isolate CS9 formed a distinct phyletic line within the genus Bacillus and was most closely related to Bacillus subtilis YB1, with 16S DNA sequence similarity of 96.8%. Sequence similarities to other type strains were 92-94%. On the based of physiological and molecular properties, the isolate CS9 was classified within the genus Bacillus as Bacillus subtilis CS9.

Keywords

Spent mushroom substrates;Pleurotus eryngii;Xylanase;Bacillus subtilis CS9